Nutrient intake of horses in Thoroughbred and Standardbred stables

SUMMARY Twenty-five Thoroughbred (TB) and 25 Standardbred (SB) stables were visited to determine their feeding practices. The ingredients of the main feed of the day for a mature gelding of average size in full training were weighed at each stable. Nutrient content of diets was calculated using published data for the individual ingredients. Results are expressed as mean±sd. The estimated body weight of TB horses was 493±34 kg and 437±32 kg for SB horses. There was considerable variation in diet composition and nutrient intake between stables. The TB trainers fed 11.0±2.4 kg and SB trainers 11.8±2.5 kg per day. The concentrate component of the diet weighed 7.8±1.6 and 7.7±2.3 kg for TB and SB stables, respectively, and the roughage component for TB horses 3.3±1.4 and SB horses 4.1±1.4 kg per day. The digestible energy intake of horses at TB stables was 129±29 MJ per day and at SB stables 132±31 MJ per day. Crude protein intake of TB horses was 1452±363 g and SB horses 1442±338 g per day. There were differences in some feeding practices at TB and SB stables. Standardbred trainers fed more roughage than TB trainers. Standardbred trainers fed chaffed lucerne (alfalfa) and cereal hays as the major roughage, whereas TB trainers fed more hay. The major hay type fed by TB trainers was lucerne, whereas many SB trainers preferred clover hay. Both trainers fed oats as the major grain, but TB trainers fed slightly more maize (corn) than SB trainers. The SB trainers fed barley as part of the concentrate component of the diet, whereas TB trainers usually fed boiled barley and linseed oil in winter only. Although many trainers used vitamin and mineral supplements, this appeared unnecessary in many Instances, especially with respect to Iron. Calcium and NaCI supplementation was necessary for some diets. We concluded that while there was a wide range in feed intake and diet composition for both TB and SB horses, average nutrient intakes were similar to National Research Council (1989) recommendations for horses performing intense work.     

A nutritional myopathy enzootic in a group of yearling beef cattle

Steers and bulls under feedlot conditions and on an apparently adequate ration developed transitory signs of diarrhea and unthriftiness. One animal became recumbent. Sick and clinically normal animals in the group had elevated serum creatine kinase and aspartate aminotransferase enzyme values and degenerative changes in muscle fibers on biopsy. Feed analysis was carried out and the animals were monitored over several weeks by means of muscle biopsy and serum enzyme analysis as well as by postmortem examination at slaughter. Subclinical myopathy was found in several animals and was thought to be related to low vitamin E content in the high moisture corn ration.     

The growth and nitrogen economy of rice under sprinkler and flood irrigation in South East Australia

Summary ¹⁵N balances were compared in rice (Oryza sativa L., cv. Calrose) grown under continuous flood (CF) or sprinkler irrigation. Two sprinkler treatments with irrigation frequencies of once (S1W) and thrice (S3W) per week were studied. Five atom %¹⁵N-labelled urea (60 kg N ha^–1) was applied to microplots either 36 or 84 days after emergence (DAE). An equivalent amount of unlabelled urea was applied at the other application time, so that each microplot received a total of 120 kg N ha^–1 in an equal split. There was no significant effect of irrigation treatment on recovery of urea N by straw. Straw recovery from urea applied 36 DAE was almost half that from an application 84 DAE, and time of urea application produced a similar effect on recovery in grain. Grain recovery in S1W was less than half that in CF and S3W for both application times. Total plant recovery of urea N applied 36 DAE was similar for all irrigation treatments (average 29%), but for urea applied 84 DAE total plant recovery in CF (67%) was significantly higher than in S1W (49%). Total N uptake in the plant tops was considerably lower in both the sprinkler-irrigated treatments when compared with CF, and this was mostly due to reduced soil N uptake in S3W (one-half) and S1W (one-third). The proportion of N derived from fertilizer in the plant tops increased from 40% in CF to 60% in S1W. Immobilization of applied N in the soil of the sprinkler-irrigated treatments was greater than in CF by factors of 1.5 (S3W) and 2 (S1W). Immobilization of urea N applied 36 DAE was almost 50% greater than immobilization of urea N applied 84 DAE. There was a trend for lower losses of fertilizer N with sprinkler irrigation (mean loss 18% of the applied N) compared with CF (27%). Within all irrigation treatments, the loss from urea applied 36 DAE was more than double the loss from urea applied 84 DAE. An additional study in CF compared the ¹⁵N balance for split application versus a single dose applied 36 DAE (before permanent flood). Split application resulted in significantly increased plant recovery of applied ¹⁵N, and this was largely associated with increased recovery in the grain. Slightly more fertilizer N was immobilized in the soil with a single application. The effect of application method on N loss was not significant.     

