Mapping of quantitative trait loci for floral scent compounds in cowpea (Vigna unguiculata L.)

Floral scent is a very important trait in plant evolution. Currently, little is known about the inheritance of floral scent in cowpea (Vigna unguiculata L.) or changes that might have occurred during its domestication. Therefore, we analysed scent volatiles and molecular markers in a population of 159 F₇ recombinant inbred lines derived from a cross of a domesticated blackeye cowpea cultivar, ‘524B’ and a wild accession ‘219‐01’. Using gas chromatography‐mass spectrometry (GC–MS) 23 volatile compounds were identified that fall into five general functional categories. Twenty‐two of the compounds displayed quantitative variation in the progeny, and a total of 63 QTLs influencing the amounts of these volatiles were mapped onto the cowpea genetic marker map. Although QTLs for volatile compounds putatively involved in cowpea flower scent were found on 9 of the 11 cowpea chromosomes, they were not evenly distributed with QTLs mainly clustered on LGs 1, LGs 2 and LG 4. Our results serve as a starting point for both more detailed analyses of complex scent biosynthetic pathways and the development of markers for marker‐assisted breeding of scented rose varieties.     

Factors Affecting Small-Scale Farmers’ Land Allocation and Tree Density Decisions in an <Emphasis Type="Italic">Acacia decurrens</Emphasis>-Based <Emphasis Type="Italic">taungya</Emphasis> System in Fagita Lekoma District,North-Western Ethiopia

The development of woodlots as an additional source of livelihood and as a land management option for small-scale farmers is a challenging issue in the cereal-based subsistence farming systems of north-western Ethiopia. There is a need to understand why and which factors determine the decisions of small-scale farmers to grow short-rotation woodlots on their land. Data used in this study were collected from a survey of 200 randomly selected households in the region. A Tobit regression model was used to determine predictor variables for farmers’ decisions to allocate land to planting Acacia decurrens (J.C. Wendl.) Willd. and at what density trees are planted on the respective plots. The most important motivations for planting A. decurrens were income, soil fertility management, and soil and water conservation. Having a male head of household, long distance to markets and plots being on marginal land, among other factors, increased the allocation of land to A. decurrens woodlots. Having a male head of household, access to credit and plots being on marginal land, among other factors, increased tree planting density. Age had a negative effect on both allocation of land to woodlots and tree density, whereas farm size had an inverted U-shaped relationship with both decisions. These results suggest that wider expansion of A. decurrens-based plantation systems could be achieved through improving extension, credit access and road infrastructure to connect small-scale farmers to markets and finance.     

Reservoir sedimentation and its mitigating strategies: a case study of Angereb reservoir (NW Ethiopia)

Purpose  

The Angereb dam in northwestern Ethiopia was commissioned in 1997 to serve as a domestic water supply for 25 years. However, its sustainability is being threatened by rapid sedimentation. The overall objective of this study was to understand reservoir sedimentation in this tropical highland watershed and to propose its mitigating strategies that would contribute to the improved planning and management of reservoirs in similar regions.     

Efficiency of soil and water conservation practices in different agro-ecological environments in the Upper Blue Nile Basin of Ethiopia

In developing countries such as Ethiopia, research to develop and promote soil and water conservation practices rarely addressed regional diversity. Using a water-balance approach in this study, we used runoff plots from three sites, each representing a different agro-ecological environment, e.g., high, mid and low in both elevation and rainfall, in the Upper Blue Nile Basin of Ethiopia to examine the runoff response and runoff conservation efficiency of a range of different soil and water conservation measures and their impacts on soil moisture. The plots at each site represented common land use types(cultivated vs. non-agricultural land use types) and slopes(gentle and steep). Seasonal runoff from control plots in the highlands ranged 214–560 versus 253–475 mm at midlands and 119–200 mm at lowlands. The three soil and water conservation techniques applied in cultivated land increased runoff conservation efficiency by 32% to 51%, depending on the site. At the moist subtropical site in a highland region, soil and water conservation increased soil moisture enough to potentially cause waterlogging, which was absent at the lowrainfall sites. Soil bunds combined with Vetiveria zizanioides grass in cultivated land and short trenches in grassland conserved the most runoff(51% and 55%, respectively). Runoff responses showed high spatial variation within and between land use types, causing high variation in soil and water conservation efficiency. Our results highlight the need to understand the role of the agro-ecological environment in the success of soil and water conservation measures to control runoff and hydrological dynamics. This understanding will support policy development to promote the adoption of suitable techniques that can be tested at other locations with similar soil, climatic, and topographic conditions.     

