In utero programming of sexually differentiated gonadotrophin releasing hormone (GnRH) secretion

It has long been recognised that steroids can have both organisational and activational effects on the reproductive neuroendocrine axis of many species, including the sheep. Specifically, if the ovine foetus is exposed to testosterone during a relatively short 'window' of in utero development (from approximately day 30-90 of a 147 day pregnancy) the neural mechanisms regulating gonadotrophin releasing hormone (GnRH) secretion become organised in a male-specific manner. In post-natal life the consequences of foetal androgen exposure are sexually differentiated responses of the GnRH neuronal network to activation by factors such as photoperiod and ovarian steroid hormones. Studies in the gonadectomized lamb have demonstrated that elevated concentrations of oestrogen (E) are unable to trigger a preovulatory-like GnRH surge in the male and the androgenized ewe lamb. Further, these animals have markedly reduced sensitivity to the inhibitory actions of progesterone on tonic GnRH release compared with normal ewes. The reasons for these abnormal steroid feedback mechanisms may reside in sexually dimorphic inputs to the GnRH neurone, including those from oestrogen-receptive neurones in the arcuate nucleus that synthetize the neuropeptide, neurokinin B (NKB). The consequences of in utero androgen exposure are reflected in a progressive and dramatic impairment of fertility in the ovary-intact ewe.     

High efficiency DNA-mediated transformation of primate cells

Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase or Tn5 aminoglycoside phosphotransferase dominant selectable markers. Human HeLa and SV40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). CV-1, an African green monkey kidney cell line, could be stably transformed with the exceptionally high frequency of 6 X 10(-2) (6 percent).     

Improved management of Vertisols in the semiarid tropics for increased productivity and soil carbon sequestration

Abstract. This study was undertaken to test the hypothesis that an improved system of catchment management in combination with appropriate cropping practices can sustain increased crop production and improve soil quality of Vertisols, compared with prevailing traditional farming practices. Initiated in 1976, the improved system consisted of integrated land management to conserve soil and water, with excess rainwater being removed in a controlled manner. This was combined with improved crop rotation (legume based) and integrated nutrient management. In the traditional system, sorghum or chickpea was grown in the post-rainy season with organic fertilizers, and in the rainy season the field was maintained as a cultivated fallow. The average grain yield of the improved system over 24 years was 4.7 t ha⁻¹ yr⁻¹, nearly a five-fold increase over the traditional system (about 1 t ha⁻¹ yr⁻¹). There was also evidence of increased organic C, total N and P, available N, P and K, microbial biomass C and N in the soil of the improved system. A positive relationship between soil available P and soil organic C suggested that application of P to Vertisols increased carbon sequestration by 7.4 t C ha⁻¹ and, in turn, the productivity of the legume-based system, thus ultimately enhancing soil quality.     

Pharmacokinetics and relative bioavailability of tiamulin in broiler chicken as influenced by different routes of administration

The bioavailability and pharmacokinetic disposition of tiamulin in broiler chicken were investigated after administration through the crop, drinking water, and feed at 40 mg/kg body weight. Residues of tiamulin in tissues of broiler chicken were also assessed. Plasma and tissue concentrations of tiamulin were analyzed by reverse‐phase high‐performance liquid chromatography (HPLC) method. Plasma concentration–time data were described by the non‐compartmental model for all three routes, and pharmacokinetic parameters were calculated. There were no significant differences (p > 0.05) in pharmacokinetic parameters and mean plasma concentrations of tiamulin between three routes tested (crop, water, and feed), indicating equal efficacy. Tiamulin residues in edible tissues (muscles, skin, and fat) were lower than the advocated maximum residue limit (MRL of 0.1 µg/g and that of liver was 1 µg/g) on the 3rd day. No traces were found on the 5th day after drug administration. This indicated that the withdrawal period (less than 5 days) is very short, which makes it safer. This study shows that tiamulin can be used with equal efficacy through all routes of administration in broiler chicken (crop, water, and feed).     

