全文获取类型
收费全文 | 78篇 |
免费 | 3篇 |
专业分类
林业 | 1篇 |
农学 | 7篇 |
22篇 | |
综合类 | 2篇 |
农作物 | 11篇 |
水产渔业 | 8篇 |
畜牧兽医 | 14篇 |
园艺 | 2篇 |
植物保护 | 14篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 3篇 |
2014年 | 4篇 |
2013年 | 13篇 |
2012年 | 5篇 |
2011年 | 9篇 |
2010年 | 2篇 |
2008年 | 5篇 |
2007年 | 2篇 |
2006年 | 3篇 |
2005年 | 1篇 |
2004年 | 1篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有81条查询结果,搜索用时 15 毫秒
1.
T. Ahmad S.P. Singh B.K. Khangembam J.G. Sharma R. Chakrabarti 《Aquaculture Nutrition》2014,20(3):265-272
The effect of temperature on the food consumption rate and the digestive enzyme activities of Clarias batrachus (80.60 ± 5.34 g) were evaluated. Fish were exposed to six different temperatures of 10, 15, 20, 25, 30 and 35 °C following an acclimation temperature of 25 °C. The rate of temperature change was 2 °C day?1. Highest food consumption was recorded at 25 °C. It gradually reduced with decreasing water temperature. Food consumption rate was significantly (P < 0.05) lower at 10 °C compared with other treatments. Hence, 46.67, 8.20–23.58 and 1.02–6.15% reduced food consumptions were recorded in groups exposed at 10, 15 and 20 °C temperatures, respectively, compared with the 25 °C. The consumption rate was not affected in fish exposed at 30 and 35 °C. Total protease, trypsin and chymotrypsin activities were significantly (P < 0.05) higher in fish exposed at 25 °C compared with others. Lipase activity was significantly (P < 0.05) higher in fish exposed at 30 °C compared with others. Lowest enzyme activities were recorded at 10 °C. Water temperature below 25 °C affected the food consumption and digestive enzyme activities in fish that served as indicators of stress in fish. 相似文献
2.
Raigond Baswaraj Pathania Shruti Verma Ambika Verma Gaurav Kochhar Tarvinder Chakrabarti S. K. 《植物病害和植物保护杂志》2021,128(4):1061-1071
Journal of Plant Diseases and Protection - Recombinase Polymerase Amplification (RPA) assay was developed for specific detection of Tomato leaf curl New Delhi virus-potato (ToLCNDV [potato]),... 相似文献
3.
Roy D Liston DR Idone VJ Di A Nelson DJ Pujol C Bliska JB Chakrabarti S Andrews NW 《Science (New York, N.Y.)》2004,304(5676):1515-1518
Strategies for inhibiting phagolysosome fusion are essential for the intracellular survival and replication of many pathogens. We found that the lysosomal synaptotagmin Syt VII is required for a mechanism that promotes phagolysosomal fusion and limits the intracellular growth of pathogenic bacteria. Syt VII was required for a form of Ca2+-dependent phagolysosome fusion that is analogous to Ca2+-regulated exocytosis of lysosomes, which can be triggered by membrane injury. Bacterial type III secretion systems, which permeabilize membranes and cause Ca2+ influx in mammalian cells, promote lysosomal exocytosis and inhibit intracellular survival in Syt VII +/+ but not -/- cells. Thus, the lysosomal repair response can also protect cells against pathogens that trigger membrane permeabilization. 相似文献
4.
