全文获取类型
收费全文 | 4481篇 |
免费 | 230篇 |
国内免费 | 2篇 |
专业分类
林业 | 321篇 |
农学 | 168篇 |
基础科学 | 15篇 |
1146篇 | |
综合类 | 234篇 |
农作物 | 347篇 |
水产渔业 | 471篇 |
畜牧兽医 | 1568篇 |
园艺 | 99篇 |
植物保护 | 344篇 |
出版年
2024年 | 3篇 |
2023年 | 47篇 |
2022年 | 86篇 |
2021年 | 191篇 |
2020年 | 169篇 |
2019年 | 230篇 |
2018年 | 180篇 |
2017年 | 178篇 |
2016年 | 171篇 |
2015年 | 138篇 |
2014年 | 200篇 |
2013年 | 295篇 |
2012年 | 356篇 |
2011年 | 362篇 |
2010年 | 205篇 |
2009年 | 180篇 |
2008年 | 301篇 |
2007年 | 312篇 |
2006年 | 206篇 |
2005年 | 176篇 |
2004年 | 150篇 |
2003年 | 150篇 |
2002年 | 152篇 |
2001年 | 26篇 |
2000年 | 28篇 |
1999年 | 23篇 |
1998年 | 17篇 |
1997年 | 15篇 |
1996年 | 13篇 |
1995年 | 12篇 |
1994年 | 12篇 |
1993年 | 11篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 12篇 |
1989年 | 14篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 6篇 |
1984年 | 12篇 |
1983年 | 5篇 |
1982年 | 3篇 |
1980年 | 3篇 |
1977年 | 5篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1969年 | 3篇 |
1966年 | 4篇 |
1955年 | 2篇 |
排序方式: 共有4713条查询结果,搜索用时 15 毫秒
1.
2.
A Fernandez J Perez L Carrasco M J Bautista J M Sanchez-Vizcaino M A Sierra 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1992,39(6):393-402
Lymph nodes, spleen, liver, lung and kidney obtained from pigs experimentally infected with two African Swine Fever Virus (ASFV) isolates of differing virulence were fixed by perfusion with glutaraldehyde and embedded in paraffin. An immunoperoxidase technique using a polyclonal anti-ASFV serum was performed on tissue sections in order to detect ASFV antigen. The distribution of ASFV antigen in such infected organs is shown and the differences between both infections compared and discussed. Monocytes, macrophages, hepatocytes, endothelial cells, neutrophils and epithelial cells were found to contain ASFV antigens. 相似文献
3.
4.
Nadezhda E. Ustyuzhanina Maria I. Bilan Andrey S. Dmitrenok Eugenia A. Tsvetkova Sofya P. Nikogosova Cao Thi Thuy Hang Pham Duc Thinh Dinh Thanh Trung Tran Thi Thanh Van Alexander S. Shashkov Anatolii I. Usov Nikolay E. Nifantiev 《Marine drugs》2022,20(6)
Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural characterization of polysaccharides was performed in terms of monosaccharide and sulfate content, as well as using a series of non-destructive NMR spectroscopic methods. Both FCSs were shown to contain a chondroitin core [→3)-β-d-GalNAc-(1→4)-β-d-GlcA-(1→]n bearing sulfated fucosyl branches at O-3 of every GlcA residue in the chain. These fucosyl residues were different in pattern of sulfation: FCS-BA contained Fuc2S4S, Fuc3S4S and Fuc4S at a ratio of 1:8:2, while FCS-HS contained these residues at a ratio of 2:2:1. Polysaccharides differed also in content of GalNAc4S6S and GalNAc4S units, the ratios being 14:1 for FCS-BA and 4:1 for FCS-HS. Both FCSs demonstrated significant anticoagulant activity in clotting time assay and potentiated inhibition of thrombin, but not of factor Xa. FS-BA-AT was shown to be a regular linear polymer of 4-linked α-L-fucopyranose 3-sulfate, the structure being confirmed by NMR spectra of desulfated polysaccharide. In spite of considerable sulfate content, FS-BA-AT was practically devoid of anticoagulant activity. FS-HS-AT cannot be purified completely from contamination of some FCS. Its structure was tentatively represented as a mixture of chains identical with FS-BA-AT and other chains built up of randomly sulfated alternating 4- and 3-linked α-L-fucopyranose residues. 相似文献
5.