Development of chitosan conjugated DNA vaccine against nodavirus in Macrobrachium rosenbergii (De Man, 1879)

The protective efficacy of a DNA construct containing extra small virus antisense (XSVAS) gene of nodavirus encapsulated with chitosan nanoparticles (NPs) was investigated in giant freshwater prawn Macrobrachium rosenbergii (De Man, 1879). The delivery was carried out using oral and immersion methods. A plasmid concentration of 100 ng μL^?1 when conjugated with chitosan NPs was found to be more effective in increasing the survivability of the infected prawn. The particle mean size, zeta potential and loading efficiency percentage were 297 nm, 27 mV and 85%, respectively. The ability of the chitosan to form a complex with the plasmid was studied by agarose gel electrophoresis. The NPs were characterized by atomic force microscopy (AFM). Persistence study showed the presence of the DNA construct up to 30th day post‐treatment. The oral treatment was found to be better than the immersion treatment for delivery of the chitosan‐conjugated DNA construct. This is probably the first report on the delivery of nanoconjugated DNA construct in M. rosenbergii, against nodavirus.     

Classifying soils at the ultimate stage of weathering in the tropics

Oxisols cover ≈ 23% of the land surface in the tropics and are utilized extensively for agricultural purposes in the tropical countries. Under the variable input types of agricultural systems practiced locally, some of these soils still appear to have problems in terms of proper soil classification and subsequently hinder attempts to implement sustainable agro‐management protocols. The definition for Oxisols in Soil Survey Staff (1999) indicates that additional input is still required to refine the definition in order to resolve some of the outstanding classification problems. Therefore, the objective of this study is to examine the properties of some Oxisols and closely related soils in order to evaluate the classification of these soils. Soils from Brazil, several countries in Africa, and Malaysia were used in this study. Field observations provided the first indication that some of the presently classified kandi‐Alfisols and kandi‐Ultisols were closer to Oxisols in terms of their properties. Water‐retention differences and apparent CEC of the subsurface horizons also supported this idea. The types of extractable Fe oxides and external specific surface areas of the clay fractions showed that many kandic horizons have surface properties that are similar to the oxic horizons. Micromorphology indicated that the genetic transition from the argillic to the oxic involves a diminishing expression of the argillic. Properties, such as CEC, become dominant. The kandic horizon is therefore inferred as a transition to the oxic horizon. It is proposed that the Oxisols be keyed out based only on the presence of an oxic horizon and an iso–soil temperature regime. The presence of a kandic horizon will be reflected at lower levels in Oxisols. The Oxisols will now be exclusive to the intertropical belt with an iso–soil temperature regime. The geographic extend of the Oxisols would increase and that of kandi‐Alfisols and Ultisols would decrease. A few kandi‐Alfisols and Ultisols in the intertropical area will have low CEC which would fail the weatherable mineral contents. The kandic subgroups of some Alfisols and Ultisols will be transitional between the low (< 16 cmol_c [kg clay]^–1)‐ and high (> 24 cmol_c [kg clay]^–1)‐activity clay soils. The proposed changes to classification will contribute to a better differentiation of the landscape units in the field. Testing of the proposed classification on some Malaysian soils showed that the new definition for Oxisols provides a better basis for the classification of the local soils and the development of meaningful soil‐management groups for plantations.     

The weathering of biotite in a profile on gneiss in Malaysia

Biotite and its pseudomorphs were studied in a deep profile on gneiss from Malaysia. Alteration of biotite to halloysite takes place within a few centimeters of the fresh rock (at 18.5 m depth) in this humid tropical environment. The halloysite crystals are arranged parallel to the planar surface of the biotite (SEM studies) in the interlamellar spaces. There are zones in the profile where iron has accumulated and some of the iron crystallises as goethite on the edges and surfaces of the flakes. Once this coating has formed, further alteration is retarded as is shown by the fact that pseudomorphs with the coating contain both halloysite and vermiculite though the environment where they are present (position in the profile) is acidic and leaching.