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1.
Boy J.H.M. Possen Elina OksanenMatti Rousi Hanna RuhanenViivi Ahonen Arja TervahautaJaakko Heinonen Juha HeiskanenSirpa Kärenlampi Elina Vapaavuori 《Forest Ecology and Management》2011,262(8):1387-1399
For northern Europe’s climate, models predict an increase in periods of drought and waterlogging. Knowledge of variation between genotypes of Betula pendula (birch) and Populus tremula (aspen) to drought and excess soil moisture are unavailable but relevant for future development of forest ecosystems. We studied variation between genotypes to soil moisture in birch and aspen with plant material representing naturally regenerated populations and showed differences in acclimation to soil moisture conditions. Genotypes showing most growth and biomass accumulation across treatments maintained most leaf area, high gas exchange and water-use efficiency and grew most root mass but had the lowest root length per unit root dry mass compared to other genotypes. This indicates that these genotypes are more efficient in harvesting water from the soil under adverse conditions. We also showed that birch and aspen employ different strategies to cope with soil moisture conditions, with aspen investing more in perennial parts, while birch efficiently maintains foliar processes. When the expression of some known drought responsive genes was measured, only ACC oxidase was induced by the drought treatment at the beginning of the experiment, while surprisingly LEA5, RD22 and ADH1 did not respond to drought, but were up-regulated in prolonged wet conditions indicating oxidative stress and hypoxia and that these genes are responding to multiple stress factors. We conclude that in plants micro-propagated from naturally regenerated birch and aspen populations, there is variation between genotypes in acclimation efficiency to soil moisture conditions. 相似文献
2.
Kautto AH Alenius S Mossing T Becher P Belák S Larska M 《Veterinary microbiology》2012,156(1-2):64-71
Herding semi-domesticated reindeer has economic and social value for Sami people in the northern territories of Fennoscandia. However, with the intensification of reindeer husbandry, interspecies transmission of pathogens between reindeer and domestic animals may become a problem, especially for countries such as Sweden, Norway, and Finland where pestivirus and alphaherpesvirus have been eradicated in domestic ruminants. This study, which included 1158 Swedish reindeer, showed relatively high prevalence of antibodies against bovine viral diarrhoea virus (BVDV) (32%) and bovine herpesvirus-1 (BoHV-1) (53%). Adult animals were more often seropositive for BVDV and BoHV-1 (50% and 78%, respectively) than were calves (18 and 11%, respectively). While the seroprevalence of alphaherpesvirus was similar in different herding districts, pestivirus seropositivity was highest in the South and diminished towards the North of the Swedish reindeer herding area. High correlation of the seropositivity against both pathogens at both individual and herd levels may indicate possible mutual synergetic effects and may be explained by the immunosuppressive nature of the viruses. While alphaherpesvirus seroprevalence was probably related to putative cervid herpesvirus 2 (CvHV-2), the pestivirus infecting reindeer remains undefined. The virus neutralisation test of reindeer sera using different pestivirus strains, revealed higher titres against Border disease virus strains like 137/4 (BDV-1) and Reindeer-1 (BDV-2) than against BVDV-1. However, the virus was not identified by real time RT-PCR in any of the samples (n=276) from seronegative reindeers. The study showed that pestivirus and alphaherpesvirus infections are endemic in the Swedish reindeer population. 相似文献
3.
Wilhelmson A Laitila A Vilpola A Olkku J Kotaviita E Fagerstedt K Home S 《Journal of agricultural and food chemistry》2006,54(2):409-416
The steep water is generally aerated in industrial barley malting. However, it is questionable whether oxygen actually reaches the embryo, which remains entrapped under the husk, testa, and pericarp until chitting occurs. The aim of our study was to investigate whether barley embryos experience oxygen deficiency during steeping, and whether various steeping conditions affect the oxygen deficiency. Alcohol dehydrogenase Adh2 was induced in all steeping conditions studied. Therefore, oxygen deficiency occurred regardless of the steeping conditions. However, steeping conditions affected the rate of recovery from oxygen deficiency, germination rate, and onset of alpha-amylase production. When barley was subjected to oxygen deficiency by applying N(2) gas during steeping, the timing of the treatment determined its effects. The importance of aeration increased as the process proceeded. Oxygen deprivation at the beginning of the process had little effect on malt quality. Therefore, the timing of aeration is important in the optimization of germination during the steeping stage of malting. 相似文献
4.