Eckhard Koch Annegret Schmitt Dietrich Stephan Carola Kromphardt Marga Jahn Hermann-Josef Krauthausen Gustaf Forsberg Sigrid Werner Tahsein Amein Sandra A. I. Wright Federico Tinivella Maria L. Gullino Steven J. Roberts Jan van der Wolf Steven P. C. Groot 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(1):99-112
The current study was initiated to evaluate the efficacy of physical methods (hot water, aerated steam, electron treatment) and agents of natural origin (resistance inducers, plant derived products, micro-organisms) as seed treatments of carrots for control of Alternaria dauci and A. radicina. Control of both Alternaria species by seed treatment with the resistance inducers was generally poor. Results were also not satisfactory with most of the formulated commercial micro-organism preparations. Based on the average of five field trials, one of these, BA 2552 (Pseudomonas chlororaphis), provided a low but significant increase in plant stand. Among the experimental micro-organisms, the best results were obtained with Pseudomonas sp. strain MF 416 and Clonostachys rosea strain IK726. A similar level of efficacy was provided by seed treatment with an emulsion (1%) of thyme oil in water. Good and consistent control was generally achieved with the physical methods aerated steam, hot water and electron treatment. Aerated steam treatment was, apart from the thiram-containing chemical standard, the best single treatment, and its performance may at least partially be due to extensive pre-testing, resulting in dosages optimally adapted to the respective seed lot. In some of the experiments the effect of the hot water treatment, which was tested at a fixed, not specifically adapted dosage, was significantly improved when combined with a Pseudomonas sp. MF 416 or C. rosea IK726 treatment. The results are discussed in relation to the outcome of experiments in which the same seed treatment methods and agents were tested in other seed-borne vegetable pathosystems. 相似文献
6.
7.
Charles P. Woloshuk Hugh D. Sisler Maria Chrysayi Tokousbalides Samson R. Dutky 《Pesticide biochemistry and physiology》1980,14(3):256-264
Tricyclazole (5-methyl-1,2,4-triazolo[3,4-b]benzothiazole) inhibits melanin synthesis in Pyricularia oryzae at concentrations less than 0.01 μg/ml. The primary site of inhibition in the biosynthetic pathway occurs between scytalone and vermelone. Accumulation of several metabolites derived from melanin precursors along branch pathways is associated with inhibition of melanin biosynthesis. At low tricyclazole concentrations (0.01–1 μg/ml), predominant accumulation of 2-hydroxyjuglone and 3,4-dihydro-3,4,8-trihydroxy-1-(2H)-naphthalenone (3,4,8-DTN) occurs as a result of the primary block between 1,3,8-trihydroxynaphthalene and vermelone. As the concentration of tricyclazole is increased from 1 to 10 μg/ml, flaviolin accumulation is markedly enhanced, whereas that of 3,4,8-DTN and 3,4-dihydro-4,8-dihydroxy-1-(2H)-naphthalenone is depressed, indicating possible secondary sites of inhibition in the main and branch pathways. Five melanin-deficient mutants of P. oryzae that phenotypically resemble the tricyclazole-treated wild-type strain were nonpathogenic or rarely infected two rice varieties. Three of the mutants studied were genetically defective in the melanin biosynthetic pathway at the site blocked by tricyclazole in the wild type. The wild-type strain converted both scytalone and vermelone to melanin; whereas the three mutants and the tricyclazole-treated wild type converted only vermelone to melanin. The data suggest a relationship between melanin biosynthesis and pathogenicity in P. oryzae. 相似文献
8.
