Inorganic and organic carbon dynamics in forested soils developed on contrasting geology in Slovenia—a stable isotope approach

Purpose

Soil carbon dynamics were studied at four different forest stands developed on bedrocks with contrasting geology in Slovenia: one plot on magmatic granodiorite bedrock (IG), two plots on carbonate bedrock in the karstic-dinaric area (CC and CD), and one situated on Pleistocene coalluvial terraces (FGS).

Materials and methods

Throughfall (TF) and soil water were collected monthly at each location from June to November during 2005–2007. In soil water, the following parameters were determined: T, pH, total alkalinity, concentrations of Ca²⁺ and Mg²⁺, dissolved organic carbon (DOC), and Cl^? as well as δ¹³C_DIC. On the other hand, in TF, only the Cl^? content was measured. Soil and plant samples were also collected at forest stands, and stable isotope measurements were performed in soil and plant organic carbon and total nitrogen and in carbonate rocks. The obtained data were used to calculate the dissolved inorganic carbon (DIC) and DOC fluxes. Statistic analyses were carried out to compare sites of different lithologies, at different spatial and temporal scales.

Results and discussion

Decomposition of soil organic matter (SOM) controlled by the climate can explain the ¹³C and ¹⁵?N enrichment in SOM at CC, CD, and FGS, while the soil microbial biomass makes an important contribution to the SOM at IG. The loss of DOC at a soil depth of 5 cm was estimated at 1 mol m^?2 year^?1 and shows no significant differences among the study sites. The DOC fluxes were mainly controlled by physical factors, most notably sorption dynamics, and microbial–DOC relationships. The pH and pCO₂ of the soil solution controlled the DIC fluxes according to carbonate equilibrium reactions. An increased exchange between DIC and atmospheric air was observed for samples from non-carbonate subsoils (IG and FGS). In addition, higher δ¹³C_DIC values up to ?19.4?‰ in the shallow soil water were recorded during the summer as a consequence of isotopic fractionation induced by molecular diffusion of soil CO₂. The δ¹³C_DIC values also suggest that half of the DIC derives from soil CO₂ indicating that 2 to 5 mol m^?2 year^?1 of carbon is lost in the form of dissolved inorganic carbon at CC and CD after carbonate dissolution.

Conclusions

Major difference in soil carbon dynamics between the four forest ecosystems is a result of the combined influence of bedrock geology, soil texture, and the sources of SOM. Water flux was a critical parameter in quantifying carbon depletion rates in dissolved organic and inorganic carbon forms.

     

Influence of temperature and steam environment on set recovery of compressive deformation of wood

Low-density hybrid poplar wood (Populus deltoides?×?Populus trichocarpa) was densified by mechanical compression under saturated steam, superheated steam, and transient conditions at temperature levels of 150, 160, and 170°C. Furthermore, compression of wood under saturated steam conditions at 170°C, followed by post-heat-treatment at 200°C for 1, 2, and 3?min, was performed. To determine the influence of compression treatment on the set recovery, specimens were subjected to five cycles of water soaking and drying. Modulus of rupture (MOR) and modulus of elasticity (MOE) of specimens compressed under saturated steam conditions at 170°C and post-heat-treated at 200°C were determined in the dry condition and after five soak/dry cycles. Higher temperature of the compression treatment resulted in lower equilibrium moisture content, while the steam conditions during the treatment and the post-heat-treatment did not have significant effect. Furthermore, the highest degree of densification was obtained in specimens compressed under saturated steam conditions at 170°C and post-heat-treated at 200°C. The steam condition and temperature influenced the set recovery of compressive deformation. Reduced hygroscopicity does not necessarily imply reduced set recovery. The results established that considerable fixation of compressive deformation can be obtained by compressing the wood in a saturated steam environment and by post-heat-treatment at 200°C. The short heat-treatment had no influence on MOR or MOE, but soaking/drying treatments caused a decrease in the MOR and MOE.     

Physical carbon‐sequestration mechanisms under special consideration of soil wettability

The protective impact of aggregation on microbial degradation through separation has been described frequently, especially for biotically formed aggregates. However, to date little information exists on the effects of organic‐matter (OM) quantity and OM quality on physical protection, i.e., reduced degradability by microorganisms caused by physical factors. In the present paper, we hypothesize that soil wettability, which is significantly influenced by OM, may act as a key factor for OM stabilization as it controls the microbial accessibility for water, nutrients, and oxygen in three‐phase systems like soil. Based on this hypothesis, the first objective is to evaluate new findings on the organization of organo‐mineral complexes at the nanoscale as one of the processes creating water‐repellent coatings on mineral surfaces. The second objective is to quantify the degree of alteration of coated surfaces with regard to water repellence. We introduce a recently developed trial that combines FTIR spectra with contact‐angle data as the link between chemical composition of OM and the physical wetting behavior of soil particles. In addition to characterizing the wetting properties of OM coatings, we discuss the implications of water‐repellent surfaces for different physical protection mechanisms of OM. For typical minerals, the OM loading on mineral surfaces is patchy, whereas OM forms nanoscaled micro‐aggregates together with metal oxides and hydroxides and with layered clay minerals. Such small aggregates may efficiently stabilize OM against microbial decomposition. However, despite the patchy structure of OM coating, we observed a relation between the chemical composition of OM and wettability. A higher hydrophobicity of the OM appears to stabilize the organic C in soil, either caused by a specific reduced biodegradability of OM or indirectly caused by increased aggregate stability. In partly saturated nonaggregated soil, the specific distribution of the pore water appears to further affect the mineralization of OM as a function of wettability. We conclude that the wettability of OM, quantified by the contact angle, links the chemical structure of OM with a bundle of physical soil properties and that reduced wettability results in the stabilization of OM in soils.     

