Effect of phosphorus fertilizer application on the performance of maize/soybean intercrop in the southern Guinea savanna of Nigeria

Field experiments were conducted at the Teaching and Research Farm, Ladoke Akintola University of Technology, Ogbomoso, Nigeria in 2007 and 2008 to determine the effects of phosphorus fertilizer application on performance of intercropped maize and soybean. The experiments, arranged as a split plot in a randomized complete block design, replicated four times. A cropping system with sole maize, sole soybean and maize/soybean intercrop formed the main plot treatments while P rates with 0, 15 and 30 kg P₂O₅ ha^?1 were the subplot treatments. For both years, neither P fertilizer application nor cropping systems had a significant effect on maize grain yield. However, soybean grain yield was significantly higher (92.3% in 2007 and 44.5% in 2008) under sole cropping than under maize/soybean intercropping. On average, N fixed by soybean increased with the increase in P rate (from 51.8% without P to 60.5% with 30 P), but there was no significant difference in N fixed by sole soybean and soybean/maize intercrop. However, the interaction effect on N fixed between cropping systems and P rates was significant (P ≤ 0.05). N, P and K contents in maize grain were significantly higher (>100%) in intercropped maize than in sole maize. The cropping systems had no significant effect on post-harvest soil chemical characteristics. The land equivalent ratio was 1.52 in 2007 and 1.78 in 2008. The result shows that in utilizing legumes for N enrichment, the alleviation of P deficiency can enhance N₂-fixation by legumes. Furthermore, P replenishment in a maize/soybean intercrop may improve maize grain quality even though yield is not increased.     

Genetic divergence in two tropical maize composites after four cycles of reciprocal recurrent selection

Two tropical maize composites were subjected to four cycles of reciprocal recurrent selection to develop divergent inbred lines with good combining ability. This study was conducted to examine the extent of genetic diversity, changes in allele composition and genetic structure, of 100 randomly selected S₁ lines each from the original (C₀) and advanced (C₄) selection cycles of TZL COMP3 and TZL COMP4, genotyped using single nucleotide polymorphism (SNP) markers. Results revealed that the proportion of alleles at both low and high frequencies decreased from C₀ to C₄, whereas those at intermediate frequencies increased at C₄ in the two composites. More unique and other alleles were lost at C₄ in TZL COMP3 relative to those in TZL COMP4. The changes in different measures of genetic diversity were either small or negligible with selection in the two composites. The proportion of markers departing from Hardy–Weinberg equilibrium (HWE) decreased with selection, whereas the total number of pairs of markers in linkage disequilibrium increased with selection in the two composites. Examination of changes in population structures using a model‐based approach as well as cluster and multivariate analyses found a high degree of concordance in stratifying the 400 S₁ lines into four non‐overlapping groups corresponding to the two selection cycles each within the reciprocal composites. The observed molecular‐based divergence between cycles within the same composite and the clear differentiation between the complementary composites highlight the importance of reciprocal recurrent selection for preserving genetic diversity for long‐term selection. This increases the potential of the advanced selection cycles to sustain genetic gain in productivity of hybrids adapted to the savannas in West and Central Africa.     

A Marine λ-Oligocarrageenan Inhibits Migratory and Invasive Ability of MDA-MB-231 Human Breast Cancer Cells through Actions on Heparanase Metabolism and MMP-14/MMP-2 Axis

Sugar-based molecules such as heparins or natural heparan sulfate polysaccharides have been developed and widely studied for controlling heparanase (HPSE) enzymatic activity, a key player in extracellular matrix remodelling during cancer pathogenesis. However, non-enzymatic functions of HPSE have also been described in tumour mechanisms. Given their versatile properties, we hypothesized that sugar-based inhibitors may interfere with enzymatic but also non-enzymatic HPSE activities. In this work, we assessed the effects of an original marine λ-carrageenan derived oligosaccharide (λ-CO) we previously described, along with those of its native counterpart and heparins, on cell viability, proliferation, migration, and invasion of MDA-MB-231 breast cancer cells but also of sh-MDA-MB-231 cells, in which the expression of HPSE was selectively downregulated. We observed no cytotoxic and no anti-proliferative effects of our compounds but surprisingly λ-CO was the most efficient to reduce cell migration and invasion compared with heparins, and in a HPSE-dependent manner. We provided evidence that λ-CO tightly controlled a HPSE/MMP-14/MMP-2 axis, leading to reduced MMP-2 activity. Altogether, this study highlights λ-CO as a potent HPSE “modulator” capable of reducing not only the enzymatic activity of HPSE but also the functions controlled by the HPSE levels.     

