Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5

An effective host immune response to mycobacterial infection must control pathogen dissemination without inducing immunopathology. Constitutive overexpression of mycobacterial heat shock protein (myHsp70) is associated with impaired bacterial persistence, but the immune-mediated mechanisms are unknown. We found that myHsp70, in addition to enhancing antigen delivery to human dendritic cells, signaled through the CCR5 chemokine receptor, promoting dendritic cell aggregation, immune synapse formation between dendritic cells and T cells, and the generation of effector immune responses. Thus, CCR5 acts as a pattern-recognition receptor for myHsp70, which may have implications for both the pathophysiology of tuberculosis and the use of myHsps in tumor-directed immunotherapy.     

Identification of genomic locations associated with grain micronutrients (iron and zinc) in rice (Oryza sativa L.)

Genetic Resources and Crop Evolution - In this investigation, recombinant inbred lines (RILs) population derived from PAU 201 (high yielding) and Palman 579 (high iron and zinc content) varieties...     

Antigen specific immunological responses of badgers (Meles meles) experimentally infected with Mycobacterium bovis

European badgers (Meles meles) are considered to be an important reservoir of infection for Mycobacterium bovis and are implicated in the transmission of tuberculosis to cattle in Ireland and Great Britain. Accurate tests are required for tuberculosis surveillance in badger populations and to provide a basis for the development of strategies, including vaccination, to reduce the incidence of the infection. In this study, we have developed an endobronchial M. bovis infection model in badgers in which we measured cell-mediated immune and serological responses for up to 24 weeks post-infection. Groups of badgers were subjected to necropsy at 6-week intervals and the gross lesion severity status compared with immune responses measured in blood samples taken throughout the course of the study. The panel of antigens included bovine and avian tuberculins (PPD) as well as single antigens, ESAT-6, CFP-10, MPB70, Rv3019c, Rv3873, Rv3878 and Rv3879, all known to be recognised by the immune system in other animal models of tuberculosis infection. Our results demonstrated that M. bovis infected badgers responded to specific antigens as early as 6 weeks post-infection, consistent with the presence of visible lesions. The data also revealed unique patterns of antigen recognition with high levels of PBMC proliferation in the presence of CFP-10 but low proliferation levels with ESAT-6. Using a multi-antigen print immunoassay (MAPIA), we were able to confirm that MPB83 is the dominant antigen recognised by serum antibodies in infected badgers.     

Changes in amount of organic and inorganic fractions of nitrogen in an Eutrochrept soil after long‐term cropping with different fertilizer and organic manure inputs

The influence of 30 years of cropping with different fertilizer and farmyard manure (FYM) inputs on the contents and depth distribution of organic C, total N (N_t), soil mineralizable N, and organic and inorganic N fractions was investigated in an Eutrochrept. Continuous application of 100 %NPK(+S), 150 %NPK(+S), and 100 %NPK(+S)+FYM led to a marked increase in organic C, total N, hydrolyzable N (viz., amino acid N, hydrolyzable NH₄‐N, hexose amine N, and unidentified hydrolyzable N), and nonhydrolyzable N as compared to an adjacent fallow. The contents of the various organic N fractions were largest in surface soil and thereafter decreased with the depth. However, at 30 – 45 cm depth the content of organic C was not affected by the different treatments except 100 %NPK(+S)+FYM. On the other hand, continuous cropping without fertilization resulted in a depletion of total hydrolyzable N in control over fallow by 27.2 % (0–15 cm), 19.6 % (15–30 cm), and 4.7 % (30–45 cm). The incorporation of FYM with 100 %NPK(+S) resulted in greater contents of soil mineralizable N as compared to 100 %NPK(+S) (0–15, 15–30 cm). The proportion of hydrolyzable N (57–76 % of N_t) decreased and that of nonhydrolyzable N (22–40 % of N_t) increased with depth. The proportion of amino acid N (19–26 % of N_t), hexose amine N (2.1–3.5 % of N_t) and unidentified hydrolyzable N (17–27 % of N_t) decreased with depth. All organic soil N fractions including even nonhydrolyzable N in surface and subsurface soils were highly significantly correlated with soil mineralizable N derived from incubations under waterlogged and aerobic conditions. The best correlation to mineralizable N was found for amino acid N and the least significant correlation for nonhydrolyzable N.