Effect of excess zinc and arbuscular mycorrhizal fungus on bioproduction and trace element nutrition of Tomato (Solanum lycopersicum L. cv. Micro-Tom)

ABSTRACT

The effect of excess Zn and arbuscular mycorrhizal (AM) fungus on bioproduction and trace element nutrition were investigated in tomato. In a completely randomized factorial design, the experimental treatments – Zn addition at 0 (normal) and 300 (excess) mg Zn kg^?1 soil, and AM inoculation (non-AM and Rhizophagus irregularis) – were set up in a growth chamber for 10 weeks. Generally, AM effects on the available Zn, Mn, Cu, and Fe in the rhizosphere soil were in tandem with the effects in host tissues. Under normal Zn condition, AM enhanced Cu availability in the rhizosphere, optimized the Cu:Zn balance in shoots, and increased the host biomass production. Excess Zn reduced mycorrhizal colonization in AM plants and the total plant biomass in both AM and non-AM plants. Although AM decreased the Zn concentrations in soil and host tissues under excess Zn, the distortions in host TE balance were not significantly ameliorated by the fungus. While Zn in fruit was within the safety threshold, Mn deficiency in the fruit was observed under excess Zn, alongside increased root-to-fruit Fe and Cu translocations. Mycorrhizal reductions in soil and tissue Mn concentrations were considered a minus in terms of probable symbiont amelioration of Mn:Zn in-balance under excess Zn. Additional microbe(s) that can enhance Mn homeostasis might be helpful in tomato under elevated soil Zn.     

Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice

Journal of Plant Diseases and Protection - The high cost of chemical pesticides and their negative impact on the environment prompted the search for natural pesticides from plants. The objective of...     

Species composition of grasshoppers (Orthoptera) in open plots and farmlands in calabar metropolis, southern Nigeria

The grasshoppers are strategic in the welfare of man and may constitute a major threat when its population is not checked. A study on the distribution of grasshoppers in open plots and farmlands was carried out within Calabar Metropolis between August to November, 2010. A total of 295 grasshoppers belonging to 11 species grouped under 3 families (Tettigoniidae, Acrididae and Pyrgomorphidae) were collected from 8 study locations. Grasshoppers were collected weekly from all study sites using sweep nets between 11 a.m. to 4 p.m. The collection was done using sweep nets between 11 a.m. to 4 p.m. when grasshoppers baked themselves under the sun. The percentage abundance of these species were Spathosterrium pygmaeum (16.27%), Tettigonia viridissima (11.86%), Catantops spissus (11.19%) Acridaturita sp. (10.17%), Gastrimargus acrididae (9.83%), Schistocerca nitens (9.49%), Tylopsis sp. (7.46%), Zonocerus variegatus (6.78%), Omocestus viridulus (6.10%), Scudderia mexicana (5.76%) and Zonocerus elegans (5.08%). Tettigonia viridissima and Acridaturita sp. were largely distributed as it occurred in 7 of 8 study sites while Scudderia mexicana was the least distributed, as it was reported in 3 sites only. The dominant grasshopper species in open plot was Spathosterrium pygmaeum (19%) in relative abundance and the least was Zonocerus variegatus (0.64%). Zonocerus variegatus was the dominant species in farmland (14%) in relative abundance and the least was Schistocerca nitens (4%). Chi-square test showed a high significant difference between the distribution of grasshoppers in open plots and farmlands (p < 0.05). Variations in grasshopper species composition were attributed to lizard predation and management practices such as grass cutting, fertilizer and pesticide applications. It was therefore concluded that species abundance and population of grasshoppers could be enhanced by minimizing human activities that interfere with land use.     

Bioactive Chromodorolide Diterpenes from an Aplysillid Sponge

The known chromodorolides A (1) and B (2), and a new derivative chromodorolide C (3) have been isolated from an Australian sponge and characterized by 1D and 2D NMR experiments. The chromodorolides exhibited significant cytotoxicity against the P388 mouse leukaemia cell line, and also showed activity against the free-living larval stages of the parasitic nematodes Haemonchus contortus and Trichostrongylus colubriformis.     

Evaluation of environmental impact of the raised-bed-dike (Rong Chin) system along the Tha Chin river in Suphan Buri-Nakhon Pathom Provinces,Thailand

Vegetable cultivation on an embankment surrounded by water (raised-bed-dike system, Thai name rong chin) along the Tha Chin (Suphan Buri) river in the Suphan Buri and Nakhon Pathom Provinces was surveyed. The system was introduced by Chinese immigrants to produce vegetables in the river delta. The rong chin system in this area produced mainly Chinese kale, chili, bitter gourd, yard-long bean, taro, and sweet potato, while fruit trees and flowers were seldom cultivated, because of the flooding of the river in the rainy season. The amounts of nitrogen and phosphorus applied into field soils as fertilizers were far higher than those recovered in the harvested vegetables, indicating that only a limited portion of the nutrients was taken up by the crops and that part of the nutrients remained in the soils and in the dike water. Periodical flooding promoted leaching of the accumulated nutrients in the fields. Analyses of the soils and river waters revealed that phosphorus had started to accumulate in the environment, whereas only a small amount of nitrogen was detected in the river, canal, and dike waters, presumably because of the active uptake of nitrogen by biomass and denitrification.     

