BKCa-Cav channel complexes mediate rapid and localized Ca2+-activated K+ signaling

Large-conductance calcium- and voltage-activated potassium channels (BKCa) are dually activated by membrane depolarization and elevation of cytosolic calcium ions (Ca2+). Under normal cellular conditions, BKCa channel activation requires Ca2+ concentrations that typically occur in close proximity to Ca2+ sources. We show that BKCa channels affinity-purified from rat brain are assembled into macromolecular complexes with the voltage-gated calcium channels Cav1.2 (L-type), Cav2.1 (P/Q-type), and Cav2.2 (N-type). Heterologously expressed BKCa-Cav complexes reconstitute a functional "Ca2+ nanodomain" where Ca2+ influx through the Cav channel activates BKCa in the physiological voltage range with submillisecond kinetics. Complex formation with distinct Cav channels enables BKCa-mediated membrane hyperpolarization that controls neuronal firing pattern and release of hormones and transmitters in the central nervous system.     

Spatial (Im)mobility and Accessibility in Ireland: Implications for Transport Policy

Recent social research that links people's position in society to their ability to access employment has shown the centrality of spatial mobility in the (re)production of patterns of inequality. This is particularly evident in regions where economic activity is unevenly distributed and concentrated in an urban centre and where daily travel patterns reflect a spatial segregation between places of work and residential areas. This paper presents a spatial analysis of accessibility to employment for Galway City and its environs, a predominantly rural region in the West of Ireland dominated by its urban centre. Travel‐to‐work data from the 2006 Census of Population of Ireland were used to present a comparison of district‐specific accessibility levels across three socio‐economic groups. Network analysis and Geographic Information System visualisation tools are used to map existing socio‐spatial topographies of (in)accessibility. This is done to test two contrasting sets of theoretical proposals in the social science literature regarding the relationship between spatial mobility and social status. Advocates of the first position conceptualise spatial mobility as a form of capital that helps to maintain many existing social hierarchies. This contrasts with the views of those who anticipate the dissolution of established social boundaries (“fluidification”) as a result of increased spatial mobility of people, goods, and ideas. It is argued that these contrasting positions are highly relevant to current transport policy debates, including discussions around the impacts of recession‐related cuts in transport infrastructure investment on patterns of accessibility. In addition, they encourage reflection on the impacts of sustainable transport initiatives on different social groups that are more or less mobility disadvantaged, a fact that has hitherto received little attention in policy research and practice.     

Experimental autologous substitute vascular graft for transplantation surgery

Vascular complications in liver transplantation are a major cause of graft failure and mortality. The aim of the study was to create autologous vascular graft without risk of rejection. Posterior rectus fascia sheath lined with peritoneum was used for iliac artery replacement in seven mongrel dogs. The patency was followed by palpation and Doppler ultrasound. The grafts were removed after one month. Five grafts remained patent. The Doppler showed good, relatively increased flow (median flow rate: 383 cm/sec) after one month in all of the cases. Slight increase in diameter was present in all cases. By microscopy the five patent grafts showed viable morphology, fibroblasts, smooth muscle cells and thin fibrin layer in the wall. The grafts were lined partially with a neoendothelial monolayer and a thin fibrin layer. In conclusion, this graft presents an acceptable patency rate and low thrombogenicity, and could be useful in transplantation. Further investigations are needed to study the effect of immunosuppression and rejection on long-term morphology and patency of the grafts.     

Identity: key to children's understanding of belief

Is knowledge structured and acquired as independent facts and concepts, as parcels of independent domains, or as domains that share conceptual abilities? For an answer, we looked at the development of two concepts, belief and identity. These concepts are not part of the same domain, but the application of both depends on the common ability to separate sense from reference. We show that both capacities become functional between the ages of 3 and 5 years, which provides empirical support for the contention that deep conceptual structures play an important role in cognitive development.     

