Hydroacoustic assessment of mono‐ and polymorphic Coregonus density and biomass in subarctic lakes

Density and biomass estimates of pelagic fish are essential to understand food web interactions and ecosystem functioning. We conducted surveys of six subarctic lakes for assessing both mono‐ and polymorphic whitefish Coregonus lavaretus (L.) populations. Monomorphic whitefish lakes were inhabited by a habitat and diet generalist, large sparsely rakered (LSR) morph, whereas polymorphic whitefish lakes had a littoral benthivorous LSR morph, a pelagic planktivorous densely rakered (DR) morph and in two cases a benthivorous small sparsely rakered (SSR) morph inhabiting the profundal zone. In addition, an introduced specialist zooplanktivore, vendace Coregonus albula (L.), inhabited one of the monomorphic lakes. Hydroacoustics was found to be an appropriate method for estimating coregonid densities and biomasses in large and deep polymorphic lakes occupied by the planktivorous DR morph or vendace, but only during dark nights in autumn. The suitability of hydroacoustic assessment for benthivorous LSR and SSR morphs was low, especially in polymorphic whitefish lakes due to their preference for near‐bottom habitat or shallow areas not sampled with hydroacoustics. The pelagic density of DR morph varied from 330 to 1780 fish·ha^?1 and biomass from 1.4 to 13.3 kg·ha^?1 in polymorphic whitefish lakes, whereas corresponding estimates for LSR morph were 10–320 fish·ha^?1 and 0.5–8.4 kg·ha^?1 in monomorphic whitefish lakes. In general, polymorphism tended to increase the density and biomass of whitefish in the pelagic area compared with monomorphic systems.     

On-line Analysis of Stack Gas Composition by a Low Resolution FT-IR Gas Analyzer

Stack gases of wood and oil burning boilers were analyzed by a low resolution FT-IR gas analyzer. Concentrations of carbon dioxide (CO₂), carbon monoxide (CO), nitric oxide (NO), nitrogen dioxide (NO₂), nitrous oxide (N₂O), hydrogen cyanide (HCN), sulfur dioxide (SO₂), methane (CH₄), and water vapor (H₂O) were predicted in real time by multicomponent analysis. Detection limits, linearity, analysis accuracy and long time repeatability were experimentally determined for selected gas components. Applicability of the measurement method was demonstrated by analysis of stack gas mixtures of known concentrations. The results indicate that all the primary stack gas components can be measured by the low resolution FT-IR gas analyzer with comparable results to single component measurement methods.     

The effects of winter ice cover on the trophic ecology of whitefish (Coregonus lavaretus L.) in subarctic lakes

Lakes in Arctic and subarctic regions display extreme levels of seasonal variation in light, temperature and ice cover. Comparatively little is known regarding the effects of such seasonal variation on the diet and resource use of fish species inhabiting these systems. Variation in the diet of European whitefish Coregonus lavaretus (L.) during periods of ice cover in this region is often regarded as ‘common knowledge’; however, this aspect of the species' ecology has not been examined empirically. Here, we outline the differences in invertebrate community structure, fish activity, and resource use of monomorphic whitefish populations between summer (August–September) and winter (February–March) in three subarctic lakes in Finnish Lapland. Benthic macroinvertebrate densities did not exhibit measurable differences between summer and winter. Zooplankton diversity and abundance, and activity levels of all fish species (measured as catch per unit effort) were lower in winter. The summer diet of C. lavaretus was typical of a generalist utilising a variety of prey sources. In winter, its dietary niche was significantly reduced, and the diet was dominated by chironomid larvae in all study sites. Pelagic productivity decreases during winter, and fish species inhabiting these systems are therefore restricted to feeding on benthic prey. Sampling time has strong effect on our understanding of resource utilisation by whitefish in subarctic lakes and should be taken into account in future studies of these systems.     

