Lymphocytic-plasmacytic enteritis in two horses

The primary hematologic abnormalities in 2 adult horses with chronic weight loss were hypoalbuminemia and hyperglobulinemia. One horse was anemic, had subclinical disseminated intravascular coagulation, and prolonged plasma sulfobromophthalein half-life. Small-intestinal dysfunction with malabsorption was indicated by abnormal D-xylose absorption test results. Clinicopathologic and pathologic findings were consistent with a diagnosis of malabsorption and protein-losing enteropathy, attributable to lymphocytic and plasmacytic infiltration of the intestine.     

Above- and belowground biomass measurements in an unthinned stand of Sitka spruce (<Emphasis Type="Italic">Picea sitchensis</Emphasis> (Bong) Carr.)

Reporting carbon (C) stocks in tree biomass (above- and belowground) to the United Nations Framework Convention on Climate Change (UNFCCC) should be transparent and verifiable. The development of nationally specific data is considered ‘good practice’ to assist in meeting these reporting requirements. From this study, biomass functions were developed for estimating above- and belowground C stock in a 19-year-old stand of Sitka spruce (Picea sitchensis (Bong) Carr.). Our estimates were then tested against current default values used for reporting in Ireland and literature equations. Ten trees were destructively sampled to develop aboveground and tree component biomass equations. The roots were excavated and a root:shoot (R) ratio developed to estimate belowground biomass. Application of the total aboveground biomass function yielded a C stock estimate for the stand of 74 tonnes C ha⁻¹, with an uncertainty of 7%. The R ratio was determined to be 0.23, with an uncertainty of 10%. The C stock estimate of the belowground biomass component was then calculated to be 17 tonnes C ha⁻¹, with an uncertainty of 12%. The equivalent C stock estimate from the biomass expansion factor (BEF) method, applying Ireland’s currently reported default values for BEF (inclusive of belowground biomass), wood density and C concentration and methods for estimating volume, was found to be 60 tonnes C ha⁻¹, with an uncertainty of 26%. We found that volume tables, currently used for determining merchantable timber volume in Irish forestry conditions, underestimated volume since they did not extend to the yield of the forest under investigation. Mean stock values for belowground biomass compared well with that generated using published models.     

Moderate sheep grazing in semiarid shrubland alters small-scale soil surface structure and patch properties

We investigated whether long-term moderate livestock grazing by sheep (ca. 10 animal unit days/ha) in a semiarid shrubland with a long-term average annual rainfall of 200 mm causes changes in soil surface structure and dimensions of shrub and intershrub patches. We examined grazing-induced changes in landscape patchiness, patch structure, and soil moisture in three grazed and three ungrazed plots of 4 m × 4 m on a south-facing slope and the opposite, more productive north-facing slope. The measurements were done in early spring 2001 and 2002 before grazing started, in two surveys, one using two parallel transects and one using sample quadrats of 20 cm × 30 cm under three shrubs per plot and on an adjacent part of the intershrub matrix. On the north-facing slope, the sheep reduced shrub patch size as they trampled the soil mounds under the shrubs and browsed the shrub canopy. Reduced shrub patch size decreases the area and resources available for plant production. On the south-facing slope, the sheep mainly disrupted the soil crust in the intershrub area. This may increase soil erosion, but also seedling establishment. The contrast in impacts on the two slopes is due to the interaction between environment (productivity, exposure and vegetation) and sheep behavior (herbivory and trampling). On both slopes, the changes due to grazing are significant, though small. Changes in patch size and properties induced by moderate grazing can have positive and negative effects on productivity and diversity, but may also be viewed as early signs of landscape degradation as is often caused by heavy grazing.     

