Reproductive emergencies.

The emergency clinician is frequently called on to manage problems relating to the female reproductive tract. Because owners sel-dom have the medical knowledge needed to differentiate normal from abnormal reproductive behaviors, they frequently look to the emergency veterinarian for guidance and information during and after parturition. For this reason, it is essential that the veterinarian have a good understanding of the normal reproductive cycle as well as the common emergencies that may occur. This article reviews the events surrounding normal parturition in the dog and cat and the reproductive emergencies seen most commonly in practice.     

Genomic characterization of the first oral avian papillomavirus in a colony of breeding canaries (<Emphasis Type="Italic">Serinus canaria</Emphasis>)

Papillomaviruses are non-enveloped, DNA viruses that infect skin and mucosa of a wide variety of vertebrates, causing neoplasias or simply persisting asymptomatically. Avian papillomaviruses, with six fully sequenced genomes, are the second most studied group after mammalian papillomaviruses. In this study, we describe the first oral avian papillomavirus, detected in the tongue of a dead Yorkshire canary (Serinus canaria) and in oral swabs of the same bird and other two live canaries from an aviary in Madrid, Spain. Its genome is 8,071 bp and presents the canonical papillomavirus architecture with six early (E6, E7, E1, E9, E2, E4) and two late open reading frames (L1 and L2) and a long control region between L1 and E6. This new avian papillomavirus L1 gene shares a 64% pairwise identity with FcPV1 L1, so it has been classified as a new species (ScPV1) within the Ethapapillomavirus genus. Although the canary died after showing breathing problems, there is no evidence that the papillomavirus caused those symptoms so it could be part of the oral microbiota of the birds. Hence, future investigations are needed to evaluate the clinical relevance of the virus.     

树木根系分布与季节冻结滞水关系探讨

采用造林技术于冻结滞水理论技术相结合的方法,研究树木根系发育分布与冻结滞水的相关性,为防治荒漠,提高造林成活率提供理论技术依据,研究结果表明,苗木和高秆造林树木根系主要分布在地表以下20 cm~75 cm深度内,即冻结滞水富水带内,含水量达40%~50%,春季冻融水满足苗木生根成活的要求.80 cm~160 cm深度分贫水带,含水量〉5%,高杆造林树木根系极少.到160 cm~200 cm含水量增加,发育,第二层根系。冻结滞水资源是提高苗木成活率,生存繁的重要水资源。     

EIAV Rev蛋白拮抗eqTRIM5α介导的AP-1信号通路的机制研究

为探究马传染性贫血病毒(EIAV)附属蛋白Rev负调控Tripartite motif-containing protein 5α(TRIM5α)介导的AP-1信号通路的机制,本研究将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TRIM5α基因的质粒及pGL3-AP-1-Luc(AP-1报告质粒)共转染HEK 293T细胞,采用荧光素酶试验检测Rev对TRIM5α激活的AP-1信号通路的影响;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TAK1、TAB2、P38和c-Jun基因的质粒及pGL3-AP-1-Luc共转染HEK 293T细胞,采用荧光素酶试验检测Rev对TRIM5α下游转导分子(TAK1、TAB2、P38、c-Jun)激活的AP-1信号通路的影响;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含TAK1、TAB2、P38基因的质粒共转染HEK293T细胞,利用western blot试验分别检测TAK1、TAB2、P38的表达水平;将pEIAV-Rev-HA和pcDNA3.1质粒分别与含P38基因的质粒共转染HEK 293T细胞后加入蛋白酶体抑制剂MG132,利用western blot检测P38蛋白的表达情况。结果显示,共转染EIAV-Rev-HA实验组中TRIM5α对AP-1的激活倍数为0.4,而共转染pcDNA3.1对照组中相应的激活倍数为26.0;共转染pEIAV-Rev-HA实验组中,TAK1、TAB2、P38和c-Jun对AP-1信号通路的激活倍数分别为7.7、0.1、0.6、9.8,而共转染pcDNA3.1对照组中对AP-1信号通路的激活倍数分别为60.0、1.5、6.3、12.0;转染pEIAV-Rev-HA+pP38-Flag组与转染pcDNA3.1+pP38-Flag组相比,前者P38蛋白的表达量显著降低;加入蛋白酶体抑制剂组则恢复了P38蛋白的表达。上述结果表明,EIAV Rev显著下调eqTRIM5α及其下游转导分子TAK1、TAB2、P38激活的AP-1信号通路,但不显著下调c-Jun激活的AP-1信号通路;EIAV Rev通过蛋白酶体途径降解P38蛋白的表达而抑制eqTRIM5α激活的AP-1信号通路。本研究结果为理解EIAV与宿主蛋白相互作用提供参考依据。     

我国西北地区苜蓿种子产业化发展优势与对策

我国西北地区发展苜蓿种子产业不仅具有得天独厚的资源优势,而且还具有较好的经济效益和巨大的生产潜力。由于长期对苜蓿种子生产不重视,苜蓿栽培历史虽然悠久,但迄今苜蓿种子生产同有形成较大规模的产业,种子商品化极低,种子生产资源优势还没有转变为经济优势,只有加快苜蓿种子产业化步伐,才能改变目前西北地区苜蓿种子生产的落后局面。     

微细木质粉尘静电除尘器的设计

近年来,随着人们对环境质量的要求逐渐提高,木材工业除尘日益得到重视。众所周知,木材工业是产生粉尘的大户,如不采取有效的除尘措施,产生的粉尘不但会造成极大的环境污染,而且还会对工人的身体健康、产品质量、设备的精度和使用年限及生产的安全造成危害。目前,在木材工业中,广泛采用的多是离心力除     

磁化水在农作物上应用的研究进展

针对磁化水对农业生产的影响,简述了国内外关于磁化水应用的研究动态和发展趋势,介绍了磁化水在促进作物种子萌发、增强抗逆性以及提高产量和改善品质方面的研究进展。     

残膜回收工艺和收膜机构

介绍了田间残膜的特性及机械化收膜工艺、残膜同收机中常用收膜机构的种类及工作特性，为残膜回收机的研究设计及主要工作部件结构的优化组合提供参考。