Herd specific risk factors for Mycoplasma hyopneumoniae infections in suckling pigs at the age of weaning

Background

Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia mainly occurring in fattening pigs. It is assumed that horizontal transmission of the pathogen during nursery and growing phase starts with few suckling pigs vertically infected by the sow. The aim of the present study was the exploration of the herd prevalence of M. hyopneumoniae infections in suckling pigs followed by an investigation of various herd specific factors for their potential of influencing the occurrence of this pathogen at the age of weaning.

Results

In this cross-sectional study, 125 breeding herds were examined by taking nasal swabs from 20 suckling pigs in each herd. In total, 3.9% (98/2500) of all nasal swabs were tested positive for M. hyopneumoniae by real-time PCR. Piglets tested positive originated from 46 different herds resulting in an overall herd prevalence of 36.8% (46/125) for M. hyopneumoniae infection in pigs at the age of weaning. While the herds were epidemiologically characterized, the risk for demonstration of M. hyopneumoniae was significantly increased, when the number of purchased gilts per year was more than 120 (OR: 5.8), and when the number of farrowing pens per compartment was higher than 16 (OR: 3.3). In herds with a planned and segregated production, where groups of sows entered previously emptied farrowing units, the risk for demonstration of M. hyopneumoniae in piglets was higher in herds with two or four weeks between batches than in herds with one or three weeks between batches (OR: 2.7).

Conclusions

In this cross-sectional study, several risk factors could be identified enhancing the probability of breeding herds to raise suckling pigs already infected with M. hyopneumoniae at the time of weaning. Interestingly, some factors (farrowing rhythm, gilt acclimatisation issues) were overlapping with those also influencing the seroprevalences among sows or the transmission of the pathogen between older age groups. Taking the multifactorial character of enzootic pneumonia into account, the results of this study substantiate that a comprehensive herd specific prevention programme is a prerequisite to reduce transmission of and disease caused by M. hyopneumoniae.     

Effect of nitrogen species supply and mycorrhizal colonization on organosulfur and phenolic compounds in onions

The aim of the present study was to test whether variations in the root environment affect the content of health-related organosulfur compounds, total phenolic compounds, and flavonol glycoside concentrations in onions. For this purpose, greenhouse-grown onions ( Allium cepa L.) were either inoculated with a commercial arbuscular mycorrhizal inoculum or a sterile inoculum and were provided with two NH(4)(+):NO(3)(-) ratios as a nitrogen source. Onion growth, arbuscular mycorrhizal colonization rate, sugars, and nutrient element concentrations were also quantified. The plant antioxidant activity and quercetin monoglucoside and organosulfur compound concentrations increased with dominant nitrate supply. Furthermore, mycorrhizal colonization increased the antioxidant activity and also concentrations of the major quercetin glucosides. The present study provides clear evidence that antioxidant activity, quercetin glycosides, and organosulfur compounds can be increased in sufficiently supplied onion plants by dominant nitrate supply or application of arbuscular mycorrhizal fungi. This was probably due to increased precursor production and induced defense mechanisms.     

Einfluss von Biostimulanzien und Lichtreflexionsfolie auf die Fruchtqualität und Farbentwicklung bei Apfel