Liquid chromatographic method for quantitation of glyphosate and metabolite residues in organic and mineral soils, stream sediments, and hardwood foliage

A liquid chromatographic method for determining glyphosate (GLYPH) and its major metabolite aminomethylphosphonic acid (AMPA) in various environmental substrates is described. Ion-exchange column chromatography is coupled with post-column ninhydrin derivatization and absorbance detection at 570 nm. Use of a valve-switching technique allowed quantitation of both analytes in a single chromatographic run and eliminated slow-eluting, coextracted interferences. The method was successfully used to quantitate GLYPH and AMPA in organic and mineral soils, stream sediments, and foliage of 2 hardwood brush species. Mean recovery efficiencies for GLYPH as determined from fortified blank field samples were as follows: bottom sediment 84%, suspended sediment 66%, organic soils 79%, mineral soils 73%, alder leaf litter 81%, salmonberry leaf litter 84%, and artificial deposit collectors 87%. Precision for GLYPH determination was good with less than 14% coefficient of variation on mean recovery for all substrates. Limits of detection were lowest for sediments (0.01 microgram/g dry mass) and highest for foliage substrates (0.10 microgram/g dry mass). Using this system, 6 samples/person/day were routinely analyzed.     

Potential use of rare earth oxides as tracers of organic matter in grassland

Tracing organic matter (OM) in soil is challenging, because runoff and leaching processes are interrelated and have multiple sources. Therefore, multiple tracers with low background concentrations such as rare earth element oxides (REOs) are necessary to delineate the origin of sources of the organic materials in groundwater, rivers or in catchments. The main objective of this study was to examine the potential use of REOs as a tracer in various forms of OM (1) whole slurry, (2) solid, and (3) liquid phase of cattle slurry after mechanical separation. A laboratory experiment was carried out using five REOs (La, Gd, Sm, Pr, and Nd oxides) mixed directly into soil or mixed with various fractions of cattle slurry and then applied to the soil surface. In the additional grassland experiment, Gd oxide was spiked with soil and cattle slurry and then applied to the soil surface. The mineral N in the liquid phase (urine) of the slurry in the grassland experiment was labelled with ¹⁵N urea (16 atom%). In the laboratory experiment, results showed that the five REOs concentration of soil in 0–1 cm soil section after the rainfall simulation was still up to 20 times more than the background values. In 1–2 cm soil section, the concentration of only Gd (two fold higher) and La oxides (50% higher) were significantly higher than the soil background values. Therefore, we hypothesized that Gd and La oxides were associated also with relatively finer organic particles in slurry, thus 1–2 cm soil section were enriched with these oxides. The five REOs concentration below 2 cm soil depth were similar to the background values in all treatments. In line with the laboratory experiment, Gd concentrations in the deeper soil layers (2–4 and 4–8 cm) in the grassland experiment were not significantly affected by any treatment. Both in grassland and laboratory experiment, solid phase of the slurry (dung) was collected from the soil surface after rainfall simulation. Here, about 56% of REOs were measured on the solid phase of the slurry which indicates the strong binding potential of REOs on slurry OM. The present novel study, where REO tagged slurry was uniquely tested to study geochemical cycle of organic fertilizers, clearly highlighted the potential for their use as multiple‐tracers of (animal derived‐) OM in agricultural soils.     

Sample collection and preparation in cytology: increasing diagnostic yield.

Cytology is a valuable diagnostic tool for examining many types of lesions. The diagnostic yield of cytology is dependent on optimal sample collection and smear preparation. This article outlines the basic techniques for collection of samples via fine-needle biopsy, imprints, scrapings, and swabs. Tips are given on how to avoid some of the most common problems that lead to nondiagnostic samples.     

Disseminated histoplasmosis in cats: 12 cases (1981-1986)

Anemia, weight loss, lethargy, fever, anorexia, and interstitial lung disease were the predominant clinical findings in 12 cats with disseminated histoplasmosis. Some cats were examined because of dysfunction or lesions of bone, eyes, or skin. In most cases, the clinical signs were observed by the owner for 4 weeks or less before seeking veterinary care. Young cats were most commonly affected, with 7 of the 12 cats less than or equal to 1 year old. Identification of Histoplasma organisms in bone marrow aspirates was used to confirm the diagnosis of histoplasmosis in 11 of the 12 cats. Histoplasma infection of multiple organs was found at necropsy. In this study, disseminated histoplasmosis had a higher prevalence in cats than in dogs at the same veterinary medical teaching hospital. Feline disseminated histoplasmosis was not associated with FeLV infection. Treatment was attempted in 7 of the 12 cats.