Efficiency of SNP and SSR-based analysis of genetic diversity,population structure,and relationships among cowpea (<Emphasis Type="Italic">Vigna unguiculata</Emphasis> (L.) Walp.) germplasm from East Africa and IITA inbred lines

The extent of genetic diversity and relatedness of cowpea germplasm from East Africa are poorly understood. A set of 13 microsatellites (SSR) and 151 single nucleotide polymorphisms (SNPs) markers were applied to assess the levels of genetic diversity in a sample of 95 accessions of local cowpea germplasm and inbred lines of Vigna unguiculata. The average genetic diversity (D), as quantified by the expected heterozygosity, was higher for SSR loci (0.52) than for SNPs (0.34). The polymorphic information content was 0.48 for SSR and 0.28 for SNP while the fixation index was 0.095 for SSR and 0.15 for SNPs showing moderate differentiation and high gene flow among cowpea accessions from East African countries. The results of data analysis of both SSR and SNP markers showed similar clustering patterns suggesting a substantial degree of association between origin and genotype. Principal coordinate analysis (PCoA) with SSR and SNP markers showed that accessions were grouped into two and three broad groups across the first two axes, respectively. Our study found that SNP markers were more effective than SSR in determining the genetic relationship among East African local cowpea accessions and IITA inbred lines. Based on this analysis, five local cowpea accessions Tvu-13490, Tvu-6378, Tvu-13448, Tvu-16073, and 2305675 were identified to be tightly clustered sharing several common alleles with the drought tolerant variety Danila when analyzed with SSR and SNP markers. The findings will assist and contribute to future genetic diversity studies aimed at the genetic improvement of local Eastern Africa cowpea accessions for improved overall agronomic performance in general and breeding for drought tolerant in particular.     

Liveweight performance of crossbred and zebu cattle grazing on native or rhodes grass pastures

Summary Two experiments are described involving 33 crossbred (mainly Simmental and Jersey × Zebu) and 33 Zebu (Horro and Boran) bulls and steers. In experiment I, growth rates of crossbred and Zebu cattle rotationally grazed on either native pasture or Rhodes grass(Chloris gayana) were compared during a 140 day grazing period at a stocking rate of 2.4 animals/ha. In experiment II, growth rate of cattle rotationally grazed for 140 days on native pasture was compared with that of a similar group grazed on Rhodes grass either rotationally or continuously at a stocking rate of seven animals/ha.On native pasture the average daily gain by Zebu animals was similar to that of the crossbreds whilst on Rhodes grass liveweight gain of Zebu animals was lower than that of the crossbreds. Daily gains of cattle were significantly (P<0.01) greater on continuous compared with rotationally grazed Rhodes grass.

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Rendimiento En Peso De Bovinos Cruzados Y Cebu En Pradera Nativa Y Pasto Rhodes

Resumen Se describen dos experimentos; el primero con 33 animales cruzados (Simmental y Jersey × Cebú) y el segundo con 33 Cebú (Horro y Boran) toros y novillos. En el experimento I, se compararon las ratas de crecimiento del ganado cruzado y del Cebú en pastoreo rotacional en pradera nativa o pasto Rhodes (Chloris gayana), durante 140 días, con una carga animal de 2.4 animales por hectárea. En el experimento II, se comparó la rata de crecimiento de un grupo de ganado en pastoreo rotacional en pradera nativa durante 140 días, con aquella de un grupo similar en pasto Rhodes con pastoreo rotacional o continuo, con una carga animal de siete por hectárea. En pradera nativa, el promedio de ganacia diaria de los animales Cebú fue similar a aquel del grupo de animales cruzados, mientras que en pasto Rhodes la ganancia de peso de los animales Cebú fue más baja que aquella de los animales cruzados. Las ganancias diarias de peso de los animales fueron significativamente (P<0.01) mayores en pastoreo contínuo, comparado con el pastoreo rotacional en pasto Rhodes.

