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2.
Heuer C Wangler A Schukken YH Noordhuizen JP 《Veterinary journal (London, England : 1997)》2001,161(3):314-321
The objective of this study was to relate acetone in milk with cow and management factors in one low producing dairy herd (5260 kg milk per 305-day lactation). Milk acetone was measured in regular monthly milk samples one to three times within 100 days of lactation in 4433 lactations (2639 cows, 7800 measurements) from one herd over a period of 32 months (1988-91). Associations between milk acetone and cow factors and surrogate measures of management were evaluated by variance components of multiple fixed effect models. Lactation stage, calendar month of study, production groups and milk yield were strong, and percentage milk fat and parity were weak predictors of milk acetone. There was a trend of increasing body weight loss from the first to the second month of lactation with increasing milk acetone level. A substantial increase in milk production in 1991 was accompanied by an almost twofold rise in milk acetone. It was concluded that environmental parameters had strong relationships with milk acetone even in this low-producing herd. 相似文献
3.
AIMS: To assess the sensitivity and specificity of a bulk tank milk (BTM) antibody enzyme-linked immunosorbent assay (ELISA) to detect likely infection of a dairy herd with bovine vi- ral diarrhoea virus (BVDV). The ELISA was subsequently used to estimate the prevalence of likely infected herds in parts of the North Island of New Zealand. METHODS: BTM samples from 724 randomly selected dairy herds in the Waikato, Bay of Plenty and Northland regions of New Zealand were tested for BVDV antibodies. From this group, 20 herds were again randomly selected from each of the quartiles of the ELISA percentage inhibition (%INH) result. From each participant herd, serum from 15 randomly selected calves aged 6–18 months and 15 cows was collected and tested using an indirect blocking ELISA for BVDV antibodies. RESULTS: Among serum results from calves from 50 herds available for analysis, 34 (68%) herds were classified as likely non-infected (0-3 seropositive among 15 calves) and 16 (32%) as likely infected (5–15 seropositive among 15 calves). Receiver- operator characteristic (ROC) analysis identified an optimal cut-off for BTM of 80%INH associated with 81% sensitivity and 91% specificity for likely herd infection. The prevalence of BVDV antibodies in cows within herds and %INH for BVDV in bulk milk were positively correlated (p<0.01). The association between bulk milk %INH and the prevalence of BVDV antibodies in calves was stronger than the same association in cows. Based on the threshold of 80%INH, the 95% confidence interval (CI) for prevalence of likely infection in the 724 herds in the Waikato, Bay of Plenty and Northland regions of New Zealand was 12–17%. Vaccination against BVDV was not significantly associated with the likely infection status of the herd based on prevalence of BVDV antibodies among calves. CONCLUSION: An ELISA test result for BVDV antibodies in BTM ≥80%INH can be used as a threshold to indicate the presence of likely infection with BVDV in dairy herds in New Zealand, with 81% sensitivity and 91% specificity. 相似文献
4.
Holger Brueck 《植物养料与土壤学杂志》2008,171(2):210-219
The worldwide increase of food demand and reduced sweet‐water availability in some important food‐producing regions raised interest in more efficient water use, which has become one of the central research topics in agriculture. Improved irrigation management and reduced bare‐soil evaporation have highest priority to increase agronomic water‐use efficiency (WUE). Compared to these technical (irrigation) and basic (crop production) management options, effects of nutrient management on WUE were less frequently considered. Twenty‐nine publications on nitrogen (N) effects on biomass WUE of container‐grown plants are considered in this review. Most of them indicate positive N effects on WUE, and relevance of N effects on intrinsic WUE and unproductive water and carbon loss is discussed. A plot of 90 published data of percent decreases of WUE and dry mass under variable N supply is presented. Extrapolation of biomass WUE from leaf measurements of intrinsic WUE is critically reviewed. The positive correlation between WUE and dry‐mass formation suggests that physiological rather than stomatal effects are more important in order to explain positive N effects on WUE. 相似文献
5.