The growth and nitrogen economy of rice under sprinkler and flood irrigation in South East Australia

Summary Dry-seeded rice (Oryza sativa L., cv. Calrose) was subjected to 4 irrigation treatments — continuous flood (CF) and sprinkler irrigation at frequencies of one (S1 W), two (S2W) and three (S3W) applications per week — commencing 37 d after 50% emergence (DAE). The amount of water applied was calculated to replace water lost by pan evaporation. Urea (120 kg N ha^–1) was applied in a 1:1 split 36 and 84 DAE, and there were also unfertilized controls for each irrigation treatment. Amounts of nitrate (NO ₃ ^– ) in the soil were very low throughout the growing season in all treatments, despite regular periods of draining which lasted for up to 7 d in SlW. In all irrigation treatments, the majority of the fertilizer nitrogen (N) was located in the top 20 mm of soil. After each application of fertilizer, levels of mineral N in CF declined rapidly, while levels in S3W and S1W remained high for 1–2 weeks longer. The poor growth of sprinkler-irrigated rice was not due to lower amounts of mineral N in the soil. The greater persistence of fertilizer N in the sprinkler-irrigated treatments was probably due to reduced root activity near the soil surface because of frequent periods of soil drying in between irrigations. Net mineralization of soil N in the unfertilized sprinkler-irrigated treatments was reduced by about half compared with CF.On average, the quantity of water applied (1.2–1.4 × EP) to the sprinkler-irrigated treatments appeared to be sufficient to meet the evapotranspiration demands of the crop, except possibly around flowering time. However, the plants may have suffered from moisture stress in between irrigations. Soil matric potential data at 100 mm suggested little water stress in the sprinkler-irrigated treatments during the vegetative stage, consistent with the similar tiller and panicle densities in all irrigation treatments. However, the crop was stunted and yellow and leaf rolling was observed in the sprinkler-irrigated treatments during this period. Soil matric potential data at 100 mm indicated considerable water stress in S1W beyond the commencement of anthesis, and in S2W during grain filling, consistent with the reduced floret fertility and grain weight in those treatments.     

Genotype X environment interaction in Hereford cattle: IV. Postweaning traits of bulls

Genotype X environment interactions for postweaning performance traits of bulls produced by different lines of Hereford cattle were investigated in the contrasting environments of Miles City, Montana and Brooksville, Florida. During Phase 1 of the study (1966 to 1973), the performance of bull progeny from two unrelated lines (M1 and F6 previously developed in Montana and Florida, respectively) was compared at each of the two experimental sites. During Phase 2 (1967 to 1974), performance of bulls from two related lines (M1 of Montana origin and F4 derived from an M1 foundation through selection in Florida) was compared at each of the two locations. The line X location interaction effect in Phase 1 was highly significant for weaning weights and average daily gain during the postweaning test, and for end-of-test weight, conformation score, condition score and forecannon circumference. The interaction effect in Phase 2 was highly significant for 205-d and end-of-test weights and significant for all other traits except end-of-test conformation score. These results, consistent with results for traits covered in other papers of the same series (reproductive traits, birth-to-weaning traits of bull and heifer calves combined, and postweaning traits of heifers), indicated the existence of economically important genotype X environment interactions in beef cattle. Results from the series of papers indicated that adaptation to local environment should receive consideration in planning breeding programs, performance tests and interregional transfers of beef cattle.     

Relationship of Progesterone,Bovine Pregnancy-Associated Glycoprotein-1 and Nitric Oxide with Late Embryonic and Early Fetal Mortalities in Dairy Cows