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Performances Ponderales De Zebus Et De Metis Zebus Entretenus Sur Des Paturages Naturels Et Das Paturages D'Herbe De Rhodes

Résumé Deux expériences utilisant 33 métis (principalement Simmental et Jersey x Zébu) et 33 zébus (Horro et Boran), taureaux et bouvillons sont décrites.Dans la première, les gains de poids des métis et des zébus entretenus à raison de 2,4 animal à l'hectate par rotation, soit sur des pâturages naturels ou sur de l'herbe de Rhodes ont été comparés durant 140 jours.Dans la seconde expérience, le taux d'accroissement pondéral du bétail entretenu, par rotations des pâturages, pendant 140 jours sur des pâturages naturels a été comparé avec celui d'un groupe identique d'animaux entretenus sur de l'herbe de Rhodes soit par rotation, soit de façon permanente à raison de 7 têtes à l'hectare sur les pâturages naturels; le gain journalier de poids par zébu a été semblable à celui des métis tandis que le gain de poids des zébus entretenus sur de l'herbe de Rhodes a été inférieur à celui des métis. Les gains journaliers de poids ont été significativement (P<0,01) plus élevés chez les animaux en pacage permanent comparés avec ceux obtenus par la rotation des pâturages d'herbe de Rhodes.

     

Analyses of genetic diversity and population structure of anchote (Coccinia abyssinica (Lam.) Cogn.) using newly developed EST-SSR markers

Anchote (Coccinia abyssinica (Lam.) Cogn.) is a perennial root crop belonging to Cucurbitaceae family. It is endemic to Ethiopia and distributed over wide range of agro-ecologies. For further improvement and efficient conservation of this crop, characterization of its genetic diversity and its pattern of distribution is a vitally important step. Expressed sequence tags-simple sequence repeats (EST-SSRs) markers were developed from publicly available watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] ESTs in the GenBank database. Among those novel markers, eight were polymorphic and subsequently used for genetic diversity and population structure analyses of 30 anchote accessions collected from western Ethiopia. A total of 24 alleles were obtained across the eight polymorphic loci and 30 accessions that revealed moderate level of genetic diversity in this minor crop. Among the eight loci, locus CA_06 was the most informative with six alleles and polymorphic information content (PIC) of 0.76. The accessions showed about threefold variation in terms of genetic diversity, with expected heterozygosity (He) ranging from 0.15 (accession An) to 0.44 (accession Dg). Other accessions with higher genetic diversity include Ar and Gu (He?=?0.43 and 0.41, respectively). Analysis of molecular variance (AMOVA) revealed that the variation within accessions and among accessions accounted for 84.7% and 15.3% of the total variation, respectively. The study revealed low but significant population differentiation in this crop with no clear pattern of population structure. The EST-SSR markers developed in this study are the first of their kind for anchote and can be used for characterization of its wider genetic resources for conservation and breeding purposes.

     

Furanosesquiterpenes from Commiphora sphaerocarpa and related adulterants of true myrrh

A new furanosesquiterpene, (1E)-8,12-epoxygermacra-1,7,10,11-tetraen-6-one (3), was isolated from the resin of Commiphora sphaerocarpa together with the known compounds curzerenone (1), furanodienone (2), (1E)-3-methoxy-8,12-epoxygermacra-1,7,10,11-tetraen-6-one (4), (1(10)E,2R*,4R*)-2-methoxy-8,12-epoxygermacra- 1(10),7,11-trien-6-one (5), and dihydropyrocurzerenone (6). Hydrodistillates of the resins of C. sphaerocarpa, C. holtziana, C. kataf and C. myrrha were analysed. The identifications were aided by NMR, GC and GC-MS.     