The aim of this research was to improve inner and external fruit quality and fruit colouration using four-year-old apple ‘Braeburn, Hillwell’ trees on M9 (3?m × 1?m spacing) by employing reflective ground covers and biostimulants at Klein-Altendorf, nr. Bonn, Germany (50°N). Two reflective ground covers were spread on both sides of tree rows seven weeks before anticipated harvest and two biostimulants were applied two times – four and two weeks before harvest. Adjacent trees of the same row without reflective ground cover or without biostimulants served as control.Reflective ground covers significantly improved red colouration from 71°hue in the control to 49°hue with the ground cover Lumilys® and to 40°hue with Extenday®. The red colouration of the lower surface of fruits improved from 85°hue in the control to 52°hue with Lumilys® and 40°hue by Extenday®. Apple trees with Extenday® and Lumilys® produced 69% and 44% well-coloured fruit (with 75–100% fruit colouration) compared with 16–26% in the control.By contrast, an acceleration of ripening processes was not observed in plots with reflective ground covers compared with the control. There were no differences in sugar contents (°Brix) and fruit firmness (kg/cm²), but starch breakdown in fruit with reflective mulch was significantly delayed compared with the control resulting in retarded maturation.An economic analysis based on material costs and lifespan – excluding the labour costs for spreading and removing the ground covers – showed an economic advantage, if these mulches are used more than once a year.Both biostimulants showed neither effects on inner and external fruit quality nor on fruit colouration. Fruits with Wuxal®ATRiun or Sunred® did not improve the percentage of well-coloured fruits (75%–100% fruit surface with red colouration) compared with the untreated control. Similarly, inner and external fruit quality showed no significant differences in fruit firmness, sugar content and starch degradation. An economic analysis based on chemicals’ costs – excluding the labour costs for applying the biostimulants – resulted in no economic advantage. The chlorophyll – based ‘Index of Absorption Difference’ (IAD; measured non-destructively by the DA-meter) in some cases correlated positively with the Streif (maturation) index based on destructive assessment fruit firmness, sugar content and starch breakdown in an ‘ART system’.     

Influence of nitrogen forms and mycorrhizal colonization on growth and composition of Chinese bunching onion

In recent years, interest has grown in cultivating Allium species with enhanced health benefits and/or distinct flavor. Concentrations of phytochemicals determining these desired characteristics may be influenced by nitrogen forms (ammonium or nitrate) and arbuscular mycorrhizal (AM) fungi. We examined these relations with the test plant bunching onion (Allium fistulosum L.). Three different ammonium‐to‐nitrate (NH : NO ) ratios were supplied in combination with or without inoculation with an AM fungus (Glomus mosseae). The plants were evaluated for dry weight, leaf number, and content of nutrients (N, NO , P, S), sugars (glucose, fructose, and sucrose), and organosulfur compounds (measured as pyruvic acid). The experiment was carried out under controlled conditions in a greenhouse. Plants were grown on perlite amended twice a day with nutrient solution. In nonmycorrhizal plants, the application of nutrient solution with predominant NO or NH₄NO₃ as N source supported adequate growth of Allium fistulosum while predominant NH supply resulted in decreased growth and occurrence of wilting symptoms. Mycorrhizal inoculation significantly increased dry weight and leaf number of predominantly NH ‐fed or NH₄NO₃‐fed plants. While shoot P concentration increased with higher NH supply, shoot N concentration increased in predominantly NH ‐fed plants only. Nitrogen form and AM colonization had little effect on shoot S or sugar concentrations. The total content in organosulfur compounds was significantly affected by both, N form and AM colonization. The optimal growth condition for a high formation of organosulfur compounds in this experiment was a nutrient solution with predominant NO supply, but when supported by AM fungi, Allium fistulosum produced similar amounts of pyruvic acid in NH₄NO₃‐fed plants.     

Atypical myopathy in 2 Bactrian camels

Atypical myopathy (AM) is an acute seasonal rhabdomyolysis seen primarily in equids, caused by the ingestion of sycamore maple samaras containing hypoglycin A (HGA) and methylenecyclopropyl-glycine (MCPG). Toxic metabolites inhibit acyl-CoA dehydrogenases and enoyl-CoA hydratases, causing selective hyaline degeneration of type I muscle fibers. Two zoo-kept Bactrian camels (Camelus bactrianus) with a fatal course of AM had sudden onset of muscle pain and weakness, recumbency, and dysphagia, accompanied by increased serum creatine kinase activity and detection in serum of HGA, MCPG, and metabolites. Medical treatment was ineffective. At postmortem examination, sycamore maple tree material was found within the first gastric compartment of the 2-y-old gelding. Although musculature was macroscopically normal, histologically, monophasic hyaline degeneration was marked within type I fibers of intercostal and hypoglossal muscles of the gelding, and in neck, tongue, and masticatory muscles of the cow. The ingestion of sycamore maple material can cause AM in Bactrian camels, and trees of the Sapindaceae family should be avoided in enclosures.