Einflu? von Jahrringbreite und Alter auf den mikroskopischen Bua von Trauben- und Stieleichenholz

Ohne ZusammenfassungÜberarbeiteter Teil einer Ingenieur-Abschlußarbeit an der Staatlichen Ingenieurschule für Holztechnik in Rosenheim des letztgenannten Verfassers.     

Studies on the excretion of estrogen in cows following oral and percutaneous (pour-on) administration of chlormadinone acetate]

The oestrogen levels were assessed in 180 urine samples taken from 20 cows. The animals had received over 15 days 40 mg one-per-cent oily solution of chloromadinone acetate. The solution had been applied orally or by pour-on methods. The curves of oestrogen excretion were identical for both methods of application, both during and after completed administration. In other words, the effect obtainable from pour-on application is just as good as that obtainable from oral administration.     

Determination of capsaicinoids in habanero peppers by chemometric analysis of UV spectral data

A novel spectrophotometric method for the determination of capsaicinoids in habanero pepper extracts is described that does not require prior analyte separation. The method uses partial-least-squares (PLS-1) multivariate regression modeling techniques in conjunction with ordinary UV absorption spectral data obtained on alcoholic extracts of habanero peppers (Capsicum chinese). The PLS-1 regression models were developed by correlating the known total concentration of the two major capsaicinoids (capsaicin and dihydrocapsaicin) in the extracts as determined by high-performance liquid chromatography with the spectral data. The regression models were subsequently validated with laboratory-prepared test sets. The validation studies revealed that the root-mean-square error of prediction varied from 4 to 8 ppm, based on the results obtained from models prepared from nine test sets. Once a regression model has been developed and validated, analyses of the extracts can be accomplished rapidly by ordinary spectrophotometric procedures without any prior separation steps.     

An evaluation of three paddlewheel aerators used for emergency aeration of channel catfish ponds

Oxygen transfer rate, power requirement and fuel consumption were determined for three paddlewheel aerators used for emergency aeration of channel catfish ponds. The power requirement of the tractor-powered units was directly related to the diameter of the paddlewheel drum and the paddle immersion depth. Oxygen transfer rates ranged from 6·9 to 41 kg h^?1 and increased linearly with the power requirement. The largest paddlewheel aerator, operated at the maximum paddle depth, produced the highest oxygen transfer rate (41 kg h^?1). Oxygen transfer efficiencies ranged from 1·29 to 1·97 kg kWh^?1.     

Stability of co‐composted hydrochar and biochar under field conditions in a temperate soil

Hydrothermally converted biomass (hydrochar) is evaluated as a carbon‐rich soil amendment in addition to pyrogenic biochar. After assessing the suitability of hydrochar for use in agriculture, its environmental safety and comparing its chemistry with that of biochar, we describe a field trial established at Halle (Germany) under natural conditions for a temperate climate and without further external management practices. The main objective of our study was to analyse the stability and hence the C sequestration potential of composted chars over a period of 2 years. Four treatments (no amendment control, compost, co‐composted hydrochar and co‐composted biochar) in fourfold field replication were chosen to make a direct comparison of biochar and hydrochar under field conditions. The total organic carbon and total N increased in all treatments in comparison with the control but only in biochar‐amended treatments were N concentrations more stable. Composted biochar showed significantly more black carbon content in topsoil, sampled some months after application, compared with all other treatments. We show that hydrochar is less suitable for long‐term C sequestration in comparison with biochar but has potential for soil amelioration because it delivers essential nutrients. On the other hand, biochar is richer in polyaromatic C than hydrochar and therefore is more stable in the long term. We assessed biochar stability using the black carbon analysis of the different soil samples.     

Distortion of genetically modified organism quantification in processed foods: influence of particle size compositions and heat-induced DNA degradation

Milling fractions from conventional and transgenic corn were prepared at laboratory scale and used to study the influence of sample composition and heat-induced DNA degradation on the relative quantification of genetically modified organisms (GMO) in food products. Particle size distributions of the obtained fractions (coarse grits, regular grits, meal, and flour) were characterized using a laser diffraction system. The application of two DNA isolation protocols revealed a strong correlation between the degree of comminution of the milling fractions and the DNA yield in the extracts. Mixtures of milling fractions from conventional and transgenic material (1%) were prepared and analyzed via real-time polymerase chain reaction. Accurate quantification of the adjusted GMO content was only possible in mixtures containing conventional and transgenic material in the form of analogous milling fractions, whereas mixtures of fractions exhibiting different particle size distributions delivered significantly over- and underestimated GMO contents depending on their compositions. The process of heat-induced nucleic acid degradation was followed by applying two established quantitative assays showing differences between the lengths of the recombinant and reference target sequences (A, deltal(A) = -25 bp; B, deltal(B) = +16 bp; values related to the amplicon length of the reference gene). Data obtained by the application of method A resulted in underestimated recoveries of GMO contents in the samples of heat-treated products, reflecting the favored degradation of the longer target sequence used for the detection of the transgene. In contrast, data yielded by the application of method B resulted in increasingly overestimated recoveries of GMO contents. The results show how commonly used food technological processes may lead to distortions in the results of quantitative GMO analyses.