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排序方式: 共有65条查询结果,搜索用时 15 毫秒
1.
Marija Marković Vladimir IvovićTijana Štajner Vitomir DjokićIvana Klun Branko BobićAleksandra Nikolić Olgica Djurković-Djaković 《Comparative immunology, microbiology and infectious diseases》2014
To contribute to the insight into the worldwide population structure of Toxoplasma gondii, we genetically characterized a total of eight strains isolated from intermediate hosts including humans, sheep and pigeons in Serbia. Although parasite DNA was detected in 28.2% (60/213) of the human samples from 162 patients serologically suspected of active toxoplasmosis, as well as in 5/7 seropositive pigeons and in 2/12 seropositive sheep examined, multilocus PCR-RFLP genotyping, using SAG1, 5′SAG2, 3′SAG2, GRA6, 5′GRA7 and 3′GRA7 as markers, was successful in only four human isolates (of which one was isolated from both the bronchoalveolar lavage fluid and blood samples of a single patient), one sheep and three pigeons. Of the eight isolates, five were type II (62.5%), one was type III, one was atypical, and one had a type I allele at GRA6 as the single locus genotyped. Although type II, as elsewhere in Europe, predominated, these results may suggest a higher genetic diversity of T. gondii in Serbia, reflecting local environmental contamination and also the geographical position of the country in South-East Europe. 相似文献
2.
Branimir Urlić Gvozden Dumičić Smiljana Goreta Ban Marija Romić 《Journal of plant nutrition》2016,39(3):389-398
Understanding the mechanisms of phosphorus (P)-use efficiency (PUE) may contribute to enhancing crop P nutrition because species growth variability at low-P is well known. The experiment was carried out to evaluate the response of kale genotypes to different P supply in randomized block design in three replications. Low-P supply led to a decrease in most parameters, whereas an increase was recorded in root growth parameters. Genotypes differed in shoot dry weight (DW), leaf area, root length and area, and shoot and root P content and concentration. Root traits significantly positively correlated with PUE. Genotypes Red Russian (RR) and IJK 17 were superior in terms of shoot DW production at low P supply, and had the highest uptake efficiency. Genotypes IJK 17 and 81 had the highest P utilization efficiency, while Vates blue curled (VBC) showed the lowest PUE. Genotypes had similar shoot P content and concentration at low P supply, but large PUE differences, implying the importance of P utilization efficiency. 相似文献
3.
Robert Brus Dalibor Ballian Peter Zhelev Marija Pandža Martin Bobinac Jane Acevski Yannis Raftoyannis Kristjan Jarni 《European Journal of Forest Research》2011,130(4):657-670
We examined leaf and mature seed cone variation of Juniperus oxycedrus L. subsp. oxycedrus in 12 natural populations across the species range in the Balkan Peninsula. We measured 10 morphological traits from a minimum
of 100 leaves in each of 190 individuals, and two morphological traits from 30–50 seed cones in each of 94 females. High phenotypic
variation was found, but no geographical structure or cline across populations was detected for any of the studied traits.
Mean values of comparable leaf and cone morphological traits did not differ considerably from values reported elsewhere. Gender
dimorphism in leaf morphology was detected, but it was not distributed uniformly throughout the studied area. An ANOVA model
with both nested and crossed effects revealed that the largest proportion of the total variation was, as expected, contained
within populations, partly as among-tree variation (18–47%, depending on the trait) and partly as within-tree variation (33–77%),
which was remarkably high. Gender dimorphism explained only 0–3% of the total variation. Differences among populations (2–23%)
were significant for all studied traits except one; however, PCA showed no clear geographical differentiation of the studied
populations. This lack of phylogeographical structure may be the consequence of repeatedly occurring colonisation-retreat
scenarios and suggests the existence of several small refugial populations scattered over a large part of the Balkan Peninsula
in the Pleistocene. Further research including palaeobotanical and molecular genetic studies will be needed to better understand
the forces that shaped current variation patterns of J. oxycedrus L. subsp. oxycedrus in the Balkan Peninsula. 相似文献
4.