Marcilene Fernandes Almeida dos Santos Cleber Furlanetto Maria Rita Alves Almeida Marina Dechechi Gomes Carneiro Fabiane Castro Mota Ana Cristina Meneses Mendes Gomes Natália Orrú Reis Silveira Joelma Gardênia Pereira Silva Philippe Castagnone-Sereno Myrian Silvana Tigano Regina Maria Dechechi Gomes Carneiro 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(4):671-684
Meloidogyne incognita is one of the most polyphagous species of root-knot nematodes occurring in Brazil and worldwide. Eight M. incognita isolates were studied, representing two enzymatic phenotypes (esterase and malate desydrogenase: I1/N1, I2/N1) and four cryptic Meloidogyne sp.1 (S2/N1) isolates, representing one cytological type (3n?=?40–46). Three M. hispanica isolates (Hi3/N1, 2n?=?32–36) and two of an atypical Meloidogyne sp.2 (S2a/N3, 3n?=?40–44) were included in this study for comparison. All isolates were tested with three M. incognita-specific molecular markers. The primer pairs B06F/R, miF/R and incK14F/R amplified three species-specific fragments of 1,200?bp, 955?bp and 399?bp, respectively for M. incognita and Meloidogyne sp.1 isolates. No amplification occurred in the M. hispanica and Meloidogyne sp.2 isolates, except with primers miF/R (1,650?bp). The genetic variability of the Meloidogyne spp. isolates was evaluated, using RAPD and ISSR markers. The phylogenetic analyses revealed two strongly supported monophyletic clades: clade I, consisting of M. hispanica and the atypical Meloidogyne sp.2 isolates, and clade II, clustering together all M. incognita and the Meloidogyne sp.1 isolates. Considering the biometrical, cytological and molecular approaches, it was possible to conclude that the isolates with three enzymatic phenotypes (I1/N1, I2/N1 and S2/N1) presented the characteristics described for M. incognita. Some correlations were detected between the isozymatic phenotypes and the tree topology (S2a/N3, Hi3/N1, I1/N1, S2/N1), but no strict correlation could be observed for the phenotype I2/N1 and one isolate of S2/N1. Morphologically, the Msp.2 isolates differ from M. incognita and M. hispanica by the female stylet features presenting straight cone tip and round pear shaped knobs, posteriorly sloping. The results of this study suggested that the Msp.2 isolates with phenotypes S2aN3 belong to a new or an unidentified species closely related to M. hispanica. 相似文献
9.
Christiane von der Ohe Victoria Gauthier Lily Tamburic-Ilincic Anita Brule-Babel W. G. Dilantha Fernando Randy Clear Todd J. Ward Thomas Miedaner 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(3):407-417
Twenty four isolates of Fusarium graminearum, half of which were 3-acetyldeoxynivalenol (3-ADON) and half 15-acetyldeoxynivalenol (15-ADON) chemotypes, were tested for
their ability to produce deoxynivalenol and to cause Fusarium head blight (FHB) in spring wheat cultivars. The objectives
of this study were to determine (1) whether 3-ADON isolates differ in aggressiveness, as measured by the FHB index, and DON
production from 15-ADON isolates under field conditions, and (2) whether the performance of resistant host cultivars was stable
across isolates. Field tests of all isolates were conducted with three replicates at each of two locations in Canada and Germany
in 2008 with three host genotypes differing in FHB resistance level. The resistant host genotype showed resistance regardless
of the chemotype or location. The differences between mean FHB indices of 3-ADON and 15-ADON isolates were not significant
for any wheat genotype. In contrast, average DON production by the 3-ADON isolates (10.44 mg kg−1) was significantly (P < 0.05) higher than for the 15-ADON isolates (6.95 mg kg−1) at three of the four locations where moderately resistant lines were tested, and at both locations where susceptible lines
were evaluated. These results indicate that 3-ADON isolates could pose a greater risk to food safety. However, as the mean
aggressiveness and DON production of 3-ADON and 15-ADON chemotypes was similar on highly resistant lines, breeding and use
of highly resistant lines is still the most effective measure of reducing the risks associated with DON in wheat. 相似文献
10.