Genetic diversity of <Emphasis Type="Italic">Guizotia abyssinica</Emphasis> (L. f.) Cass. (Asteraceae) from Ethiopia as revealed by random amplified polymorphic DNA (RAPD)

Genetic diversity of 70 populations of niger (Guizotia abyssinica) representing all its growing regions in Ethiopia was investigated using random amplified polymorphic DNA (RAPD) to reveal the extent of its populations genetic diversity. Ninety-seven percent of the loci studied was revealed to be polymorphic for the whole data set. The within population diversity estimated by Shannon diversity index and Nei gene diversity estimates was revealed to be 0.395 and 0.158, respectively. The extent of genetic variation of populations from major niger producing regions was significantly lower than that of populations from other regions; however, it is distributed regardless of altitude of growth. Genetic differentiation between populations was estimated with Shannon index as G^′ _ST (0.432), Nei’s G _ST (0.242) and AMOVA based F _ST (0.350) and appears to be equivalent to the average values calculated from various RAPD based studies on outcrossing species. Higher proportion of the variation detected by AMOVA resided within populations (64.58%) relative to the amount of variation among populations (35.42%). UPGMA cluster analysis showed that most of the populations were clustered according to their region of origin. However, some populations were genetically distant from the majority and seem to have unique genetic properties. It is concluded that the crop has a wide genetic basis that may be used for the improvement of the species through conventional breeding and/or marker assisted selection. Collection of germplasm from areas not yet covered and/or underrepresented is the opportunity to broaden the genetic basis of genebank collection.     

Improving adaptation to drought stress in white pea bean (Phaseolus vulgaris L.): Genotypic effects on grain yield,yield components and pod harvest index

Common bean (Phaseolus vulgaris L.) is the most important food legume crop in Africa and Latin America where rainfall pattern is unpredictable. The objectives were to identify better yielding common bean lines with good canning quality under drought, and to identify traits that could be used as selection criteria for evaluating drought‐tolerant genotypes. In all, 35 advanced lines were developed through single seed descent and evaluated with a standard check under drought and irrigated conditions at two locations over 2 years in Ethiopia. Grain yield (GY), pod number per m², seed number per m² and seed weight decreased by 56%, 47%, 49% and 14%, respectively, under drought stress. Eight genotypes had better yield with good canning quality under drought compared to the check. Moderate to high proportion of genetic effects were observed under drought conditions for GY and yield components compared to genotype × environment effects. Significant positive correlations between GY and pod harvest index (PHI) in drought suggest that PHI could be used as an indirect selection criterion for common bean improvement.     

Protein quality and endosperm modification of quality protein maize (Zea mays L.) under two contrasting soil nitrogen environments

Quality protein maize (QPM) breeding involves the combined use of the opaque-2 (o2) gene and the genetic modifiers of the o2 locus to develop cultivars with modified kernel endosperm, and increased concentrations of lysine and tryptophan. This study was designed to assess grain yield performance, endosperm modification, and protein quality and quantity under two contrasting soil nitrogen environments. A 15-parent diallel cross was evaluated under one low nitrogen stress and one optimal nitrogen environment each at Harare (Zimbabwe) and Bako (Ethiopia). Most QPM hybrids showed higher protein quality levels than the best non-QPM check under both conditions. Protein concentration tended to vary across nitrogen levels, but not endosperm type. Significant differences were found for the test of main effect (nitrogen-level) for endosperm modification and tryptophan concentration. This indicated that QPM maintains quality even under low soil nitrogen, a widespread condition in Africa. General combining ability (GCA) mean squares were highly significant for most protein quality traits for each environment and across environments whereas specific combining ability (SCA) mean squares were not significant in most cases. This indicated that additive gene effects were primarily responsible for variation of most traits evaluated and hence progeny performance can adequately be predicted on the basis of parental performance. Inbred lines P2, P4 and P12 had desirable GCA effects for endosperm modification while P1 and P3 had the best GCA for tryptophan concentration in grain. The current study suggests that hybrids with desirable endosperm modification, protein quality and stable performance under low nitrogen stress and optimal conditions can be produced with careful selection.