Chakrabarty S Das A Bhattacharya A Chakrabarti G 《Journal of agricultural and food chemistry》2011,59(5):2040-2048
Here we studied the antiproliferative activity of theaflavins in cervical carcinoma HeLa cells by investigating their effects on cellular microtubules and purified goat brain tubulin. Theaflavins inhibited proliferation of HeLa cells with IC(50) value of 110 ± 2.1 μg/mL (p = < 0.01), caused cell cycle arrest at G(2)/M phase and induced apoptosis with alteration of expression of pro- and antiapoptotic proteins. Along with these antiproliferative activities, theaflavins act as microtubule depolymerizers. Theaflavins disrupted the microtubule network accompanied by alteration of cellular morphology and also decreased the polymeric tubulin mass of the cells. The polymerization of cold treated depolymerized microtubules in HeLa cells was prevented in the presence of theaflavins. In vitro polymerization of purified tubulin into microtubules was also inhibited by theaflavins with an IC(50) value of 78 ± 2.43 μg/mL (P < 0.01). Thus, disruption of cellular microtubule network of HeLa cells through microtubule depolymerization may be one of the possible mechanisms of antiproliferative activity of theaflavins. 相似文献
5.
S. Tripathi A. Chakraborty B. K. Bandyopadhyay K. Chakrabarti 《Archives of Agronomy and Soil Science》2013,59(4):439-450
The studied soils were collected from a 24-year long-term fertilizer experiment with rice (Oryza sativa L.) grown on tropical Typic Endoaquepts during monsoon season only. The organic carbon (OC) and total nitrogen (TN) status of the soils improved with the N100P22K42 treatment than the initial values at the start of the experiment. The available P status of the soils depleted in the N100P11K22 and N0P0K0 treatments than the initial value. Soil microbial biomass carbon (MBC) and basal soil respiration (BSR), activities of urease, acid and alkaline phosphatases as well as fluorescein diacetate hydrolyzing activity (FDHA) were the highest in the N100P22K42 treatment. The highest percentage of MBC in OC in the N100P22K42 treatment revealed the accumulation of organic matter with an overall improvement in nutrient status of soil, since MBC is a labile pool of plant nutrients. Long-term application of fertilizers (N100P22K42) had no deleterious effect on microbial and biochemical soil quality parameters. Rice grain yields were inconsistent with N100P0K0 and N0P0K0 while that of N100P22K42, N100P11K22 and N100P22K0 increased gradually during the course of the experiment for each block period of six years. The grain yield of rice showed high significant correlation with the microbial and biochemical soil parameters. 相似文献
6.
7.
Jagesh K. Tiwari Sundaresha Siddappa Bir Pal Singh Surinder K. Kaushik Swarup K. Chakrabarti Vinay Bhardwaj Poonam Chandel 《Plant Breeding》2013,132(3):237-245
Late blight is the most devastating disease of the potato crop that can be effectively managed by growing resistant cultivars. Introgression of resistance (R) genes/quantitative trait loci (QTLs) from the Solanum germplasm into common potato is one of the plausible approaches to breed resistant cultivars. Although the conventional method of breeding will continue to play a primary role in potato improvement, molecular marker technology is becoming one of its integral components. To achieve rapid success, from the past to recent years, several R genes/QTLs that originated from wild/cultivated Solanum species were mapped on the potato genome and a few genes were cloned using molecular approaches. As a result, molecular markers closely linked to resistance genes or QTLs offer a quicker potato breeding option through marker‐assisted selection (MAS). However, limited progress has been achieved so far through MAS in potato breeding. In near future, new resistance genes/QTLs are expected to be discovered from wild Solanum gene pools and linked molecular markers would be available for MAS. This article presents an update on the development of molecular markers linked to late blight resistance genes or QTLs by utilization of Solanum species for MAS in potato. 相似文献
8.