Is bigger better? Apidae (Xylocopinae), megachilidae and cowpea (Vigna unguiculata) pollination

Cowpea is an important grain legume crop in Africa. Cowpea flowers are capable of self-fertilization, which might be the most frequent type of reproduction. Nevertheless, the rate of cross-pollination could vary between 1% and 10%, depending on the populations of bumblebees or domestic bees that are present, the climate and the cultivar. The aim of this study was to identify and assess the efficiency rate of the pollinators of the wild cowpea, Vigna unguiculata subsp. unguiculata var. spontanea, the progenitor of the domesticated cowpea Vigna unguiculata subsp. unguiculata. Pollination study was conducted by observing patches of flowers (average 10–20/m²) from dawn to midday, approximately 05:00–12:30 hr for three years. All insects visiting the flowers were noted and identified to species level, where possible, and their foraging behaviour was monitored. Pollen grain deposit and pod set were estimated using single bee visits on plants with strictly outcrossing flowers. Major flower visitors were large bees of the family Megachilidae and the genus Xylocopa (Apidae, Xylocopinae). Xylocopa caffra (Linnaeus) was the most abundant bee species, accounting for 59% of total bees observed and 58% of total flower visited. Pod set was higher in visits by megachilid bees compared to Xylocopa bees. Within Xylocopa, there is a good correlation between size and efficiency (r = .95, p = .0477), the larger insects being more efficient. Honeybees, small bees (Ceratina and Nomia species) and Lepidopterans also visited flowers but without any effect on pollination. Cowpea pollination system is specialized, and pollinators are restricted to Megachilidae and Xylocopa species. Variation in pollen load deposit and pod set reveals that pollination success depends on the insect species involved and their size. Megachilids are the most efficient pollinators, especially since they are able to promote outcrossing more efficiently than Xylocopa species.     

Serological survey of African horse sickness in selected districts of Jimma zone,Southwestern Ethiopia

A cross-sectional serological survey was undertaken in selected districts of different agro-ecology of Jimma zone (Dedo, Yebu, Seka, Serbo, and Jimma town) from November 2009 to February 2010 to determine the seroprevalence of African horse sickness virus and associated risk factors of the disease. Two hundred seventy-four equids (189 horses, 43 mules, and 47 donkeys) with a history of non-vaccination for at least 2 years were selected randomly from the above areas. Sera samples were collected and assayed for the presence of specific antibody against African horse sickness virus using blocking ELISA. An overall seroprevalence of 89 (32.5%) was found and it was 24 (51.1%) for donkeys, 13 (30.2%) for mules, and 52(28.3%) for horses. Seroprevalence was significantly (X ² = 11.05, P < 0.05) different among the different species of equids. Seroprevalence was also significantly (X ² = 11.43, P < 0.05) different among the different agro-ecological areas being higher in highlands 47 (40.5%) followed by midland 30 (34.5%) and lowland 12 (16.9%). Age and sex were not significantly (X ² = 3.15, P > 0.05 and X ² = 3.38, P > 0.05, respectively) associated with seroprevalence of AHSV. The present study showed that African horse sickness (AHS) is highly prevalent disease for the horses followed by mules and then donkeys in Jimma zone explained by lower seroconversion rate. Therefore, control strategy against AHS should target at high risk species of all age and sex in their locality in the initial stage for better containment of the disease.     

Molecular mapping of QTLs for domestication-related traits in cowpea (V. unguiculata (L.) Walp.)

Understanding the genetic basis underlying domestication-related traits (DRTs) of cowpea (Vigna unguiculata (L.) Walp.) is important since the genome has experienced divergent domestication and in addition it is also useful to utilize the wild germplasm efficiently for improving different traits of the cultivated cowpea. Quantitative trait loci (QTLs) for DRTs were identified in a population of 159 F₇ recombinant inbred lines derived from a cross between a domesticated cowpea (V. unguiculata (L.) Walp.) variety, 524B, and a wild accession, 219. Using the constructed linkage map, QTLs for 10 DRTs were analysed and mapped. QTLs for seed, pod and flower related traits were detected. Subsequently, QTL for ovule number was also identified. To our knowledge, this is the first time a QTL for this trait has been observed. QTLs for DRTs show co-localization on three linkage groups and pleiotropy or close linkage of genes for the traits is suggested in these chromosome regions. The information gained in this study can be used for marker-assisted selection of domestication-related QTLs in cowpea and enhance understanding of domestication in the genus Vigna.