Morphological and molecular characterization of Satputia (Luffa hermaphrodita Singh & Bhandari) revealed substantial diversity for use in breeding programme

Genetic Resources and Crop Evolution - Luffa hermaphrodita Singh & Bhandari, known as Satputia, a semi-wild taxon originating from L. graveolens is an underutilized vegetable. It has a...     

Multiple Induced Spawnings of the Indian Catfish Heteropneustes fossilis (Bloch) within a Prolonged Spawning Season

Abstract Singhi, the Indian catfish Heteropneustes fossilis , attained gonadal maturity repeatedly in a single spawning cycle by photothermal treatment. After the first spawning, maintenance at 30 C and 14 h light/10 h dark for 3 wk induced recruitment of the next batch of mature oocytes for subsequent spawnings. The number of eggs released declined with each spawning. However, fertilizability of the eggs was maintained until the fifth spawning. Mature fish were induced to spawn by D-Lys⁶ salmon gonadotropin-releasing hormone agonist administration. The dose of salmon gonadotropin-releasing hormone agonist for the second and subsequent spawnings was determined to be 25-fold less compared to the dose administered for the first spawning. Fish released the maximum number of eggs upon induction when they were maintained continuously at 30 C and 14 h light/10 h dark for 6 wk. The result obtained in the present study points to a possibility of obtaining multiple spawnings from those species of farmed fish which spawn once a year.     

Impact of local slope and aspect assessed from LiDAR records on tree diameter in radiata pine (Pinus radiata D. Don) plantations

Context

Reliable information on tree stem diameter variation at local spatial scales and on the factors controlling it could potentially lead to improved biomass estimation over pine plantations.

Aims

This study addressed the relationship between local topography and tree diameter at breast height (DBH) within two even-aged radiata pine plantation sites in New South Wales, Australia.

Methods

A total of 85 plots were established, and 1,302 trees were sampled from the two sites. Airborne light detection and ranging (LiDAR) was used to derive slope and aspect and to link them to each individual tree.

Results

The results showed a significant relationship between DBH and local topography factors. At both sites, trees on slopes below 20° and on southerly aspects displayed significantly larger DBHs than trees on steeper slopes and northerly aspects. Older trees with similar heights also exhibited a significant relationship between DBH and aspect factor, where greater DBHs were found on southerly aspects.

Conclusions

The observed correlation between tree DBH and LiDAR-derived slope and aspect could contribute to the development of improved biomass estimation approaches in pine plantations. These topographical variables are easily attained with airborne LiDAR, and they could potentially improve DBH predictions in resource inventories (e.g. stand volume or biomass) and support field sampling design.     

Soil erodibility and its prediction in semi-arid regions

ABSTRACT

Pedotransfer functions (PTFs) have been used to save time and cost in predicting certain soil properties, such as soil erodibility (K-factor). The main objectives of this study were to develop appropriate PTFs to predict the K-factor, and then compare new PTFs with Universal Soil Loss Equation (USLE) and the Revised Universal Soil Loss Equation (RUSLE) K-factor models. The K-factor was measured using 40 erosion plots under natural rainfall in Simakan Watershed, an area of 350 km² in central of Iran. The Regression Tree (RT) and Multiple Linear Regression (MLR) were used to develop PTFs for predicting the K-factor. The result showed that the mean of measured K was 0.01 t h MJ^?1 mm^?1. The mean K value predicted by USLE and RUSLE was 2.08 and 2.84 times more than the measured K, respectively. Although calcium carbonate was not considered in the original USLE and RUSLE K-factors, it appeared in the advanced PTFs due to its strong positive significant impact on aggregate stability and soil infiltration rate, resulting in decreased K-factor. The results also showed that the RT with R² = 0.84 had higher performance than developed MLR, USLE and RUSLE for the K estimation.     

Detection of Escherichia coli O157:H7 in cattle feces using a polymerase chain reaction-based fluorogenic 5' nuclease (TaqMan) detection assay after secondary enrichment.

Currently, methods for recovering and identifying Escherichia coli O157:H7 from cattle feces are inconsistent and hindered by their inability to specifically and rapidly detect small numbers of organisms from this complex and highly variable matrix. A standard approach for isolating and characterizing E. coli O157:H7 from cattle feces was compared with a polymerase chain reaction (PCR)-based 5' nuclease assay specific for E. coli O157:H7 that included a secondary enrichment step. The PCR-based method proved a better indicator of the presence of the organism than the culture procedure. Retests indicated that the inclusion of a secondary enrichment step and the subsequent analysis by the 5' nuclease assay were reproducible and specific. Escherichia coli O157:H7 could be detected in fecal samples that were otherwise negative after a primary enrichment step, immunomagnetic separation, and plating onto sorbitol MacConkey agar plates containing cefixime and tellurite (CT-SMAC). In samples that were initially identified as culture positive but PCR negative, retesting of the culture isolates on CT-SMAC indicated that the sorbitol fermentation interpretations could frequently not be repeated in retests, whereas retesting using the 5' nuclease assay on the original samples demonstrated a high level of agreement with the initial PCR conclusions. These results indicate the necessity of confirmatory evaluation of isolates culturally recovered by standard cultural methods that involve the interpretation of CT-SMAC. The high level of disagreement between initial culture results and retests, and the high level of agreement between initial PCR results and retests, indicates the advantages of a gene-based detection system for identifying E. coli O157:H7 in cattle feces. Screening large numbers of fecal samples for E. coli O157:H7 would appear to be feasible by integrating the use of enrichment media in serial rounds of incubation with a PCR-based fluorogenic detection procedure in high throughput detection systems that had automated liquid-handling capabilities.     

