全文获取类型
收费全文 | 521篇 |
免费 | 19篇 |
专业分类
林业 | 42篇 |
农学 | 11篇 |
基础科学 | 3篇 |
110篇 | |
综合类 | 36篇 |
农作物 | 22篇 |
水产渔业 | 15篇 |
畜牧兽医 | 267篇 |
园艺 | 11篇 |
植物保护 | 23篇 |
出版年
2023年 | 6篇 |
2022年 | 6篇 |
2021年 | 15篇 |
2020年 | 12篇 |
2019年 | 17篇 |
2018年 | 12篇 |
2017年 | 22篇 |
2016年 | 16篇 |
2015年 | 14篇 |
2014年 | 16篇 |
2013年 | 15篇 |
2012年 | 33篇 |
2011年 | 45篇 |
2010年 | 27篇 |
2009年 | 24篇 |
2008年 | 30篇 |
2007年 | 28篇 |
2006年 | 26篇 |
2005年 | 20篇 |
2004年 | 16篇 |
2003年 | 14篇 |
2002年 | 13篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1981年 | 3篇 |
1976年 | 2篇 |
1973年 | 3篇 |
1972年 | 5篇 |
1971年 | 5篇 |
1969年 | 2篇 |
1967年 | 2篇 |
1932年 | 1篇 |
1927年 | 1篇 |
1906年 | 1篇 |
排序方式: 共有540条查询结果,搜索用时 15 毫秒
1.
K A Hahn D W Knapp R C Richardson C L Matlock 《Journal of the American Veterinary Medical Association》1992,200(3):355-357
In 11 dogs with nasal adenocarcinoma, IV treatment with 2 to 8 cycles of cisplatin at a dosage of 60 mg/m2 of body surface, given at 3-week intervals, resulted in a survival time of 8 to 128 weeks (mean, 32.7 weeks; median, 20 weeks). Radiography revealed complete remission in 2 dogs and partial remission in 1 dog, for an overall response rate of 27%. Clinical problems such as nasal discharge (n = 11), sneezing (n = 6), and epistaxis (n = 4) resolved in 1 to 2 weeks in all dogs after beginning chemotherapy. 相似文献
2.
D. W. Knapp B. Filion M. C. Filion N. C. Phillips 《Veterinary and comparative oncology》2005,3(1):46-47
Introduction: Mycobacterial cell wall‐DNA complex (MCC) is a bifunctional anticancer agent that induces cancer cell apoptosis and stimulates cytokine synthesis by immune cells. Intravesical MCC is currently being evaluated in humans with high‐grade urinary bladder cancer. Evaluation of MCC in dogs with transitional cell carcinoma (TCC) will allow mechanistic studies in a natural animal model of TCC, and a potentially beneficial therapy for dogs with this cancer. In this study, we have determined the anticancer activity of MCC against canine TCC cells in vitro .
Methods: Canine TCC cells (K9TCC cell line) were incubated with MCC (0.05–100 μg/ml, 0.5–72 hours). Cellular proliferation was measured by MTT reduction. Cell cycle was analyzed by flow cytometry with propidium iodide. Apoptosis was identified by flow cytometry using anti‐active‐caspase‐3/PE and anti‐cleaved‐PARP/FITC antibodies. Apoptosis‐inducing activity of 100 μg/ml MCC in combination with piroxicam (0.1–1.0 uM) was evaluated.
Results: MCC inhibited K9TCC cell proliferation in a concentration‐dependent manner (maximal activity – 45% at 100 μg/ml MCC) in association with the presence of activated caspase‐3 and cleaved PARP. Inhibition of proliferation and apoptosis‐inducing activities of MCC were independent of cell cycle phase. A thirty‐minute exposure of MCC was sufficient for optimal activity. Piroxicam (0.5 uM) enhanced apoptosis‐inducing activity of MCC.
Conclusions: MCC induces apoptosis in K9TCC cells. This activity is potentiated by piroxicam. Following positive results in vitro , in vivo studies have been initiated. One dog, treated to date, has had a minor reduction in tumor volume following the first course of treatment with no treatment‐related toxicity. 相似文献
Methods: Canine TCC cells (K9TCC cell line) were incubated with MCC (0.05–100 μg/ml, 0.5–72 hours). Cellular proliferation was measured by MTT reduction. Cell cycle was analyzed by flow cytometry with propidium iodide. Apoptosis was identified by flow cytometry using anti‐active‐caspase‐3/PE and anti‐cleaved‐PARP/FITC antibodies. Apoptosis‐inducing activity of 100 μg/ml MCC in combination with piroxicam (0.1–1.0 uM) was evaluated.
Results: MCC inhibited K9TCC cell proliferation in a concentration‐dependent manner (maximal activity – 45% at 100 μg/ml MCC) in association with the presence of activated caspase‐3 and cleaved PARP. Inhibition of proliferation and apoptosis‐inducing activities of MCC were independent of cell cycle phase. A thirty‐minute exposure of MCC was sufficient for optimal activity. Piroxicam (0.5 uM) enhanced apoptosis‐inducing activity of MCC.
Conclusions: MCC induces apoptosis in K9TCC cells. This activity is potentiated by piroxicam. Following positive results in vitro , in vivo studies have been initiated. One dog, treated to date, has had a minor reduction in tumor volume following the first course of treatment with no treatment‐related toxicity. 相似文献
3.
4.
5.