Substrate selection by subyearling European river lampreys (Lampetra fluviatilis) and older larvae (Lampetra spp)

More information on the habitat requirements of lamprey is needed to improve measures aiming at rehabilitation of lamprey populations. Knowledge on habitat requirements of subyearling lampreys is especially scarce. We studied substrate selection by subyearling lamprey during the first three months after hatching using aquarium experiments. Differences in substrate selection by different‐sized larvae older than one year and the substrate depth selection by large larvae were also studied. Prolarvae of an average total length (TL) of 6.9 mm actively selected substrates with existing holes. When lampreys reached TL of over 8.0 mm, they started to select substrates which enabled them to construct a burrow. Lampreys of that size avoided coarse sand and gravel without fine material. Natural gravel with fine material was a frequently selected burrowing substrate during the 10‐week experiment suggesting its importance for subyearling lampreys. Substrates where very fine sand prevailed were the most selected soft sediments by subyearling larvae. Larvae older than one year did not select clay as a burrowing substrate. Medium (TL 70–98 mm) and large (TL 110–146 mm) larvae preferred coarse organic matter to fine sand. Larvae older than one year (TL 80–110 mm) preferred the thickest layer of soft substrate (10 cm) available to thinner layers.     

A novel method to analyze betaine in chicken liver: effect of dietary betaine and choline supplementation on the hepatic betaine concentration in broiler chicks

Betaine was measured from liver tissue by a high-performance liquid chromatographic (HPLC) method developed for this study. The method involves homogenization of liver in acetate buffer at pH 6 and precipitation of protein with trichloroacetic acid, which was removed by diethyl ether extraction. Betaine was separated using a cation exchange column in Ca(2+) form and detected with a refractive index detector. This method also allows the determination of S-adenosylmethionine (S-AM) from the same liver extract but with different HPLC conditions. Broiler chicks were fed with experimental diets supplemented with four different doses of betaine or choline ranging from 0 to 5 mol equiv. After a 3 week feeding period, the livers were analyzed for betaine and S-AM. Dietary betaine was twice as efficient in increasing the hepatic betaine concentration as dietary choline. The hepatic S-AM concentrations were similar in all dietary treatments.     

Electroencephalography findings in healthy and Finnish Spitz dogs with epilepsy: visual and background quantitative analysis

BACKGROUND: Qualitative and quantitative electroencephalography (EEG) parameters of healthy and Finnish Spitz dogs with epilepsy have not been determined. OBJECTIVE: To determine if EEG can provide specific characteristics to distinguish between healthy dogs and dogs with epilepsy. ANIMALS: Sixteen healthy and 15 Finnish Spitz dogs with epilepsy. METHODS: A prospective clinical EEG study performed under medetomidine sedation. Blinded visual and quantitative EEG analyses were performed and results were compared between study groups. RESULTS: Benign epileptiform transients of sleep and sleep spindles were a frequent finding in a majority of animals from both groups. The EEG analysis detected epileptiform activity in 3 Finnish Spitz dogs with epilepsy and in 1 healthy Finnish Spitz dog. Epileptiform activity was characterized by spikes, polyspikes, and spike slow wave complexes in posterior-occipital derivation in dogs with epilepsy and with midline spikes in control dog. The healthy dogs showed significantly less theta and beta activity than did the dogs with epilepsy (P < .01), but the only significant difference between healthy dogs and dogs with untreated epilepsy was in the alpha band (P < .001). Phenobarbital treatment increased alpha, beta (P < .001), and theta (P < .01), and decreased delta (P < .001) frequency bands compared with dogs with untreated epilepsy. CONCLUSIONS AND CLINICAL IMPORTANCE: Benign epileptiform transients of sleep could be easily misinterpreted as epileptiform activity. Epileptiform activity in Finnish Spitz dogs with epilepsy seems to originate from a posterior-occipital location. The EEG of dogs with epilepsy exhibited a significant difference in background frequency bands compared with the control dogs. Phenobarbital treatment markedly influenced all background activity bands. Quantitative EEG analysis, in addition to visual analysis, seems to be a useful tool in the examination of patients with epilepsy.     

Using multi-source data to map and model the predisposition of forests to wind disturbance

Natural disturbances such as wind are known to cause threats to ecosystem services as well as sustainable forest ecosystem management. The objective of this research was to better understand and quantify drivers of predisposition to wind disturbance, and to model and map the probability of wind-induced forest disturbances (P_DIS) in order to support forest management planning. To accomplish this, we used open-access airborne light detection and ranging (LiDAR) data as well as multi-source National Forest Inventory (NFI) data to model P_DIS in southern Finland. A strong winter storm occurred in the study area in December 2011. High spatial resolution aerial images, acquired after the disturbance event, were used as reference data. Potential drivers associated with P_DIS were examined using a multivariate logistic regression model. The model based on LiDAR provided good agreement with detected areas susceptible to wind disturbance (73%); however, when LiDAR was combined with multi-source NFI data, the results were more promising: prediction accuracy increased to 81%. The strongest predictors in the model were mean canopy height, mean elevation, and stem volume of the main tree species (Norway spruce and Scots pine). Our results indicate that open-access LiDAR data can be used to model and map the probability of predisposition to wind disturbance, providing spatially detailed, valuable information for planning and mitigation purposes.     

