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Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm 下载免费PDF全文
The aim of this study was to test the influence of post ‐ thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post‐thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen‐thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique. 相似文献
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Two cases of Rhodococcus equl infection in foals are described, in which osteomyelitis was a feature. Because rhodococcal infection is usually low grade and chronic, and because the signs of early metaphysitis can be subtle, any articular or periarticular swelling in a foal from a farm with a history of rhodococcosis should be strongly suspected to be associated with R equl until proven otherwise. 相似文献
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A.P. Kole 《European journal of plant pathology / European Foundation for Plant Pathology》1955,61(1):159-162
European Journal of Plant Pathology - Het onderzoek had ten dcel het gedrag van de zoösporen uit de zoösporangia vanPlasmodiophora brassicae na te gaan. Aangetaste worteltjes van jonge... 相似文献
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Dr Ir A. P. Kole P. J. J. Philipsen 《European journal of plant pathology / European Foundation for Plant Pathology》1956,62(4):167-170
Samenvatting en bespreking Een vijftiental plantensoorten werd onderzocht op vatbaarheid voor het zoösporangium-stadium vanP. brassicae. Hiervan werdenTrifolium pratense, Reseda odorata enLolium perenne in zeer geringe mate, enPapaver rhoeas iets sterker aangetast. De mate van aantasting van deze laatste was echter nog aanzienlijk minder dan van als vergelijkingsobject gebruikte bloemkoolplantjes. Gezien deze resultaten is het niet waarschijnlijk, dat niet-cruciferen enigermate kunnen bijdragen tot de instandhouding of vermeerdering van de schimmel bij afwezigheid van kruisbloemigen. Evenmin is er reden te veronderstellen, dat niet-cruciferen als vangplanten voor het uitroeien van de rustsporen van groot practisch belang kunnen zijn.Summary
Webb's (1949) discovery of zoosporangia ofP. brassicae in non-crucifers raises the question of further means of multiplication and survival in the soil (MacFarlane, 1952). Perhaps the fungus can maintain itself in successive generations of zoosporangia in root hairs of these plants. From there it might in turn infect crucifers and produce a fresh crop of resting spores. This must be kept in mind when considering the possibility of eradicating the fungus from the soil by growing non-crucifers which stimulate the resting spores to germinate. If survival in root hairs were important, plants for this purpose would need to resist the zoosporangial stage as well as stimulate spore germination.In the present study a number of non-crucifers was tested for susceptibility to the zoosporangial stage by a method already described (Kole, 1955). In addition, the rate of infection of susceptible non-crucifers was compared with that of cabbage grown under the same conditions. Of seven non-crucifers, not tested before, onlyTrifolium pratense became infected.MacFaralane's findings of non-cruciferous hosts were confirmed forPapaver rhoeas, Reseda odorata andLolium perenne (Table 1). In all the susceptible non-crucifers exceptPapaver rhoeas, infection was very sligth. There were more infections onPapaver rhoeas than on the other non-crucifers but fewer than on cabbage, which was heavily infected. On the basis of these results it is not thought that the occurrence of non-cruciferous hosts ofP. brassicae will generally be of great practical importance. 相似文献
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A. P. Kole 《European journal of plant pathology / European Foundation for Plant Pathology》1965,71(3):72-78
The resting spores ofS. endobioticum function as a prosorus in germination. The contents of the resting spore are extruded as a vesicle. Within this vesicle one single sporangium develops. Germinating resting spores resemble resting spores which have been parasitized by certain fungi, but it is obvious from several facts presented that no parasites were present. On the basis of the mode of germination described,S. endobioticum should be transferred from the subgenusMesochytrium to the subgenusMicrosynchytrium. 相似文献
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