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1.
Equine polymorphonuclear (PMN) and mononuclear (MN) leucocytes were separated on Percoll gradients and used to study the chemoattractant properties of the polar ether-linked phospholipid, platelet activating factor (PAF). Six concentrations of PAF ranging from 1 ng/ml to 100 g/ml were studied in each of two in vitro assay systems, the agarose microdroplet and a microfilter technique. Very significant (p<0.01) increases in the movement of both PMN and MN cells were obtained with most concentrations of PAF. In two instances there was no apparent concentration-response relationship, although the action of PAF was approximately bell-shaped in two others. The possible significance of these findings for equine inflammatory conditions is discussed. 相似文献
2.
Feline calicivirus (FCV) is an important and highly prevalent pathogen of cats. It belongs to the family Caliciviridae which includes other significant pathogens of man and animals. As an RNA virus, high polymerase error rates convey upon FCV a high genome plasticity, and allow the virus to respond rapidly to environmental selection pressures. This makes the virus very adaptable and has important implications for clinical disease and its control. Being genetically diverse, FCV is associated with a range of clinical syndromes from inapparent infections to relatively mild oral and upper respiratory tract disease with or without acute lameness. More recently, highly virulent forms of the virus have emerged associated with a systemic infection that is frequently fatal. A proportion of FCV infected cats that recover from acute disease, remain persistently infected. In such cats, virus evolution is believed to help the virus to evade the host immune response. Such long-term carriers may only represent a minority of the feline population but are likely to be crucial to the epidemiology of the virus. Vaccination against FCV has been available for many years and has effectively reduced the incidence of clinical disease. However, the vaccines do not prevent infection and vaccinated cats can still become persistently infected. In addition, FCV strain variability means that not all strains are protected against equally. Much progress has been made in understanding the biology and pathogenesis of this important feline virus. Challenges for the future will necessarily focus on how to control the variability of this virus particularly in relation to emerging virulent strains and vaccination. 相似文献
3.
Feline herpesvirus (FHV-1; felid herpesvirus 1 (FeHV-1)) is an alphaherpesvirus of cats closely related to canine herpesvirus-1 and phocine herpesvirus-1. There is only one serotype of the virus and it is relatively homogenous genetically. FeHV-1 is an important cause of acute upper respiratory tract and ocular disease in cats. In addition, its role in more chronic ocular disease and skin lesions is increasingly being recognised. Epidemiologically, FeHV-1 behaves as a typical alphaherpesvirus whereby clinically recovered cats become latently infected carriers which undergo periodic episodes of virus reactivation, particularly after a stress. The primary site of latency is the trigeminal ganglion. Conventional inactivated and modified-live vaccines are available and protect reasonably well against disease but not infection, although viral shedding may be reduced. Genetically engineered vaccines have also been developed, both for FeHV-1 and as vector vaccines for other pathogens, but none is as yet marketed. 相似文献
4.
Movement and habitat use of the freshwater catfish (Tandanus tandanus) in a remnant floodplain wetland
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Wayne M. Koster David R. Dawson Pam Clunie Fern Hames John McKenzie Paul D. Moloney David A. Crook 《Ecology of Freshwater Fish》2015,24(3):443-455
Knowledge of the movement and habitat use of fishes is important in identifying and understanding the causes of population declines and predicting how populations are likely to respond to management interventions. In this study, radiotelemetry was used to examine the spring and summer movement and habitat use patterns of the freshwater catfish (Tandanus tandanus) in a remnant wetland to inform the development of recovery actions for this threatened species. Twenty‐one adult fish were tagged and released within Tahbilk Lagoon, Victoria, Australia, in September 2009. Fish were located every 1–2 weeks between September 2009 and February 2010, a period which coincides with the spawning period for the species. Eleven of the fish were also tracked every 2 h for 68 consecutive hours in December 2009 to examine diel movements. The study revealed that freshwater catfish make extensive use of cover (e.g. wood and macrophytes) and typically have limited ranges (median total linear range and 90% linear range 599 and 173 m respectively), although they occasionally moved more extensively (up to 1.5 km) between floodplain and riverine habitats. Fish moved over much greater areas at night compared with during the day. There was also evidence of sex‐specific variation in movement, with a trend for greater movement of female fish at night compared with males. The results of the study suggest that strategies that protect macrophyte and wood habitats and improve connectivity between riverine and floodplain habitats are likely to be important in maintaining and restoring remnant populations of this species. 相似文献
5.