PCR‐based identification of cacao black pod causal agents and identification of biological factors possibly contributing to Phytophthora megakarya's field dominance in West Africa

Among the Phytophthora species that cause black pod of cacao, P. megakarya is the most virulent, posing a serious threat to cacao production in Africa. Correct identification of the species causing the black pod and understanding the virulence factors involved are important for developing sustainable disease management strategies. A simple PCR‐based species identification method was developed using the species‐specific sequences in the ITS regions of the rRNA gene. A phylogenetic tree generated for 119 Phytophthora isolates, based on the 60S ribosomal protein L10 gene and rDNA sequence, verified the PCR‐based identification assay and showed high interspecific variation among the species causing black pod. Phytophthora megakarya isolates were uniformly virulent in an assay using susceptible cacao pod husks inoculated with zoospores, while the P. palmivora isolates showed greater divergence in virulence. The virulence of P. megakarya was associated with earlier production of sporangia and an accelerated induction of necrosis. While zoospore germ tubes of both species penetrated pods through stomata, only P. megakarya produced significant numbers of appressoria. A hypersensitive‐like response was observed when attached SCA‐6 pods were inoculated with P. palmivora. SCA‐6 pods became vulnerable to P. palmivora when wounded prior to zoospore inoculation. Phytophthora megakarya was more aggressive than P. palmivora on attached SCA‐6 pods, causing expanding necrotic lesions with or without wounding. Phytophthora megakarya is predominant in the Volta region of Ghana and it remains to be seen whether it can displace P. palmivora from cacao plantations of Ghana as it has in Nigeria and Cameroon.     

The present status of biological control of Xanthium (Compositae)

Abstract

The economic threshold level of the mustard aphid, Lipaphis erysimi (Kaltenbach) was determined on the radish seed crop, var Punjab Sufed. Spraying oxydemeton methyl at 300 g a.i./ ha, was monitored at arbitrary set aphid levels from 25 to 150 aphids/plant. The maximum cost benefit ratio (1: 13.1) was achieved at an aphid level of 50 per plant, requiring three sprays. Spraying in the middle of February was the most crucial, as delay of 7 days from this stage resulted in significant decreases in yield in the fixed spray schedule and at the 75‐aphid level.     

Effects of leguminous plant residues and NPK fertilizer application on the performance of yam (Dioscorea rotundata ‘c.v.’ ewuru) in south-western Nigeria

The effects of cultivating and incorporating residues of previous tropical kudzu (Pueraria phaseoloides) and soybean (Glycine max) with application of NPK fertilizer on yam performance were evaluated at the teaching and research farm, LAUTECH, Nigeria. There were nine treatments: incorporation of legume residues (5 t DM ha^?1), application of recommended fertilizer rate for yam (90–50–75 kg NPK ha^?1) in the zone or 50% of recommended rate (45–25–37.5 kg NPK ha^?1), alone and in combination with residues and a control without residues or fertilizer in a randomized complete block design. Cultivation of previous legumes reduced soil nematode population (>200%) compared with no legumes. For both years, application of Pueraria residues improved tuber yield by an average of 15.8% compared with control. Fertilizer application enhanced arbuscular mycorrhizal (AM) colonization of yam roots but AM colonization was lower (～50%) in plots where Pueraria residues were incorporated compared with other plots. Combined application of plant residues with fertilizer improved soil organic carbon, total N, exchangeable Ca and Mg compared with application of NPK fertilizer. From these results, it is concluded that half of the recommended NPK rate may be adequate and incorporation of residues with reduced NPK fertilizer application may be a sustainable soil fertility management option for continuous yam production.     