In spite of their low concentrations in soil solutions, low–molecular weight organic substances (LMWOS) such as amino acids, sugars, and uronic acids play a major role in the cycles of C and N in soil. With respect to their low concentrations and to possible matrix interferences, their analysis in soil leachates is a challenging task. We established two HPLC (high‐performance liquid chromatography) methods for the parallel determination of amino acids and carbohydrates in soil leachates. The pre‐column derivatization of amino acids with an o‐phthaldialdehyde (OPA) mercaptoethanol solution yields quantitation limits between 0.03 and 0.44 µmol L–1 and SD values of <8.3% (n = 9). High‐performance anion‐exchange chromatography (HPAEC) on a Dionex CarboPac PA 20 column with a NaOH acetate gradient combined with pulsed amperometric detection (PAD) was used for the determination of carbohydrates. The calibration curves obtained for 11 carbohydrates showed excellent linearity over the concentration range from 0.02 to 50.0 mg L–1. Recovery studies revealed good results for all analytes (89%–108%). Interferences from Hg(II) salts and chloroform used for stabilization of the leachates did not occur with both chromatographic methods. The optimized method was successfully used for quantitative determinations of amino acids and carbohydrates in soil leachates. 相似文献
6.
Using the soil gas radon as an indicator for ground contamination by non-aqueous phase-liquids 总被引:1,自引:0,他引:1
Michael Schubert Klaus Freyer Hans-Christian Treutler Holger Weiß 《Journal of Soils and Sediments》2001,1(4):217-222
1 The Problem One of the major problems facing risk assessment at polluted industrial sites and military bases is subsurface contamination
by non-aqueous phase-liquids (NAPLs), since tracing the extent of a NAPL plume using conventional methods (drive point profiling)
is usually associated with difficulties. In an effort to trace subsurface contamination as precisely as possible, monitoring
points are placed in the area that might be affected by contaminants, and groundwater and soil samples are taken to the laboratory
for analysis. However, the final number of monitoring points is hardly ever sufficient for distinctive contamination mapping,
and this may ultimately result in an unsuitable remediation action being taken.
2 Objectives To obtain a more detailed image of a subsurface NAPL plume and, hence, to facilitate remediation measures that are best suited
for the site in question, a denser network of monitoring points is desirable. The aim of the investigation described in this
paper was therefore to develop a new detection method for subsurface NAPL contamination, which is based on an easily accessibleindicator for NAPLs rather than on the analysis of soil and groundwater samples taken at the site. Based on the good solubility of
radon in NAPLs, the idea was put forward that subsurface NAPL contamination should have an influence on the natural radon
concentration of the soil gas. Provided this effect is significant, it would be possible to carry out a straightforward radon
survey on an appropriate sampling grid covering the suspected site and thus enabling the NAPL contamination to be detected
by the localization of anomalous low radon concentrations in the soil. The overall aim of the investigation was to assess
the general suitability of the soil-gas radon concentration as an indirect tracer for NAPL contamination in the ground.
3 Methods The partitioning coefficient KNAPL/air is one of the most influential parameters governing the decrease of the radon concentration in the soil gas in the presence
of a subsurface NAPL contamination. Since NAPL mixtures such as gasoline, diesel fuel and paraffin are among the most important
NAPLs regarding remediation activities, laboratory experiments were performed to determine the radon-partitioning coefficient
for these three NAPL mixtures.
Field experiments were carried out as well. The aim of the field experiments was to test the use of the soil-gas radon concentration
as a tracer for NAPL contamination on-site. For the field experiments, each site was covered with a suitable grid of soil
gas sampling points. Finally, the lateral radon distribution pattern achieved on each of the sites was compared to the respective
findings of the earlier research performed by conventional means.