Soil organic carbon,nitrogen, and phosphorus distribution in stable aggregates of an Ultisol under contrasting land use and management history

Different land‐use affects the organization of mineral soil particles and soil organic components into aggregates and the consequent arrangement of the aggregates will influence essential ecosystem functions. We investigated a continuous rubber plantation (forested), land fallowed for 10 y (fallow), 10‐y continuous arable cropping land and cropped land with top soil removed (TSR) for concentrations of C, N, and P in bulk soil and dry aggregates. Results showed that a high level of soil disturbance decreased the proportion of surface (0–15 cm) soil aggregate stability (low mean weight diameter) in TSR by 149% and arable cropping by 125% compared with the forested. Aggregate associated SOC was higher in aggregate‐size fractions of forested land‐use when compared with that in 10‐y fallow, continuous arable cropping, and TSR. For aggregate associated N, fallow and forested land‐use types concentrated higher proportion across aggregate sizes than the arable cropping and TSR. Macro aggregate fractions generally contained higher concentrations of C, N, and P compared with the micro‐aggregates. Water transmission indicators like total porosity and saturated hydraulic conductivity recorded higher values with forested and fallow land‐use than the others. We can thus conclude that long‐term soil disturbance due to cultivation and removal of top soil reduces the accumulation of soil C, N, and P in bulk soil and decreases water transmission properties. On the other hand, aggregate‐associated C, N and P accumulations are dependent on the level of soil surface disturbance and aggregate sizes.     

Effect of guava Psidium guajava (L.) aqueous extract diet on growth performance,intestinal morphology,immune response and survival of Oreochromis niloticus challenged with Aeromonas hydrophila

An 84‐day feeding trial was carried out to evaluate the effects of aqueous Psidium guajava leaf extract (PGE) on growth, intestinal morphology, physiology, immune response and susceptibility of Oreochromis niloticus to Aeromonas hydrophila. Diets containing 0% (P0), 0.25% (P1), 0.50% (P2), 0.75% (P3) and 1.00% (P4) PGE were fed to triplicate groups of fish (mean weight; 1.32 ± 0.04 g) for 84 days. After the 84‐day feeding trial, test fish were injected with pathogenic A. hydrophila and then fed for 14 days. More feed were consumed in groups of fish fed PGE‐treated diets and resulted in significantly higher weight gain and feed intake. Incidentally, there was an increase in the calculated area of absorption of fish fed PGE diets, as accounted for by marginally higher villi length and width. Antioxidant and immune response were improved with PGE inclusion in diets as total protein, superoxide dismutase, glutathione peroxidase and glutathione S‐transferase significantly increased (p < 0.05) in fish fed PGE diets. Results of the challenge test with A. hydrophila revealed that the highest mortality (100%) was recorded in P0. This study revealed that inclusion of P. guajava extract in the diet of O. niloticus improved growth, nutrient utilization, immune system and survival of O. niloticus fingerlings.     

Soil penetrating quality in cultivated and forested coastal plain sands of Southern Nigeria

Conversion of natural forest to intensive cultivation makes to soil susceptible to flooding, declining fertility and loss of organic matter (OM) and reduced water movement into and within the soil. We studied infiltration rates and related soil penetrating indicators of forested and cultivated soils in humid tropical coastal plain sands in Southern Nigeria. Results showed that mean-weight diameter (MWD) and water stability of aggregates were higher in forested than cultivated soils. Stable aggregates > 1.00 mm were 16.5% and 31.1% respectively, for cultivated and forested soils at 0–15 cm depth, indicating formation of more macro-aggregates in forested soil. Soil disturbance through cultivation decreased hydraulic conductivity and increased bulk density of the soil. Infiltration rate attained after 2 hours was higher in forested soil. Temporary infiltration rate of 178 mm hr^?1 at initial time in cultivated soil was followed by very low infiltration rate of 7 mm hr^?1 after 2 hours. Soil organic matter (SOM), saturated hydraulic conductivity, MWD and total sand correlated positively with infiltration rates are r = 0.76, 0.61, 0.57 and 0.51 respectively. Changes in these parameters are dependent on surface soil disturbance by cultivation. Cultivation of forest decreased infiltration rates and water transmission properties of the soil.     

Diplazium sammatii: Athyraceae (`Nyama Idim'): Age-related nutritional and antinutritional analysis

Tender and mature leaflets and leaves of Diplazium sammatii (`Nyama idim'), were separately investigated for their nutritional and antinutritional properties. Results showed little difference in proximate nutritional contents between tender and mature leaves, except for crude fat, which was present in levels 1.5 times higher in younger than in older leaves. Crude fiber levels were low. Zinc (Zn) and copper (Cu) were higher in younger leaves (6.8 and 3.5 mg/100 g dry matter, respectively, compared to 4.5 and 2.5 mg/100 g dry matter, respectively in older leaves). Older leaves contained higher levels of iron (Fe): 6.7 mg/100 g dry matter, in comparison to 4.3mg/100 g dry matter, in young leaves. Oxalate levels were slightly higher in younger than older leaves. However, the reverse was true for tannin content. Both antinutritional compounds were present in levels which shouldbe safe.