Utilizing the γ-Irradiated Sodium Alginate as a Plant Growth Promoter for Enhancing the Growth, Physiological Activities, and Alkaloids Production in Catharanthus roseus L.

Sodium alginate is a polysaccharide that is largely obtained from the brown algae (Sargassum sp.).It has been used as a wonderful growth promoting substance in its depolymerized form for various plants.The aim of this study was to find out the effects of various concentrations of γ-irradiated sodium alginate (ISA),viz.,deionized water (control,T0),20 (T1),40 (T2),60 (T3),80 (T4),and 100 ppm (T5) on the agricultural performance of Catharanthus roseus L.(Rosea) in terms of growth attributes,photosynthesis,physiological activities,and alkaloid production.The present work revealed that ISA applied as leaf-sprays at concentrations from 20 to 100 ppm might improve growth,photosynthesis,physiological activities,and alkaloid production in C.roseus L.significantly.Of the various ISA concentrations,80 ppm proved to be the best one compared to other concentrations applied.     

Impact of tillage and crop establishment methods on crop yields,profitability and soil physical properties in rice–wheat system of Indo‐Gangetic Plains of India

Conservation agriculture (CA) based on best‐bet crop management practices may increase crop and water productivity, as well as conserve and sustain soil health and natural resources. In a 2‐year study, we assessed the effects of tillage and crop establishment (TCE) methods on productivity, profitability and soil physical properties in a rice–wheat (RW) system. The six TCE treatments were used to study the impact, which are puddled transplanted rice followed by conventionally tilled wheat (CTPR–CTW), direct‐seeded rice on the flat followed by zero‐till wheat (CTDSR–ZTW), zero‐till direct‐seeded rice with residue followed by zero‐till wheat with residue (ZTDSR+R–ZTW+R), transplanted rice after rotavator puddling followed by zero‐till wheat (RTTPR–ZTW), transplanted rice after rotavator puddling followed by rotary till wheat (RTTPR–RTW) and farmer practice rice–wheat (FP–RW). Result of the study revealed that mean rice yield was not significantly affected by different TCE methods. Wheat planted with ZTDSR+R–ZTW+R gave 30% larger grain yield than FP‐RW. Overall, among all the TCE treatments, the RW system yields and net returns were maximum under ZTDSR+R–ZTW+R. The fastest mean infiltration rate (0.10 cm hr^–1) was registered in ZTDSR+R–ZTW+R plots, whereas the slowest was in FP‐RW plots (0.05 cm hr^–1). Bulk density at 15–20 cm soil depth was least in ZTDSR+R–ZTW+R (1.70 Mg m^–3) and greatest in FP‐RW (1.73 Mg m^–3). Results from this study revealed that conventionally tilled (CT) and transplanting of rice could be successfully replaced by adoption of the profitable double ZT–RW system.     

EFFECT OF APPLIED LIME AND BORON ON THE AVAILABILITY OF NUTRIENTS IN AN ACID SOIL

Productivity of resources on acid soils occupying one fourth of the total area in India is abysmally low. Lime is applied to such soils with the primary objective of increasing the productivity of crops by enhancing the availability of native and applied plant nutrients. Greenhouse pot experiments and laboratory experiments were conducted to evaluate the effects of lime and boron (B) on the availability of nutrients in soils and their uptake by plants. The application of lime enhanced the available nitrogen (N,), phosphorus (P), calcium (Ca), magnesium (Mg), sulfur (S), and zinc (Zn) content in soils, which was reflected in their uptake by sunflower (Helianthus annus). On the contrary, availability of copper (Cu), iron (Fe), and manganese (Mn) in soil was reduced due to liming. Sunflower responded very well in terms of dry matter yield to B application to the extent of 175% and 188% under 1 and 2 mg kg^?1 applied levels of B, respectively. Dry matter yield of sunflower was reduced to the tune of 29.2 and 42.7% under 2/3 and 1 lime requirement (LR), respectively, over control. Lime application at 1/3 LR with 2 mg kg^?1 of applied B emerged as an optimum combination in acid soils.     

Chemical transformation of copper in some sludge-amended soils

In a laboratory incubation study, the periodic changes in different chemical fractions of copper (Cu) in three sludge-amended soil types (acidic sandy clay loam, neutral clay loam and alkaline clay loam) of varying soil reaction were monitored under field capacity and flooding moisture regime over 16 months. The water soluble and exchangeable fraction of Cu was very low (≤1% of total Cu) in all three soil types. At the end of incubation (16 months), the sodium acetate extractable (carbonate sorbed)- and residual-Cu fractions transformed into Fe-Mn oxide fraction, irrespective of moisture regime in all three soil types. However, the extent of transformation varied among soils.