Effect of cortisol and triiodothyronine bath treatments on the digestive enzyme profile and growth of Catla catla larvae during ontogenic development
下载免费PDF全文
![点击此处可从《Aquaculture Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Bronson Kumar Khangembam Arun Shivnath Ninawe Rina Chakrabarti 《Aquaculture Research》2017,48(5):2173-2185
Digestive enzyme profile is a good indicator of the nutritional and health status of the fish. The present investigation aims to evaluate the effect of exogenous bath treatment of hormones, cortisol and triiodothyronine, on the digestive enzyme activities and growth of carp Catla catla (Ham.) during ontogenic development. Catla larvae (4 days old) were given bath treatment with cortisol (hydrocortisone, 0.2 mg L?1), 3,5,3′‐triiodothyronine (T3, 2.5 mg L?1) and a combination of cortisol and T3 for 30 min. Digestive enzyme profile was recorded on every third day and was continued for 30 days. Larvae were fed with live food for initial 14 days and then weaned to mix feeding of live food and prepared diet. Significantly (P < 0.05) higher amylase, total protease, trypsin, chymotrypsin, lipase, chitinase and chitinobiase activities were found in the hormone‐treated groups compared to the control one during ontogenic development. Among the treated groups, amylase activity was highest in cortisol‐treated larvae. Total protease, trypsin, chymotrypsin, lipase, chitinase and chitinobiase activities were significantly (P < 0.05) higher in larvae exposed at combined treatment of cortisol and T3 compared to the other two groups in most sampling days. Average length, weight and specific growth rate of treated larvae were higher compared to the control one. The combined bath treatment of cortisol and triiodothyronine influenced the digestive enzyme activities of catla larvae and thereby enhanced the growth at early developmental stage. This helps the larvae to overcome the problems associated with early developmental stage. 相似文献
9.
Bradley N Metz Priyadarshini Chakrabarti Ramesh R Sagili 《Journal of insect science (Online)》2021,21(6)
Honey bee larvae are dependent on the social structure of colony for their provisioning and survival. With thousands of larvae being managed collectively by groups of foragers (collecting food resources) and nurse bees (processing food and provisioning larvae), coordination of colony efforts in rearing brood depends on multiple dynamic cues of larval presence and needs. Much of these cues appear to be chemical, with larvae producing multiple pheromones, major being brood ester pheromone (BEP; nonvolatile blend of fatty acid esters) that elicits both short-term releaser effects and long-term primer effects. While BEP can affect colony food collection and processing with the signaling of larval presence, it is unclear if BEP signals individual larval needs. To understand this aspect, in a series of experiments we manipulated larval feeding environment by depriving larvae from adult bee contact for 4-h period and examined (1) nurse bee interactions with contact-deprived and nondeprived larvae and larval extracts; (2) forager bee responses to contact-deprived and nondeprived larval extracts. We also characterized BEP of contact-deprived and nondeprived larvae. We found that nurse honey bees tend to aggregate more over contact-deprived larvae when compared with nondeprived larvae, but that these effects were not found in response to whole hexane extracts. Our analytical results suggest that BEP components changed in both quantity and quality over short period of contact deprivation. These changes affected foraging behavior, but did not appear to directly affect nursing behavior, suggesting that different chemical cues are involved in regulating nursing effort to individual larvae. 相似文献
10.
Arjunan Jeevalatha Priyanka Kaundal Ravinder Kumar Baswaraj Raigond Rakesh Kumar Sanjeev Sharma Swarup Kumar Chakrabarti 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(3):565-573
Apical leaf curl disease of potato is caused by a whitefly transmitted begomovirus, Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) in India. Detection of this virus is essential to manage the disease, particularly in healthy potato seed production systems. Large scale testing of micro-plants demands a simple, rapid and sensitive assay. Hence, loop-mediated isothermal amplification (LAMP) method was developed for specific detection of ToLCNDV-[potato]. Six primers that recognize the coat protein gene sequence of ToLCNDV-[potato] were designed and LAMP assay was optimized using different concentrations of magnesium sulphate, betaine, dNTPs, Bst DNA polymerase and temperature. The results were assessed by visual observation of turbidity, colour change using SYBR green dye and also by gel electrophoresis. The assay successfully detected the virus in infected plants collected from potato fields whereas no cross-reactions were observed with healthy plants and other potato viruses. The optimized assay was as sensitive as PCR assay and could detect up to 0.002 pg of total DNA. The assay could detect the virus in infected potato tubers and also in asymptomatic plants. Print-capture LAMP assay was developed and its application could reduce the cost and time of the assay in large scale testing under seed production. 相似文献