Starving the soil of plant inputs for 50 years reduces abundance but not diversity of soil bacterial communities

If soil communities rely on plant-derived carbon, is biodiversity lost when this primary source is removed? Soil microbial and mesofaunal communities at the Rothamsted Highfield site were compared under a mixed grass sward, arable rotation and a section maintained as a bare-fallow for the past 50 years by regular tillage. Organic matter reserves have been degraded and microbial and mesofaunal numbers and mite diversity have declined in this unique bare-fallow site, where fresh carbon inputs have been drastically reduced. However, it supports a species-rich metabolically active bacterial community of similar diversity to that in soil maintained as grass sward. Thus in contrast to soil mesofauna, bacterial diversity (but not abundance) is apparently independent of plant inputs.     

Yield and soil system changes from conservation tillage in dryland farming: A case study from North Eastern Tanzania

Yield levels in smallholder farming systems in semi-arid sub-Saharan Africa are generally low. Water shortage in the root zone during critical crop development stages is a fundamental constraining factor. While there is ample evidence to show that conservation tillage can promote soil health, it has recently been suggested that the main benefit in semi-arid farming systems may in fact be an in situ water harvesting effect. In this paper we present the result from an on-farm conservation tillage experiment (combining ripping with mulch and manure application) that was carried out in North Eastern Tanzania from 2005 to 2008. Special attention was given to the effects of the tested treatment on the capacity of the soil to retain moisture. The tested conservation treatment only had a clear yield increasing effect during one of the six experimental seasons (maize grain yields increased by 41%, and biomass by 65%), and this was a season that received exceptional amounts of rainfall (549 mm). While the other seasons provided mixed results, there seemed to be an increasing yield gap between the conservation tillage treatment and the control towards the end of the experiment, and cumulatively the yield increased with 17%. Regarding soil system changes, small but significant effects on chemical and microbiological properties, but not on physical properties, were observed. This raises questions about the suggested water harvesting effect and its potential to contribute to stabilized yield levels under semi-arid conditions. We conclude that, at least in a shorter time perspective, the tested type of conservation tillage seems to boost productivity during already good seasons, rather than stabilize harvests during poor rainfall seasons. Highlighting the challenges involved in upgrading these farming systems, we discuss the potential contribution of conservation tillage towards improved water availability in the crop root zone in a longer term perspective.     

Changes in the volatile composition of a semihard ewe milk cheese induced by high-pressure treatment of 300 MPa

The effect of high-pressure (HP) treatment (300 MPa, 10 min) on the volatile profile of semihard ewe milk cheeses was investigated. The HP treatment was applied at two different stages of ripening (1 and 15 days; 3P1 and 3P15) and microbiota, proteolysis indexes (soluble nitrogen and total free amino acid content), and volatile compounds were assayed at 15, 60, 90, and 150 days of ripening. The intensity of odor and aroma of cheeses was also assayed. 3P1 cheeses presented the highest content of free amino acids and were characterized by the lowest amounts of aldehydes, ketones, short-chain free fatty acids, and terpenes and higher levels of ethanol and ethyl esters. 3P15 cheeses were characterized by the highest content of short-chain free fatty acids and pyruvaldehyde and the lowest abundance of secondary alcohols and were more similar to control cheeses than those HP-treated on the first day. Intensities of odor and aroma were not significantly influenced by the HP treatment. However, the panellists found some differences in 3P1 as compared with control and 3P15 cheeses in what they perceived as lower odor and aroma quality.     

Technical note: a double L intestinal cannula for cattle

A double L-shaped intestinal cannula was developed in an attempt to overcome problems observed previously with simple T-type cannulas. The cannula was constructed from cyclopolyvinyl chloride water pipe fittings. Construction materials were fairly rigid, but by connecting the split cannula pieces with elastic castration bands the cannula had some flexibility. Placing a short cone over the exposed cannula barrel reduced mechanical damage to the intestine. The double L cannula required a much smaller incision in the intestine during surgical insertion than a T-type cannula; it also simplified replacement. Construction is described; use and performance of the cannula has been satisfactory.     

Land Cover Transition in Northern Tanzania

Land conversion in sub‐Saharan Africa has profound biophysical, ecological, political and social consequences for human well‐being and ecosystem services. Understanding the process of land cover changes and transitions is essential for good ecosystem management policy that would lead to improved agricultural production, human well‐being and ecosystems health. This study aimed to assess land cover transitions in a typical semi‐arid degraded agro‐ecosystems environment within the Pangani river basin in northern Tanzania. Three Landsat images spanning over 30 years were used to detect random and systematic patterns of land cover transition in a landscape dominated by crop and livestock farming. Results revealed that current land cover transition is driven by a systematic process of change dominated by the following: (i) transition from degraded land to sparse bushland (10·8%); (ii) conversion from sparse bushland to dense bushland in lowland areas (6·0%); (iii) conversion from bushland to forest (4·8%); and (iv) conversion from dense bushland to cropland in the highlands (4·5%). Agricultural lands under water harvesting technology adoption show a high degree of persistence (60–80%) between time slices. This suggests that there is a trend in land‐use change towards vegetation improvement in the catchment with a continuous increase in the adoption of water harvesting technologies for crop and livestock farming. This can be interpreted as a sign of agricultural intensification and vegetation regrowth in the catchment. Copyright © 2015 John Wiley & Sons, Ltd.     

Transgenic inhibition of synaptic transmission reveals role of CA3 output in hippocampal learning

The hippocampus is an area of the brain involved in learning and memory. It contains parallel excitatory pathways referred to as the trisynaptic pathway (which carries information as follows: entorhinal cortex --> dentate gyrus --> CA3 --> CA1 --> entorhinal cortex) and the monosynaptic pathway (entorhinal cortex --> CA1 --> entorhinal cortex). We developed a generally applicable tetanus toxin-based method for transgenic mice that permits inducible and reversible inhibition of synaptic transmission and applied it to the trisynaptic pathway while preserving transmission in the monosynaptic pathway. We found that synaptic output from CA3 in the trisynaptic pathway is dispensable and the short monosynaptic pathway is sufficient for incremental spatial learning. In contrast, the full trisynaptic pathway containing CA3 is required for rapid one-trial contextual learning, for pattern completion-based memory recall, and for spatial tuning of CA1 cells.     

Actin-myosin interaction: inhibition of the myosin adenosine triphosphatase by actin

In the absence of magnesium ion, the addition of actin to myosin in a 1 :4 ratio has a strong inhibitory effect on the adenosine triphosphatase activity, in contrast to the well-known activating effect of actin in the presence of magnesium ion. This finding suggests that both effects result from a conformational change in the active site of the myosin adenosine triphosphatase.