Hydrological Responses and Flow Pathways in an Acrisol on a Forested Hillslope with a Monsoonal Subtropical Climate

The nature of subsurface flow depends largely on hydraulic conductivity of the vadoze zone, permeability of the underlying bedrock, existence of soil layers differing in hydraulic properties and macropore content, soil depth, and slope angle.Quantification of flow pathways on forested hillslopes is essential to understand hydrological dynamics and solute transport patterns.Acrisols, with their argic Bt horizons, are challenging in this respect.To further elucidate flow pathways of water and short-term variability of soil moisture patterns in Acrisols, a field study was conducted on a forested hillslope in a sub-catchment of the Tie Shan Ping(TSP)watershed, 25 km northeast of Chongqing City, China.This catchment is covered by a mixed secondary forest dominated by Masson pine(Pinus massoniana).Soil saturated hydraulic conductivity(K sat) was significantly reduced at the interface between the AB and Bt horizons(2.6 × 10~(-5) vs.1.2 × 10~(-6) m s~(-1)), which led to that the flow volume generated in the Bt horizon was of little quantitative importance compared to that in the AB horizon.There was a marked decrease in porosity between the OA and AB horizons, with a further decrease deeper in the mineral subsoil.Especially, the content of soil pores 300 μm was higher in the AB horizon(14.3%)than in the Bt horizon(6.5%).This explained the difference in soil K sat values.This study showed that Bt horizon had limited water transport capability, forcing part of the infiltrated rainwater as interflow through the OA and AB horizons.Thus, the topsoil responded quickly to rainfall events, causing frequent cycles of saturation and aeration of soil pores.     

Mating type ratios and pathogenicity in Diplodia shoot blight fungi populations: Comparative analysis

Diplodia sapinea and Diplodia scrobiculata are opportunistic pathogens of Pinus species. Several studies about taxonomy, impact and epidemiology of these fungi have been conducted in previous years, which have provided useful information and have raised new issues. These diseases produce a considerable impact on plantations resulting in significant economic losses. The main aims of this study are to increase the knowledge of the potential of genetic exchange and the relative aggressiveness of these organisms that can persist in healthy tissues of asymptomatic trees. A collection of 250 isolates among which are 149 strains collected from Pinus radiata plantations in Basque Country (Spain) and 101 strains from different countries was included in this work. Mating type ratios were analysed and compared using the structure of the MAT locus (MAT1‐1‐1 and MAT1‐2‐1). Inoculations of Pinus radiata seedlings were performed in a biosafety greenhouse (P2) to confirm pathogenicity of isolates and compare their aggressiveness. The frequency of occurrence of both idiomorphs of D. sapinea in Basque Country isolates was close to 1:1, however, for collection of isolates of this fungus from around the world, the ratio was 1:2. Furthermore, the spatial distribution of the two mating types in the Basque Country was random. Despite no detection of a sexual state, these results could suggest sexual reproduction behaviour. The pathogenicity of all strains in the collection was confirmed. Although aggressiveness (in terms of lesion lengths resulting from inoculation) varied greatly, no statistically significant effects of MAT type or pathogen species were detected.