The aim of the present study was to determine the relationship of progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 to day 42) and early fetal mortalities (EFM; > day 42 to day 56) in dairy cows. Transrectal ultrasonography (6–8 MHz) was performed in 100 Holstein-Friesian cows at days 28, 42 and 56 after artificial insemination (AI; day 0) to diagnose pregnancy and to monitor the fate of the embryo. After ultrasound scanning of each cow, a milk sample was collected for assessment of P4 by an ELISA test and a blood sample was collected for assessment of bPAG-1, by using a double-antibody radioimmunoassay, and serum NO metabolites (nitrate + nitrite). Based on ultrasonographic examinations and bPAG-1-RIA, 41 of 100 inseminated cows were confirmed pregnant at day 28 after AI. Nine cows suffered of LEM, and 6 cows suffered of EFM and the overall pregnancy loss rate was 36.6% (15/41) between days 28 and 56 of pregnancy. By logistic regression analysis, there were no significant relationships between the level of P4 and bPAG-1 at day 28 after AI and the occurrence of LEM and EFM. Also, there were no significant relationships between the levels of P4 and bPAG-1 at day 42 and the occurrence of EFM. On the other hand, a significant relationship (P<0.05) was found between NO level at day 28 and the occurrence of LEM. In conclusion, measurement of the serum NO concentration at day 28 of pregnancy might help to predict the outcome of pregnancy by day 42 in dairy cows but further studies are needed to confirm this.     

The Post‐Cervical Insemination does not Impair the Reproductive Performance of Primiparous Sows

The study evaluated the reproductive performance of primiparous sows submitted to post‐cervical insemination (PCAI) compared with cervical artificial insemination (CAI). Difficulty with catheter introduction, the occurrence of bleeding or semen backflow during insemination, and volume and sperm cell backflow up to 60 min after insemination were also evaluated. Sows were homogenously distributed, according to body weight loss in lactation, lactation length, weaned piglets, weaning‐to‐oestrus interval and total born in previous farrowing, in two treatments: PCAI (n = 165) with 1.5 × 10⁹ sperm cells in 45 ml (2.4 ± 0.04 doses per sow) and CAI (n = 165) with 3 × 10⁹ sperm cells in 90 ml (2.5 ± 0.04 doses per sow). During PCAI, sows were inseminated in the absence of boars. Transabdominal real‐time ultrasonography was performed at oestrus onset, immediately before the first insemination and at 24 h after last insemination. There was no difference (P > 0.05) between treatments in farrowing rate (91.5% × 89.1%) and litter size (12.5 × 11.9 piglets born, respectively for PCAI and CAI sows). Successful passage of the intrauterine catheter in all the inseminations was possible in 86.8% (165/190) of sows initially allocated to PCAI treatment. Difficulty of introducing the catheter in at least one insemination did not affect the reproductive performance of PCAI sows (P > 0.05). Bleeding during insemination did not affect (P > 0.05) the farrowing rate in both treatments, but litter size was reduced in CAI and PCAI sows (P ≤ 0.06). Percentage of spermatozoa present in backflow within 1 h after insemination was greater in CAI than PCAI sows (P < 0.01). More than 85% of primiparous sows can be successfully post‐cervical inseminated with doses containing 1.5 × 10⁹ sperm cells in the absence of the boar during insemination without impairing the reproductive performance.     

Field trial of a staphylococcal mastitis vaccine in dairy herds: clinical, subclinical and microbiological assessments

Objective To assess the efficacy of a new staphylococcal mastitis vaccine under commercial dairying conditions.
Design A field trial involving 1819 cows and heifers conducted on seven dairy herds in Victoria. The trial was done 'blind'; approximately half the animals were vaccinated and the remainder were untreated controls.
Procedure The vaccine was given twice during the last 10 weeks of pregnancy. Effects of vaccination were assessed, during the ensuing lactation, on the basis of clinical and sub-clinical mastitis and microbiological investigations of the milk.
Results A total of 273 cases of clinical mastitis were recorded. Staphylococcus aureus was isolated from 112 of these, 45 cases in vaccinates and 67 cases in controls; the difference was not statistically significant. One herd was notable in having a high incidence of clinical staphylococcal mastitis. This herd accounted for 15.8% of the animals in the field trial but 54.5% of cases of clinical staphylococcal mastitis. For this herd, vaccinated animals had significantly lower incidence of clinical staphylococcal mastitis and prevalence of subclinical mastitis, relative to controls. An unexpected feature of the trial as a whole was the low incidence of clinical mastitis from which S aureus was isolated in pure culture (26.3% of cases) and the high incidence of clinical Streptococcus uberis mastitis (22.7% of cases).
Conclusions The trial showed that the vaccine was efficacious in reducing the incidence of clinical mastitis and prevalence of subclinical mastitis in a herd that had a serious staphylococcal mastitis problem.