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Bushey Joseph T. Ebbs Stephen D. Dzombak David A. 《Water, air, and soil pollution》2004,157(1-4):281-293
Water, Air, &; Soil Pollution - A method for free cyanide and strongly-complexed cyanide measurement within plant tissue was developed to study uptake and movement of cyanide species separately... 相似文献
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The β‐cyanoalanine pathway in plants detoxifies cyanide by assimilating this metabolic poison. Given the possibility that cyanide in soil could serve as an alternate source of nitrogen for plant nutrition, this study investigated whether nitrogen deprivation of wheat seedlings altered the activity of the first enzyme of the pathway (β‐cyanoalanine synthase) or asparaginase. The results suggest that ambient, nontoxic concentrations of soil cyanide may serve as an alternate source of nitrogen for plants under nitrogen‐limiting conditions. 相似文献
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Corbit RM Ferreira JF Ebbs SD Murphy LL 《Journal of agricultural and food chemistry》2005,53(26):9867-9873
Four methods were tested for extraction and recovery of six major ginsenosides (Rb1, Rb2, Rc, Rd, Re, and Rg1) found in roots of American ginseng (Panax quinquefolius): method A, sonication in 100% methanol (MeOH) at room temperature (rt); method B, sonication in 70% aqueous MeOH at rt; method C, water extraction (90 degrees C) with gentle agitation; and method D, refluxing (60 degrees C) in 100% MeOH. After 0.5-1 h, the samples were filtered and analyzed by high-performance liquid chromatography (HPLC)-UV. A second extraction by methods C and D was done, but 85-90% of ginsenosides were obtained during the first extraction. Lyophilization of extracts did not influence ginsenoside recovery. Method D resulted in the highest significant recoveries of all ginsenosides, except Rg1. Method C was the next most effective method, while method A resulted in the lowest ginsenoside recoveries. Method B led to similar recoveries as method C. All methods used one filtration step, omitted time-consuming cleanup, but maintained clear peak resolution by HPLC, and can be used for quantitative screening of ginsenosides from roots and commercial ginseng preparations. 相似文献
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