The changes in the amounts of dry matter, total nitrogen and methionine during maturation of normal maize hybrids grown under the same topographical and agrotechnical conditions on two consecutive vegetation years were examined.
Total nitrogen was determined by Kjeldahl's method and methionine spectrophotometrically from papain hydrolysate by the method of McCarthy and Sullivan. Analysis of variance was applied to assess the significance of the changes in particular components during grain maturation.
A significant increase (p = 0.01) in amounts of the all investigated components in original grain matter up until a late stage of dough maturity in both vegetation years was determined. The increase was mostly expressed by the end of milky maturity. The correlation between these components during maturation is positive and highly significant (p = 0.001). The methionine content in dry matter attained in stage of milky maturation the value being characteristic for mature grain. On a total-proteins basis, the methionine content is significantly the least (p = 0.01) at the early stage of milky maturation and increases up until dough maturity reaching a value that does not significantly differ from the value in full maturity of grain. 相似文献
Total nitrogen was determined by Kjeldahl's method and methionine spectrophotometrically from papain hydrolysate by the method of McCarthy and Sullivan. Analysis of variance was applied to assess the significance of the changes in particular components during grain maturation.
A significant increase (p = 0.01) in amounts of the all investigated components in original grain matter up until a late stage of dough maturity in both vegetation years was determined. The increase was mostly expressed by the end of milky maturity. The correlation between these components during maturation is positive and highly significant (p = 0.001). The methionine content in dry matter attained in stage of milky maturation the value being characteristic for mature grain. On a total-proteins basis, the methionine content is significantly the least (p = 0.01) at the early stage of milky maturation and increases up until dough maturity reaching a value that does not significantly differ from the value in full maturity of grain. 相似文献
5.
The assessment of soil contamination and location of pollution sources represent a crucial issue in soil remediation. Topsoil samples were collected in the Zagreb area (Northwest Croatia) and the total contents of trace and major elements were determined. A multivariate geostatistical analysis was used to estimate soil chemical composition variability. Factorial Kriging Analysis (FKA) was used to investigate the scale-dependent correlation structure of some variables by modelling co-regionalization of ten chemical variables, co-kriging specific factors and mapping them. The FKA provided two regionalized factors at different spatial scales of variability: the first factor at shorter range for Zn, Pb, Cd, Cu and Ni indicated different sources of anthropogenic contamination, whereas Ca (mainly loading on the longer range factor) was related to the lithology and parent material composition. The methodology used has proved to be a useful tool to separate geological and anthropogenic causes of variation in soil heavy metal content and to identify common pollution sources. 相似文献
6.
Tessa Mazor C. Roland Pitcher Wayne Rochester Michel J. Kaiser Jan G. Hiddink Simon Jennings Ricardo Amoroso Robert A. McConnaughey Adriaan D. Rijnsdorp Ana M. Parma Petri Suuronen Jeremy Collie Marija Sciberras Lara Atkinson Deon Durholtz Jim R Ellis Stefan G. Bolam Michaela Schratzberger Elena Couce Jacqueline Eggleton Clement Garcia Paulus Kainge Sarah Paulus Johannes N. Kathena Mayya Gogina P. Daniël van Denderen Aimee A. Keller Beth H. Horness Ray Hilborn 《Fish and Fisheries》2021,22(1):72-86
Bottom trawl fishing is a controversial activity. It yields about a quarter of the world's wild seafood, but also has impacts on the marine environment. Recent advances have quantified and improved understanding of large‐scale impacts of trawling on the seabed. However, such information needs to be coupled with distributions of benthic invertebrates (benthos) to assess whether these populations are being sustained under current trawling regimes. This study collated data from 13 diverse regions of the globe spanning four continents. Within each region, we combined trawl intensity distributions and predicted abundance distributions of benthos groups with impact and recovery parameters for taxonomic classes in a risk assessment model to estimate benthos status. The exposure of 220 predicted benthos‐group distributions to trawling intensity (as swept area ratio) ranged between 0% and 210% (mean = 37%) of abundance. However, benthos status, an indicator of the depleted abundance under chronic trawling pressure as a proportion of untrawled state, ranged between 0.86 and 1 (mean = 0.99), with 78% of benthos groups > 0.95. Mean benthos status was lowest in regions of Europe and Africa, and for taxonomic classes Bivalvia and Gastropoda. Our results demonstrate that while spatial overlap studies can help infer general patterns of potential risk, actual risks cannot be evaluated without using an assessment model that incorporates trawl impact and recovery metrics. These quantitative outputs are essential for sustainability assessments, and together with reference points and thresholds, can help managers ensure use of the marine environment is sustainable under the ecosystem approach to management. 相似文献
7.