4 Results and Discussion The results of the laboratory experiments clearly show a very strong affinity of radon to the NAPL mixtures examined. The
partitioning coefficients achieved correspond to those published for pure NAPLs (Clever 1979) and are thus in the expected
range. The results of the field experiments showed that the minimum radon concentrations detected match the respective NAPL
plumes traced previously.
5 Conclusions Both the results of the lab experiments and the on-site findings demonstrate that the soil-gas radon concentration can be
used as an indicator for subsurface NAPL contamination. The investigation showed that NAPL-contaminated soil volumes give
rise to anomalous low soil-gas radon concentrations in the close vicinity of the contamination. The reason for this decrease
in the soil-gas radon concentration is the good solubility of radon in NAPLs, which enables the NAPLs to accumulate and ‘trap’
part of the radon available in the soil pores.
6 Recommendations and Outlook Further research is required into contamination with rather volatile NAPLs such as BTEX. Further research is also needed to
examine whether it is possible to not only localize a NAPL plume, but also to obtain some quantitative information about the
subsurface NAPL contamination. The authors also believe that additional investigations should be carried out to study the
ability of the method to not just localize a NAPL contamination, but also to monitor on-site, clean-up measures. 相似文献
7.
8.
Effects of grazing and rainfall variability on root and shoot decomposition in a semi-arid grassland
Marcus Giese Ying Zhi Gao Ying Zhao Qingmin Pan Shan Lin Stephan Peth Holger Brueck 《Applied soil ecology》2009,41(1):8-18
Overgrazing increasingly affects large areas of Inner Mongolian semi-arid grasslands. Consequences for ecosystem functions and, in particular, for the decomposition as a key process of ecosystem carbon (C) and nitrogen (N) cycling are still unclear.We studied the effects of grazing on shoot and root decomposition with the litter bag method in a long-term grazing exclosure (UG79), a moderate winter grazed (WG) and a long-term heavily grazed site (HG). We separated the effects of local environmental factors and litter quality as altered by grazing. Growing seasons of average and very low precipitation allowed us to study the effect of inter annual rainfall variability on decomposition.Grazing-induced differences in environmental factors of the three studied grassland sites had no effect on decay rates of shoot and root dry mass. Also differences in litter quality among the grazing sites were not reflected by root decomposition dynamics. The accelerated shoot decay at site HG could not clearly be linked to litter quality parameters. Shoot decay rates were more or less constant, even under very dry conditions. This indicates the possibility of photodegradation (solar UV-B radiation) to control aboveground decomposition in this semi-arid ecosystem. By selecting the best predictors of root decomposition from regression analysis, we found that soil water content was the best parameter explaining the dynamics.Net N immobilization was generally not detected during the decay process of shoot and root. It is likely, when root decomposition is strongly reduced in dry periods, shoot decomposition becomes relatively more important for nutrient cycling. A separate analysis of shoot and root decay dynamics is required in order to describe C and N cycling in this semi-arid grassland. The grazing impact on C and N fluxes through decomposition of plant material likely exhibits a strong interaction with seasonal rainfall pattern. 相似文献
9.
Jeanloz R Ahrens TJ Lally JS Nord GL Christie JM Heuer AH 《Science (New York, N.Y.)》1977,197(4302):457-459
Transmission electron microscope (TEM) observations of an experimentally shock-deformed single crystal of natural peridot, (Mg(0.88)Fe(0.12))(2)SiO(4), recovered from peak pressures of about 56 x 10(9) pascals revealed the presence of amorphous zones located within crystalline regions with a high density of tangled dislocations. This is the first reported observation of olivine glass. The shocked sample exhibits a wide variation in the degree of shock deformation on a small scale, and the glass appears to be intimately associated with the highest density of dislocations. This study suggests that olivine glass may be formed as a result of shock at pressures above about 50 to 55 x 10(9) pascals and that further TEM observations of naturally shocked olivines may demonstrate the presence of glass. 相似文献
10.