Immunoblotting study of the antigenic relationships among eight serogroups of Leptospira.

Seven strains of Leptospira interrogans belonging to seven different serogroups, and one strain of Leptospira biflexa were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with gradient gels and immunoblotting with hyperimmune rabbit sera raised against each strain. The molecular masses of the proteins were calculated with a polynomial regression model. The SDS-PAGE patterns of the L. interrogans strains were similar and characterized by 24 common bands. This profile was not found for L. biflexa. The immunoblots obtained either with the seven anti-L. interrogans sera or the anti-L. biflexa serum allowed a clear distinction between the two species. Taken as a whole, the L. interrogans strain patterns revealed by the seven anti-L. interrogans sera were similar, sharing eight common major bands. A serovar- or serogroup-specific antigenic zone, ranging from 21 to 26 kDa, was also identified.     

Soil physical properties of selected soil series in relation to compaction and erosion on farmers’ fields at Abeokuta,southwestern Nigeria

This study evaluated physical properties of selected soil series and their implications on the soil compaction and erosion in Abeokuta, southwestern Nigeria. Daily rainfall data (1999–2007) were collected to estimate the rainfall erosivity. Seven soil series (Iwo, Iseyin, Ekiti, Jago, Okemesi, Apomu, and Egbeda) were sampled from 0–15, 15–30, and 30–50 cm depths for particle size distribution, organic carbon, pH, upper plastic limit, and compactibility (Proctor test). Microtopographical changes along and across toposequences of two farmers’ fields cleared mechanically and manually, respectively, were monitored using the erosion pin method. Mean annual erosivity (EI30) was high (7646 MJ mm ha^?1 hr^?1). Particle size, organic carbon, and pH were similar (p ≥ 0.05), while upper plastic moisture was ≤?2% among different soil series. Soil-moisture density curves indicated a maximum bulk density of 1.77–1.99 g cm^?3 for a moisture range of 7.6–14%; while the soils were prone to compaction at low moisture content. Microtopographic changes were found between –2 and 0 cm and –8 and –2 cm on mechanically and manually cleared farmland, respectively. Spatial dependence showed that the soil erosion could be predicted within 5–8 m distance. To avoid erosion and compaction, soil water content should be less than 7.6% before the introduction of mechanical tillage.     

Development and characterization of five novel cell lines from snubnose pompano,Trachinotus blochii (Lacepede, 1801), and their application in gene expression and virological studies

Five novel permanent cell lines have been established from gill, heart, kidney, eye and fin of snubnose pompano, Trachinotus blochii. They were designated as snubnose pompano gill (SPG), snubnose pompano heart (SPH), snubnose pompano kidney (SPK), snubnose pompano eye (SPE) and snubnose pompano fin (SPF), respectively. All these cell lines were characterized and cryopreserved successfully at different passage levels. Cell lines were passaged every alternate day; SPG, SPH, SPK, SPE and SPF cell lines attained passage levels of 68, 74, 82, 79 and 106, respectively, since the initiation of their development in 2019. The cell lines grew well in Leibovitz's 15 medium containing 15% foetal bovine serum at 28°C. Immunophenotyping of the cell lines revealed the presence of fibronectin and pancytokeratin. No mycoplasma contamination was found. The transfection study revealed the gene expression efficiency of these cell lines by expressing the green fluorescent protein (GFP). The authentication on origin of cell lines from T. blochii was confirmed by amplification of species-specific mitochondrial cytochrome oxidase I gene. The results showed the susceptibility of these cell lines to fish nodavirus (FNV) and tilapia lake virus (TiLV) and resistance to cyprinid herpesvirus 2 (CyHV-2). The FNV infection in the cell lines was confirmed by RT-PCR, Western blot, ELISA and immunocytochemistry, while TiLV infection was confirmed by RT-PCR assay. These results revealed that these cell lines are suitable for virological and foreign gene expression studies.