Bacterial blood culture results of 292 privately owned cats presented to the Clinic for Small Animal Medicine, Ludwig Maximilian University Munich with signs of sepsis were evaluated retrospectively. Of the blood cultures, 23% were positive. In 88%, a single bacterial species was isolated. Of all bacterial isolates, 45% were Gram-positive, 43% were Gram-negative, and 12% were obligate anaerobes. The most frequently isolated bacteria were Enterobacteriaceae, obligate anaerobic species, Staphylococcus species and Streptococcus species. Of the cats with positive blood cultures, 32% were pretreated with antibiotics. Of all bacterial isolates, 77% were susceptible to enrofloxacin, 69% to chloramphenicol, 67% to gentamicin, and 64% to amoxycillin clavulanic acid. Only enrofloxacin reached an in vitro efficacy of more than 70% against Gram-positive and more than 74% against Gram-negative bacteria. 相似文献
6.
A tool for community-based assessment of the implications of development on water security in hillside watersheds 总被引:1,自引:0,他引:1
Development and population growth in Latin American countries with steep slope farming are likely to further increase pressures on water and land resources. A methodology was developed for assessing water availability and use under different development pathways at a watershed scale to determine whether water security is a potential problem, and if so, under what conditions it is likely to occur. This methodology makes use of a GIS-based spatial water budget model for simulating stream water availability, water use and stream flow control on a daily basis at a watershed scale. Here, we analysed water availability under three plausible development scenarios for the 3246 ha Cabuyal River watershed in southwest Colombia in the year 2025: Corporate Farming (CF), Ecological Watershed (EW), and Business as Usual (BU). Simulated average river flows at the watershed outlet were, respectively, 874, 796 and 925 l s−1 for the CF, EW and BU scenarios. The contribution of base flow to river flow (base flow index) was on average, 80.8, 85.6 and 77.9%, respectively, for the three scenarios. The watershed had the potential to meet the anticipated increase in water use under each explorative scenario. However, dams were necessary to store irrigation water in the CF scenario, otherwise over 60% of the available water would have been used during the dry season. Such a high figure raises concerns about effects on aquatic and riparian ecology, concentrations of potential contaminants, water reserves for especially low rainfall years, and the watershed resilience to meet temporarily higher water needs during the day. Analyses indicated that current water-use conflicts in the watershed can be resolved if irrigation water supply is separated from drinking water supply. This study helped reduce some of the complexity associated with the interdependencies between land and water resources, the impact of using them, and spatial linkages within the watershed. Results of this study can be used for teaching local stakeholders about basic landscape responses and helping multi-institutional alliances to become proactive and to guide development to the benefit of local communities. 相似文献
7.
The C isotopic composition (δ13C) of pedogenic carbonates reflects the photosynthetic pathway of the predominant local vegetation because pedogenic (secondary) CaCO3 is formed in isotopic equilibrium with soil CO2 released by root and rhizomicrobial respiration. Numerous studies show the importance of pedogenic carbonates as a tool for reconstructing paleoecological conditions in arid and semiarid regions. The methodological resolution of these studies strongly depends on the time scale of pedogenic carbonate formation, which remains unknown. The initial formation rate can be assessed by 14C labeling of plants grown on loess and subsequent incorporation of 14C from rhizosphere CO2 into newly formed carbonate by recrystallization of loess CaCO3. We tested the feasibility of 14C and 13C tracers for estimating CaCO3 recrystallization rates by simultaneous 14C and 13C labeling and comparison with literature data. 14C labeling was more efficient and precise in assessing recrystallization rates than 13C labeling. This is connected with higher sensitivity of 14C liquid scintillation counting when compared with δ13C measurement by IRMS. Further, assessment of very low amounts of incorporated tracer is more precise with low background signal (natural abundance), which is true for 14C, but is rather high for 13C. Together, we obtained better reproducibility, higher methodological precision, and better plausibility of recrystallization rates calculated based on 14C labeling. Periods for complete CaCO3 recrystallization, extrapolated from rates based on 14C labeling, ranged from 130 (125–140) to 240 (225–255) y, while it was ≈ 600 (365–1600) y based on the 13C approach. In terms of magnitude, data from late‐Holocene soil profiles of known age provide better fit with modeled recrystallization periods based on the 14C approach. 相似文献
8.
9.
Martina Caramante Rosa Rao Luigi Maria Monti Giandomenico Corrado 《Scientia Horticulturae》2009,120(4):560-564
‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain. 相似文献
10.
Studies of earthworm species and their activity, expressed as channels, on cultivated loamy (Humic Cryaquept) and clayey soils (Typic Endoaqualf and Typic Cryaquept) were conducted in southern and central Norway before conversion from conventional to organic cropping systems. At all the three study areas: Landvik (Grimstad), Voll (Ås) and Kvithamar (Stjørdal), the earthworm species Aporrectodea caliginosa, Aporrectodea rosea, Allolobophora chlorotica and Lumbricus rubellus were found. At Landvik, where the deep-burrowing species Aporrectodea longa, A. caliginosa and Lumbricus terrestris dominated, medium (4–6 mm) and coarse (>6 mm) earthworm channels were most numerous below the plough layer. Almost no coarse pores were found at Voll and Kvithamar. The volume of earthworm channels below the plough layer was 0.6–0.8% of total soil volume at Landvik and Voll and 0.3–0.4% at Kvithamar. Earthworm channels >6 mm below the plough layer were useful to identify present and previous activity of deep-burrowing earthworms such as L. terrestris. Below the plough layer, roots were almost entirely restricted to earthworm channels and interaggregate spaces. 相似文献