Pirjo S.A. Mäkelä Antti Tuulos Marja Turakainen Arja Santanen Frederick L. Stoddard 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):195-201
Abstract Winter turnip rape (Brassica rapa spp. oleifera) is an underutilized crop that deserves to be revitalized for use in high-latitude agriculture. Many crop rotations around the world are dependent on the small-grain cereals, and turnip rape as a break crop, with its range of secondary chemicals, helps to suppress weeds, nematodes and pathogenic fungi. It may be used as an energy crop, it can restrict erosion and nutrient leaching while also improving soil structure and fertility, and it requires relatively low inputs. Although winter turnip rape was once the major oil crop in Finland, in the 1970s it was replaced by spring turnip rape, the lower erucic acid and glucosinolate contents of which made it suitable for food and feed uses. Winter hardiness of the crop could be improved, and industrial end uses, such as lubricants for which high erucic acid content is preferred, targeted in the first instance. Breeding progress would be accelerated by a change from the predominantly self-incompatible breeding system to self-compatibility, now available in modern germplasm, and this would allow use of other rapid breeding methods, such as doubled haploidy. Thus, the many advantages of the winter turnip rape crop would repay its return to agriculture. In this review we will introduce the many utilization possibilities of the crop as well as give background on why more attention and research efforts should be paid towards this crop. We will also indicate some of the array of factors that have a marked role in an attempt to ecologically intensify crop production. 相似文献
5.
Suppression subtractive hybridization (SSH) was used to isolate genes differentially expressed following exposure to copper (Cu) in a naturally selected Cu-tolerant birch (Betula pendula Roth.) clone originating from a disused lead/zinc smelter. Of the 352 cDNA fragments initially isolated, 108 were up-regulated by Cu, of which 55 showed over twofold induction by macroarray analysis. Searches against protein databases (Blastx) and sequence analysis provided the tentative identity of 21 genes. Three fragments lacked homology to any sequences in the databases. Most of the identified genes are involved in cellular transport, regulation or cell rescue and defense. Several genes have not previously been reported to be up-regulated by Cu, e.g., plasma intrinsic protein 2, glutamine synthetase and multi-drug resistance-associated protein (MRP4). The expression of MRP4, a vacuolar sorting receptor-like protein and an unidentified gene was studied in more detail by quantitative real-time PCR. These genes showed stronger up-regulation by Cu in the roots and shoots of the Cu-tolerant birch clone compared with a less tolerant clone. Clear clonal differences in gene expression were observed, e.g., for the regulator of chromosome condensation family protein, DnaJ protein homolog, vacuolar sorting receptor-like protein and MRP4. These findings contribute to our understanding of Cu tolerance in birch, a pioneer plant in metal-contaminated soils. 相似文献
6.
A. Wilhelmson K.‐M. Oksman‐Caldentey A. Laitila T. Suortti A. Kaukovirta‐Norja K. Poutanen 《Cereal Chemistry》2001,78(6):715-720
Germination can be used to improve the texture and flavor of cereals. However, germination generally causes breakdown of β‐glucans, which is undesirable with respect to the functional properties of β‐glucan. Our aim was to assess possibilities of germinating oat without substantial loss of high molecular weight β‐glucan. Two cultivars, hulled Veli and hull‐less (naked) Lisbeth were germinated at 5, 15, and 25°C and dried by lyophilization or oven drying. Elevated germination temperatures led to an increase in Fusarium, aerobic heterotrophic bacteria, Pseudomonas spp., lactic acid bacteria, enterobacteria, and aerobic spore‐forming bacteria. Therefore, the germination temperature should be kept low to avoid excessive growth of microbes. Of the samples germinated at 15°C, only one contained low amounts of the Fusarium toxin deoxynivalenol (52 μg/kg). Germination led to the breakdown of β‐glucans, but the decrease in the molecular weight of β‐glucan was initially very slow. A short germination schedule (72 hr, 15°C) terminated with oven drying was developed to produce germinated oat with retained β‐glucan content. Compared with the native oat, 55–60% of the β‐glucan could be retained. 相似文献
7.
