全文获取类型
收费全文 | 327篇 |
免费 | 19篇 |
专业分类
林业 | 13篇 |
农学 | 20篇 |
122篇 | |
综合类 | 9篇 |
农作物 | 20篇 |
水产渔业 | 27篇 |
畜牧兽医 | 120篇 |
园艺 | 2篇 |
植物保护 | 13篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 12篇 |
2020年 | 13篇 |
2019年 | 16篇 |
2018年 | 11篇 |
2017年 | 17篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 12篇 |
2013年 | 17篇 |
2012年 | 23篇 |
2011年 | 32篇 |
2010年 | 18篇 |
2009年 | 14篇 |
2008年 | 25篇 |
2007年 | 20篇 |
2006年 | 15篇 |
2005年 | 18篇 |
2004年 | 17篇 |
2003年 | 7篇 |
2002年 | 12篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有346条查询结果,搜索用时 62 毫秒
1.
Nathaly Enciso Luciana L. K. Ostronoff Guillermo Mejías Leticia G. León María Luisa Fermín Elena Merino Cristina Fragio Luis Avedillo Concepción Tejero 《Veterinary research communications》2018,42(1):29-38
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25–30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration. 相似文献
2.
3.
Panfili Gianfranco Niro Serena Bufano Annarita D’Agostino Annacristina Fratianni Alessandra Paura Bruno Falasca Luisa Cinquanta Luciano 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):540-546
Plant Foods for Human Nutrition - Three wild edible plant species belonging to the Asteraceae family, Crepis vesicaria L. (s.l.), Sonchus asper (L.) Hill s.l., and Sonchus oleraceus L., usually... 相似文献
4.
Blanch Gracia Patricia Gómez-Jiménez Maria C. del Castillo Maria Luisa Ruiz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):177-183
Plant Foods for Human Nutrition - Grapes contain high contents of phenolics, which are known to possess health promoting properties. Exogenous application of phytoregulators, mainly methyl... 相似文献
5.
Inmaculada Riba Enrique García-Luque Alejandra Maz-Courrau María Luisa González de Canales Tomás Ángel DelValls 《Water, air, and soil pollution》2010,212(1-4):329-336
This study assesses the effect of salinity in bioavailability and toxicity of Zn by means of laboratory bioassays by observing contamination in both sediment and water, accumulation of Zn in biological tissues, and histopathological damage in the gills and guts tissues of Ruditapes philippinarum clams, which were exposed to different types of sediments from the Gulf of Cádiz (SW Spain) as well as two dilutions of toxic mud coming from an accidental mining spill. With this objective, the coefficients of distribution (K D) for Zn between overlying water and sediments were calculated, the histopathological frequencies in the tissues of the gills and guts of clams were determined, and the biota-sediment bioaccumulation factors as well as the bioaccumulation factors were quantified in the different stations. Results showed that the greatest histopathological damages appeared when the salinity values decreased. Statistical results showed that salinity was inversely correlated with histopathological damage (p?<?0.01) for the lesion index for gills. The most outstanding results were observed in the two dilutions of toxic mud (0.3% and 7.9%) at a salinity value of 10. Salinity was inversely correlated with the concentration of Zn in biological tissues (p?<?0.05) and inversely correlated with the concentration of Zn in water and sediment. Zn mobilization to the overlying water is produced when salinity values decrease. 相似文献
6.
Phytate degradation by lactic acid bacteria and yeasts during the wholemeal dough fermentation: a 31P NMR study 总被引:1,自引:0,他引:1
Reale A Mannina L Tremonte P Sobolev AP Succi M Sorrentino E Coppola R 《Journal of agricultural and food chemistry》2004,52(20):6300-6305
myo-Inositol hexaphosphate (IP6) is the main source of phosphorus in cereal grains, and therefore, in bakery products. Different microorganisms such as yeasts and lactic acid bacteria have phytase enzymes able to hydrolyze IP6 during the wholemeal breadmaking. In this paper, the phytase activity of Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus curvatus, and Saccharomyces cerevisiae strains, isolated from southern Italian sourdoughs, is assayed using the (31)P NMR technique. The sourdough technology based on the use of lactic acid bacteria in the breadmaking is finally suggested. 相似文献
7.
