Hepatic lipidosis: Liver characteristics and acute phase proteins in affected turkeys

The hepatic lipidosis (HL) in fattening turkeys is a disease has been known for a long time, but the cause and pathogenesis is still not clarified. A recent study reported unexplained high levels of iron in liver tissue of fattening turkeys suffering from HL. In this study, the iron status, possible infectious or inflammatory influences in form of an acute phase reaction and the analysis of fatty acid pattern in liver tissue of turkeys affected by HL were examined. Three cases of HL on three different fattening turkey farms were investigated during the outbreak of the disease. Clinically affected and non-affected animals were subjected to a pathological examination, where the diagnosis HL or non-affected was made. In total, 70 birds were examined (40 with HL, 30 without HL) and blood and liver samples were taken. Additionally, samples from 15 slaughtered birds were taken as a further control group. In liver tissue, the iron content and the content of long-chain fatty acids were determined; in blood samples, ferritin and transferrin were measured. The iron content in liver tissue was more than three times higher for animals with HL than among non-affected animals and the control group. The transferrin levels were lowest for animals with HL, highest in the control group and in between for non-affected animals. The fatty acid pattern in liver tissue of affected animals indicated a shift from polyunsaturated fatty acids to saturated fatty acids and monounsaturated fatty acids compared to the control group and the non-affected animals. Overall, the non-affected animals of a flock affected by HL were similar to the healthy animals of the abattoir. The low acute phase protein levels for animals with HL together with high iron contents could indicate a previous malnutrition/starvation period and/or severe liver damage for those animals suffering from HL.     

Habitat fragmentation in an urban environment: large and small fragments support different arthropod assemblages

We investigated the effects of fragmentation due to urbanisation on the species composition and functional roles of ants, beetles, spiders, flies and wasps. The study was conducted in 21 fragments of heath and woodland in south-eastern Australia classed as either ‘small’ (? 4 km²) or ‘large’ (? 80 km²). Arthropods were pitfall-trapped and identified to family or genus and morphospecies and microhabitat characteristics were recorded. Large fragments did not support more species per unit area than small fragments for most arthropods, although there were more species of ants per sampling unit in small than large woodland fragments, mainly due to a higher frequency of generalist species in smaller fragments. Large and small habitat fragments contained different assemblages of spiders, wasps and ants, indicating that predators and parasitoids are affected more strongly than other trophic groups. Arthropod assemblages within larger fragments where grids were furthest apart were less similar than those within smaller fragments where grids were closer together in woodland, but not in heath. The responses of arthropods to fragmentation suggest that, in addition to effects of reduced area and proximity to the urban matrix, changes in fire regimes and the degradation of habitats resulting from urbanisation, may have a role in altering arthropod assemblages, particularly affecting those species belonging to higher trophic levels. Management goals for urban remnants should identify mechanisms for controlling fire and anthropogenic disturbance such that they closely resemble the levels of these factors in larger fragments.     

CO2-stunning of swine for slaughter from the anesthesiological viewpoint

For investigations of CO2-stunning of feeder- and slaughter-pigs parameters of behaviour, blood-gas-analyses and electroencephalograms were chosen. The following results were obtained: 1. Blood-gas-analyses proved that the CO2-stunning does not produce unconsciousness due to a lack of oxygen. 2. The criterias of general anaesthesia: unconsciousness, muscle-relaxation and analgesia with total reversibility could be confirmed. 3. The violent convulsive symptoms were evaluated as reactions identical with the stage II of GUEDEL's scheme of anaesthesia. 4. Muscular agitation, which sometimes appeared a few seconds before the stage of excitation, was judged to belong either to the start of the excitation phase or to the end of Guedel's stage of analgesia, during which the sensitivity is decreased. Neither study of behavior nor objective measurements showed, during the first 10 to 20 seconds of exposure to the CO2, any sign of pain or suffering related to the Act for Prevention of Cruelty to Animals, and accordingly such suffering should not be ascribed to the CO2 stunning method.