The effect of feeding essential oils on broiler performance and gut microbiota

1.?In this study the effect of a blend of essential oils (EO) comprising 15 g/tonne thymol and 5 g/tonne cinnamaldehyde on the performance and intestinal microbiota of broilers was investigated.

2.?A total of 720 male Ross broilers were divided into two dietary treatments with 12 replicate pens per treatment. Broilers were given a control soybean-wheat-based diet with or without added EO in two diet phases (0–21 d and 22–42 d).

3.?The blend of EO increased body weight gain of broilers from 0 to 42 d by 4?5%.

4.?Caecal microbiota were affected by the EO blend; in particular increases in the proportions of Lactobacillus and Escherichia coli at 41 d was observed.

5.?The EO blend had major effects on caecal metabolites. The proportion of caecal butyrate at 20 and 41 d of age increased, whereas the proportion of caecal acetic acid at 20 d, and propionic acid and isovaleric acid at 41 d, decreased with the EO blend. In addition, the caecal proportion of spermine increased and tyramine decreased at 41 d of age with the EO treatment.

6.?The present study shows that EO supplementation exerts a positive effect on intestinal microbiota with a concomitant enhancement in growth performance. The study suggests that modulation of broiler gut microbiota composition and activity through the administration of EO offers an effective means for improving broiler performance.     

Effect of emulsion characteristics on the release of aroma as detected by sensory evaluation,static headspace gas chromatography,and electronic nose

The effects of emulsion structure and composition of the matrix on the release of linalool (nonpolar) and diacetyl (polar) were studied using sensory evaluation, static headspace gas chromatography, and an electronic nose. The matrices used were water, rapeseed oil, and eight oil-in-water emulsions differing in oil volume fraction (0.05/0.5), emulsifier type (sucrose stearate/modified potato starch), and homogenization pressure (100/300 bar). Fat content strongly affected the release of linalool, but it was not as critical a factor in the release of the more polar compound, diacetyl. A slight effect of the emulsifier type on the release of aromas was observed with sensory and gas chromatographic methods. The reduced droplet size, resulting from higher homogenization pressure, enhanced the release of linalool but had no effect on diacetyl. Sensory and gas chromatographic methods detected aroma changes quite similarly. The electronic nose was capable of detecting only the effect of fat on linalool.     

Carbon stock changes of forest land in Finland under different levels of wood use and climate change

Context

Prediction of the effect of harvests and climate change (CC) on the changes in carbon stock of forests is necessary both for CC mitigation and adaptation purposes.

Aims

We assessed the impact of roundwood and fuelwood removals and climate change (CC) on the changes in carbon stock of Finnish forests during 2007–2042. We considered three harvest scenarios: two based on the recent projections of roundwood and fuelwood demand, and the third reflecting the maximum sustainable cutting level. We applied two climate scenarios: the climate was in the state that prevailed around year 2006, or it changed according to the IPCC SRES A1B scenario.

Methods

We combined the large-scale forestry model MELA with the soil carbon model Yasso07 for mineral soils. For soils of drained, forested peatlands, we used a method based on emission factors.

Results

The stock change of trees accounted for approximately 80 % of the total stock change. Trees and mineral soils acted as carbon sinks and the drained peatland soils as a carbon source. The forest carbon sink increased clearly in both of the demand-based scenarios, reaching the level of 13–20 Tg C/year (without CC). The planned increase in the use of bioenergy reduced the forest sink by 2.6 Tg C/year. CC increased the forest carbon sink in 2042 by 38 %–58 % depending on the scenario. CC decreased the sink of mineral soils in the initial years of the simulations; after 2030, the effect was slightly positive. CC increased the emissions from the drained peatland soils.

Conclusions

It is likely that forest land in Finland acts as a carbon sink in the future. The changes in carbon stocks of trees, mineral soils, and peatland soils respond differently to CC and fuelwood and roundwood harvests.