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Identification of genic SSRs in jute (Corchorus capsularis,Malvaceae) and development of markers for phenylpropanoid biosynthesis genes and regulatory genes
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The advancement in functional genomics, such as DNA microarrays along with the genome availability of important pathogens as well as of human and livestock species has allowed scientists to study the expression of thousands of genes in a single step. In the past decade, DNA arrays have been employed to study infectious processes of pathogens, in diagnostics, and to study host-pathogen interactions. The generation of enormous data sets by microarray experiments also stimulated the growth of a new generation of analytical software. The information provided by microarray experiments has been useful in generating new hypotheses for future research. The concept of DNA array technology has been utilized in the development of novel diagnostic methods. This review highlights the application of microarrays in the field of veterinary research. 相似文献
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P. S. Mehta S. N. Ojha K. S. Negi S. K. Verma A. Rayal R. K. Tyagi 《Genetic Resources and Crop Evolution》2014,61(7):1279-1294
Rice (Oryza sativa L.) is one of the three major food crops of the world and India is the largest rice growing country accounting for about one-third of the world average under the crop. Rice is being cultivated in Northern India since ca 2000 BC as evident from archaeological studies. A total of 103 traditional varieties of rice collected from interior parts, during the study period. The present study was designed with the aim to document the rice diversity and its socio-economic and cultural importance for local farming communities of Garhwal Himalaya in Central Himalayan Region. For collection of information 324 households from 116 villages in the study area were sampled. The study revealed different agro-eco-niches of the study area, traditional processing pathways, socio-economic and cultural development, diversity available, factors responsible for erosion/replacement of diversity, aesthetic values and specialized food quality of local rice varieties etc. The number of rice varieties grown and available for cultivation in ten valleys of Garhwal Himalaya were documented so as these can be utilized and conserved for research and development in the future. 相似文献
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McEwen AS Ojha L Dundas CM Mattson SS Byrne S Wray JJ Cull SC Murchie SL Thomas N Gulick VC 《Science (New York, N.Y.)》2011,333(6043):740-743
Water probably flowed across ancient Mars, but whether it ever exists as a liquid on the surface today remains debatable. Recurring slope lineae (RSL) are narrow (0.5 to 5 meters), relatively dark markings on steep (25° to 40°) slopes; repeat images from the Mars Reconnaissance Orbiter High Resolution Imaging Science Experiment show them to appear and incrementally grow during warm seasons and fade in cold seasons. They extend downslope from bedrock outcrops, often associated with small channels, and hundreds of them form in some rare locations. RSL appear and lengthen in the late southern spring and summer from 48°S to 32°S latitudes favoring equator-facing slopes, which are times and places with peak surface temperatures from ~250 to 300 kelvin. Liquid brines near the surface might explain this activity, but the exact mechanism and source of water are not understood. 相似文献
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Prevalence of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis in representative Ontario swine herds 总被引:2,自引:0,他引:2
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Janet I. MacInnes Marcelo Gottschalk Abdul G. Lone Devon S. Metcalf Shivani Ojha Thomas Rosendal Sheila B. Watson Robert M. Friendship 《Canadian journal of veterinary research》2008,72(3):242-248
Tonsillar and nasal swabs were collected from weanling pigs in 50 representative Ontario swine herds and tested for the presence of 5 important bacterial upper respiratory tract pathogens. All but 1 herd (2%) tested positive for Streptococcus suis by polymerase chain reaction (PCR); 48% of herds were S. suis serovar 2, 1/2 positive. In all but 2 herds there was evidence of Haemophilus parasuis infection. In contrast, toxigenic strains of Pasteurella multocida were detected by a P. multocida--enzyme-linked immunosorbant assay (PMT-ELISA) in only one herd. Seventy-eight percent of the herds were diagnosed positive for Actinobacillus pleuropneumoniae by apxIV PCR. Sera from finishing pigs on the same farms were also collected and tested by ELISA for the presence of A. pleuropneumoniae antibodies. Seventy percent of the herds tested had evidence of antibodies to A. pleuropneumoniae including serovars 1-9-11 (2%), 2 (4%), 3-6-8-15 (15%), 5 (6%), 4-7 (26%), and 12 (17%). This likely represents a shift from previous years when infection with A. pleuropneumoniae serovars 1, 5, and 7 predominated. At least 16% and possibly as many as 94% of the herds tested were Actinobacillus suis positive; only 3 of the 50 herds were both A. pleuropneumoniae and A. suis negative as judged by the absence of a positive PCR test for apxII. Taken together, these data suggest that over the past 10 years, there has been a shift in the presence of pathogenic bacteria carried by healthy Ontario swine with the virtual elimination of toxigenic strains of P. multocida and a move to less virulent A. pleuropneumoniae serovars. As well, there appears to be an increase in prevalence of S. suis serovar 2, 1/2, but this may be a reflection of the use of a more sensitive detection method. 相似文献
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J. C. Rana T. R. Sharma R. K. Tyagi R. K. Chahota N. K. Gautam Mohar Singh P. N. Sharma S. N. Ojha 《Euphytica》2015,205(2):441-457