8.
Specific activity of tritium (3H) in precipitation and specific activity of 137Cs in ground-level air were monitored at three locations in Belgrade (Meteorological Station of Belgrade at Zeleno Brdo (ZB), Meteorological Station Usek (USEK), and Vin?a Institute of Nuclear Sciences (VINS)). Data presented cover the period 1985?C1997 for 137Cs for all locations and 1985?C2009 (ZB), 1988?C1997 (USEK), and 1988?C2009 (VINS) for 3H. Concentrations of 3H in precipitation have been determined using electrolytic enrichment and liquid scintillation spectrometer LKB-Wallac 1219 RackBeta. The activity of 137Cs in air was determined on an HPGe detector (Canberra, relative efficiency 23?%). 3H concentrations in precipitation ranged from 0.40?±?0.08 to 74.6?±?5.2?Bq?l?1 decreasing with distance from the nuclear facilities. Significantly higher tritium levels were measured in samples in VINS compared with those from an off-site location. The observed seasonal variations of tritium concentration indicate the stratospheric source of tritium. Increases in activity concentration of 137Cs in the atmosphere were observed after the nuclear plant accident at Chernobyl in April 1986. The concentrations obtained for 137Cs in 1986 were compared with the integrated air concentrations of 137Cs in the region. The increases of 137Cs air concentrations in 1987 and 1988 were attributed to local resuspensions from the ground. Since 1989, the activity level before the accident has been obtained. The average monthly concentrations of 137Cs in ground-level air were shown spread maximum in spring?Csummer period and pronounced maximum during winter. The obtained results were statistically analyzed, i.e., the following parameters were determined: tritium deposition, monthly activities of 3H and 137Cs, seasonal indices, radionuclide loading indices, and linear correlation coefficients. 相似文献
9.
Klun I Vujanić M Yera H Nikolić A Ivović V Bobić B Bradonjić S Dupouy-Camet J Djurković-Djaković O 《Veterinary research》2011,42(1):17
ABSTRACT: A seroepizootiological study of Toxoplasma gondii infection involving a total of 488 slaughter pigs (468 market-weight pigs and 20 sows) in the Belgrade area, also included examination of the presence of T. gondii in the blood. Blood sampled at the slaughter line was examined for specific antibodies by modified direct agglutination, and blood clots of those seropositive at titres of 1:50-1:12800 were bioassayed in mice. The overall seroprevalence was 9.2%, significantly higher (p = 0.0063) in sows (30.0%) than in market-weight pigs (8.3%). Amongst the 22 bioassays performed, a total of 16 (72.7%) were positive, by observation of T. gondii cysts (12), seropositivity (7, including 3 in which cysts were not detected), and/or detection of T. gondii DNA by real-time PCR (12, including one otherwise negative). The positive bioassays originated from the blood of 12 market-weight pigs and 4 sows. Despite a general increase in the rate of demonstration of T. gondii with the increase in the specific antibody level, the association was not significant (p = 0.101). The risk of infection was 41-fold increased in sows vs market-weight pigs, and 15-fold in pigs from smallholders' finishing type farms vs those from large farrow-to-finish farms. The presence of viable T. gondii in a proportion of the samples indicates that some of the pigs had an active parasitaemia at the time of slaughter, which, along with the seroprevalence established, points to a potential source of human infection in Serbia. This is the first report on parasitaemia in naturally infected swine. 相似文献