Cruciferous oilseeds are important sources of oil, proteins, and glucosinolates (GLs), potentially available when biorefinery processes are used. The proposed extraction technology is based on the use of reverse micelles (RMs) made with cetyltrimethylammonium bromide (CTAB) dispersed in organic solvent. The physicochemical properties of this extraction system and the good water solubility of many high value compounds, such as GLs and some proteins, permit the simultaneous extraction of oil, and these products from cruciferous oilseed meals. This procedure is based on three main steps: (i) seed conditioning; (ii) solid-liquid extraction by RM solution; and (iii) back-transfer of the RM solution for recovery of the extracted compounds. The method makes it possible to simultaneously extract almost the same amount of oil as with pure organic solvents used in the current extraction plants and more than 90% of soluble proteins and GLs. It is a promising biorefinery technology alternative to traditional oil extraction processes. 相似文献
8.
Atmospheric new particle formation enhanced by organic acids 总被引:4,自引:0,他引:4
Zhang R Suh I Zhao J Zhang D Fortner EC Tie X Molina LT Molina MJ 《Science (New York, N.Y.)》2004,304(5676):1487-1490
Atmospheric aerosols often contain a substantial fraction of organic matter, but the role of organic compounds in new nanometer-sized particle formation is highly uncertain. Laboratory experiments show that nucleation of sulfuric acid is considerably enhanced in the presence of aromatic acids. Theoretical calculations identify the formation of an unusually stable aromatic acid-sulfuric acid complex, which likely leads to a reduced nucleation barrier. The results imply that the interaction between organic and sulfuric acids promotes efficient formation of organic and sulfate aerosols in the polluted atmosphere because of emissions from burning of fossil fuels, which strongly affect human health and global climate. 相似文献
9.
FDI in Space Revisited: The Role of Spillovers on Foreign Direct Investment within the European Union
下载免费PDF全文
![点击此处可从《Growth and change》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Luisa Alamá‐Sabater Benedikt Heid Eduardo Jiménez‐Fernández Laura Márquez‐Ramos 《Growth and change》2017,48(3):390-408
We estimate a spatial econometric interaction model for bilateral aggregate FDI stock data between 25 European Union member countries in 2010. We find evidence for spatial spillovers of foreign direct investment for three different types of spatial dependence. Our results document FDI spillovers between neighboring countries of FDI origin countries, neighboring countries of FDI destination countries as well as between neighboring countries of both FDI origin and destination countries. Relying on recently developed methods, we provide the first model‐consistent interpretation of marginal effects of market size (measured by GDP) as well as GDP per capita on bilateral FDI activity. Our research highlights the importance of taking into account spatial lags when estimating bilateral FDI gravity models. 相似文献
10.
OBJECTIVE: To evaluate the postoperative morbidity and long-term outcome of dogs after dorsal laminectomy for caudal cervical spondylomyelopathy (CCSM). STUDY DESIGN: Retrospective study. SAMPLE POPULATION: Twenty dogs with CCSM. METHODS: Medical records of dogs treated by dorsal laminectomy for CCSM at North Carolina State University and Colorado State University between 1989 and 1999 were reviewed. Information on signalment, onset, progression and duration of clinical signs, diagnostic testing, sites of dorsal laminectomy, postoperative complications, length of hospitalization, and the ambulatory status on discharge was recorded. A minimum follow-up of 7 months was required for inclusion in the study. Neurologic status was graded (0 to 5) preoperatively, 2 days after surgery, and at the time of the study (final score). Improvement or worsening of the neurologic status was assessed by comparison of different scores for each dog. Additional follow-up information was obtained by means of a detailed telephone questionnaire directed at both the owner and referring veterinarian. RESULTS: Mean duration of clinical signs before surgery was 4.9 months. At admission, 15 dogs were ambulatory and 5 were nonambulatory. Neurologic status worsened in 70% of dogs 2 days after surgery but improved in all but 1 dog over the long term. Mean time to optimal recovery was 3.6 months. Long-term follow-up ranged from 7 months to 9 years (mean +/- SD, 3.2 +/- 2.4 years). Four dogs had confirmed recurrence; 2 other dogs may have had recurrence. CONCLUSIONS: Dorsal cervical laminectomy is an effective treatment for CCSM in those dogs with dorsal compression or multiple sites of involvement. CLINICAL RELEVANCE: Although most dogs' neurologic status transiently worsened after surgery, long-term outcome and recurrence rates were comparable to those seen with other surgical techniques for CCSM. 相似文献