Geoffrey G. Briggs George R. Cayley Glenn W. Dawson David C. Griffiths Ewen D. M. Macaulay John A. Pickett Mary M. Pile Lester J. Wadhams Christine M. Woodcock 《Pest management science》1986,17(4):441-448
Two analogues of the aphid alarm pheromone (E)-β-farnesene, a trifluorofarnesene and a difluoro-1-norfarnesene, were found to be highly active and were more readily detectable than the parent compound. For (Z)-hexadec-11-enal, a component of some lepidopteran sex attractant pheromones, replacement of the carbonyl oxygen with a difluoromethyl group to give a difluoroheptadecadiene resulted in loss of activity. A trifluoroacetoxyhexadecanolide was a more volatile analogue of the mosquito oviposition pheromone (?)-(5R,6S)-6-acetoxy-5-hexadecanolide and was highly active. 相似文献
6.
Suzanne J. Clark Nicola P. Coward Glenn W. Dawson Ian F. Henderson Andrew P. Martin 《Pest management science》1995,44(4):381-388
Two related iron chelates, one toxic to slugs by ingestion, the other not, were introduced into the foregut of D. reticulatum. The subsequent movement and redistribution of the metal within the slug tissues was studied by labelling the chelates with the radioactive isotope 55Fe. In slugs which survived treatment approximately half of the 55Fe was voided in faeces. The iron retained became unevenly distributed, the highest concentration occurring in the digestive gland, irrespective of the chelate used. At high doses, slugs treated with tris(1-oxo-1,2-diazabutan-2-oxido)Fe(III) were fatally poisoned while those treated with the homologue, tris(1-oxo-1,2-diazaoctan-2-oxido)Fe(III) were not. Slugs killed by the toxic chelate consistently contained proportionally less iron in the digestive gland and proportionally more in the body wall and reproductive system. Dosing slugs already killed by carbon dioxide asphyxiation gave a similar pattern, suggesting that the greater mobility of the iron from the toxic chelate was not a function of the slugs' metabolism. 相似文献
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Abstract— The treatment and control measures used during an outbreak of feline ringworm caused by Microsporum canis are described. During the outbreak the presence of the dermatophyte on the coats of clinically normal cats was demonstrated by the use of Mackenzie's brush technique. This method of examination is suggested as being the most effective and rapid means of ensuring that dermatophyte infection is not introduced into a colony by new cats. Résumé— L'auteur décrit le traitement et les mesures antiparasitaires appliqués à l'occasion d'une épizootie de teigne féline, provoquée par Microsporum canis. La préence du dermatophyte sur le poil de chats cliniquement normaux a été décelée par la technique de la brosse Mackenzie, méthode particulièrement rapide et efficace, propre à éviter la contamination d'une colonie de chats par des dermatophytes importés de l'extérieur. Zusammenfassung— Die Behandlung und die Kontrollmassnahmen während eines Ausbruchs von durch Microsporum caniJ verursachter Katzenringwurmkrankheit werden beschrieben. Wahrend der Krankheit wde das Vorhandensein der Dermatophyten in den Pelzen klinisch normaler Katzen durch Anwendung der Mackenzieschen Pinselmethode demonstriert. Diese Untersuchungs methode wird ah wirksamste und schnellste vorgeschlagen, die gewährleistet, dass die Dermatophyten nicht durch neue Katzen in eine Kolonie eingeschleppt werden. 相似文献
10.
Purpose The goal of our study was the evaluation of a practical method for the recording of flash electroretinograms (ERGs) in sedated, standing horses with the DTL? microfiber electrode. Methods The horses were sedated intravenously with detomidine hydrochloride (0.015 mg/kg). The pupil was dilated and the auriculopalpebral nerve was blocked. The ERGs were recorded with the active electrode on the cornea (DTL?), the reference electrode near the lateral canthus, and the ground electrode over the occipital bone. The light intensities of the white strobe light were 0.03 cd·s/m2 (scotopic) and 3 cd·s/m2 (scotopic and photopic). Photopic and scotopic single flash and flicker responses to Ganzfeld stimulation were recorded. During the 20‐min dark adaptation period the retina was stimulated every 5 min with the 0.03 cd·s/m2 single flash. Results The median b‐wave amplitudes and implicit times were 38 µV and 33 ms (photopic cone‐dominated response), 43 µV and 63 ms (5‐min dark adaptation), 72 µV and 89 ms (10 min), 147 µV and 103 ms (15 min), 188 µV and 109 ms (20 min, 0.03 cd·s/m2, rod response), and 186 µV and 77 ms (20 min, 3 cd·s/m2, maximal combined rod‐cone response). A steady increase in amplitude and implicit time was noted during dark adaptation. No oscillatory potentials could be isolated. Conclusions The use of detomidine hydrochloride sedation and the DTL? microfiber electrode allowed the recording of good quality ERGs. This protocol should permit the detection of functional problems in the retina without the risk involved with general anesthesia. 相似文献