Kati Katina Riikka Juvonen Arja Laitila Laura Flander Emilia Nordlund Susanna Kariluoto Vieno Piironen Kaisa Poutanen 《Cereal Chemistry》2012,89(2):126-134
The aim of the current study was to identify factors influencing the technological functionality of fermented bran. The influences of fermentation type and type of wheat bran on the microbial community, bioactivity, arabinoxylans (AX), and activity of xylanases were studied in the bran ferments. Furthermore, technological quality of ferments was established by using them to replace wheat in baking with a 20% substitution level. Solubilization of AX and endogenous xylanase activity of bran were influenced by the type of bran, fermentation type, and conditions. Peeled bran had a clearly reduced microbial load and different microbial community in comparison to native bran. Bran from peeled kernels contained 10‐fold lower activities of endogenous xylanases in comparison to native bran. Yeast fermentation of bran from peeled kernels increased the level of folates (+40%), free phenolic acids (+500%), and soluble AX (+60%). Bread containing yeast‐fermented peeled bran had improved volume (+10–15%) and crumb softness (25–35% softer) in comparison to unfermented counterparts. Solubilization of AX during the 20 hr fermentation and decreased endogenous xylanase activity are proposed as the main reasons for the improved technological functionality of fermented bran. 相似文献
8.
Arja Lilja Reijo Karjalainen Päivi Parikka Kari Kammiovirta Heikki Nuorteva 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):529-535
Phytophthora cactorum strains isolated from necrotic stem lesions on Betula pendula seedlings or from Fragaria
ananassa plants suffering from crown rot were pathogenic to their host plants. Only isolates from birch caused clear lesions on non-wounded bark of birch. P. cactorum isolates from birch were not detrimental to strawberry. Random Amplified Polymorphic DNA (RAPD) analysis revealed variation within P. cactorum, isolates from silver birch having different banding patterns than those from strawberry. UPGMA analysis clustered isolates from silver birch and strawberry plants into separate groups. The data show that the recent outbreak in Finland of P. cactorum in birch could not be caused by the import of strawberry plants affected by crown rot. 相似文献
9.
Anja I. Pekkarinen Tuija H. Sarlin Arja T. Laitila Auli I. Haikara Berne L. Jones 《Journal of Cereal Science》2003,37(3):349-356
Barley (Hordeum vulgare L.) that is infested with Fusarium head blight (FHB, ‘scab’) is unsuitable for malting and brewing because it may contain mycotoxins and has unacceptable malting quality. Fungal proteinases are apparently often involved in plant-microbe interactions, where they degrade storage proteins, but very little is known about the enzymes that the fungi produce in the infected grain. We have shown previously that one plant pathogenic fungus, Fusarium culmorum, produced subtilisin- and trypsin-like enzymes when grown in a cereal protein medium. To establish whether these proteinases were also synthesized in FHB-infested barley in vivo, field-grown barley was infested as the heads emerged. Extracts were prepared from the grain as it developed and matured and their proteolytic activities were measured with N-succinyl-Ala-Ala-Pro-Phe p -nitroanilide and N-benzoyl-Val-Gly-Arg p -nitroanilide. The heavily infested barleys contained both subtilisin- and trypsin-like activities. These enzymes reacted with antibodies prepared against each of the two F. culmorum proteinases, indicating that those produced in the laboratory cultures and in the field-infested barley were the same. The presence of these proteinases correlated with the degradation of specific buffer-soluble proteins in the infested grains. These enzymes readily hydrolyzed barley grain storage proteins (C- and D-hordeins) in vitro. The presence of these Fusarium proteinases in the barley indicates that they probably play an important role in the infestation, but exactly how and when they function is not clear. 相似文献
10.
Hamid Khazaei Kenneth Street Arja Santanen Abdallah Bari Frederick L. Stoddard 《Genetic Resources and Crop Evolution》2013,60(8):2343-2357
Drought is a major constraint to faba bean (Vicia faba L.) production, and there are many mechanisms by which leaves can regulate water loss. Our primary objective was to test if the origin of the faba bean accessions, from drought-prone and non-drought-prone environments, was associated with differences in measurable aspects of stomatal morphology and physiology related to water use. Two sets, each consisting of 201 faba bean accessions, were chosen from environments with contrasting seasonal moisture profiles following the focused identification of germplasm strategy (FIGS), and then screened under well watered conditions. From these, two subsets of 10 accessions each were chosen to test for differences in response to drought. Parameters related to stomatal function and water status were measured. The dry-adapted set had bigger stomata, higher leaf relative water content (LRWC) and cooler leaves under well watered conditions. Stomatal density and stomatal area per unit area of leaflet were negatively correlated with gas exchange parameters and positively correlated with intrinsic water use efficiency. Drought caused stomatal densities to increase in the dry set while stomatal length decreased in both sets. The moisture deficit was sufficient to decrease gas exchange in both sets to similar levels, but the dry-adapted set maintained warmer leaves and a higher LRWC that showed no significant correlations with leaf morphology or gas exchange, demonstrating more effective stomatal regulation. The results also support that collection site data from the environment where genetic resources are collected can be used as indicators of adaptive traits in an herbaceous annual species. 相似文献