The effects of diet supplemented with Lactobacillus rhamnosus on tissue parameters of rainbow trout,Oncorhynchus mykiss (Walbaum)

This study was carried out to establish the effects of a 6 week treatment with the diet supplemented with L. rhamnosus in concentrations of 10⁷ CFU g^?1 (G1 group) and 10⁸ CFU g^?1 (G2 group) on the condition expressed by condition factors (Fulton's, Clark's and B), intestinal microbiology, haematological, histological and selected antioxidative parameters of rainbow trout. A significantly higher condition factors were found in G1 group indicating that higher concentration of probiotic (10⁸ CFU g^?1) did not result in the better condition. Cholesterol and urea levels were significantly higher in both G1 and G2 groups, albumin in G1 and creatinine in G2 group with respect to control. A significantly higher liver TBARS level was observed in G2 group. The feeding with supplemented probiont apparently changed the resident microbiota. Three weeks after withdrawal of the supplemented feed, the microflora mostly reverted to the control composition, although L. rhamnosus in faecal matter of fish remained inherent. The epithelial structure of the proximal and distal intestine revealed the increased absorptive area in both treated groups, as well as the increase in the mucin‐secreting goblet cells. The L. rhamnosus‐treated groups demonstrated the capacity for the augmentation of the innate host defence.     

Soil CO<Subscript>2</Subscript> sources above a subterranean cave—Pisani rov (Postojna Cave,Slovenia)

Purpose

The objective of this research is to detect abiotic sources of soil CO₂ above a subterranean cave in the Slovenian karst region.

Materials and methods

The research was performed in the forest above Pisani rov (Postojna Cave) near the town of Postojna (SW Slovenia) and also in the cave. Soil gas, atmospheric air and cave air carbon stable isotope composition (δ¹³C_CO2) and CO₂ concentration were measured. Sampling and measurements were performed bi-monthly at the test and control sites above the cave. The abiotic source of soil CO₂ was estimated using a stable isotope mass balance calculation.

Results and discussion

Similar seasonal patterns of soil CO₂ and δ¹³C_CO2 values were observed at both the test and control sites until spring, with higher levels of CO₂ observed in summer and lower in winter. The δ¹³C_CO2 showed the opposite trend, i.e. lower values (?26 to ?20 ‰) in summer and higher values (up to ?17 ‰) in winter and early spring. In spring, the soil CO₂ concentration decreases and the δ¹³C_CO2 value increases only at the control site. A time series of a modelled “isotopically light” endmember revealed large shifts in the data values, due to the presence of an abiotic CO₂ source. Results suggest that the subterranean CO₂ pool and its ventilation is the main source of soil CO₂, accounting for up to 80 % of the soil gas during cold periods.

Conclusions

Ventilation from subterranean cavities is an important source of soil CO₂ in karstic areas and should be taken into account during carbon cycling studies.

     

Chloroguaiacols Change Some Antioxidative Parameters and Affect the Activity of Glutathione S-transferase in the Leaves of Reed Canary Grass (Phalaris arudinacea)

In this work, we evaluated the effect of harmful ecotoxins, 4,5,6-trichloroguaicol (4,5,6-TCG) and tetrachloroguaiacol (TeCG), on the oxidation of the fluorescent probe dihydrorhodamine 123, the content of free phenols and the level of the total, oxidized and reduced glutathione in the leaves of reed canary grass (Phalaris arudinacea). Furthermore, the effect on the activity of guaiacol peroxidase and glutathione S-transferase was investigated. Both 4,5,6-TCG and TeCG increased the activity of guaiacol peroxidase and glutathione S-transferase, they also elevated the content of free phenols and the level of the total glutathione. A stronger effect was exerted by tetrachloroguaiacol, which strongly increased the level of the total glutathione and the content of free phenols on the 3rd and 6th day of the experiment. The activity of glutathione S-transferase was more intensively induced by trichloroguaiacol. Both 4,5,6-TCG and TeCG oxidized dihydrorodamine 123 and the effect was stronger in the presence of magnesium ions.     

Performance of three-layer composites with densified surface layers of Nothofagus pumilio and N. antarctica from Southern Patagonian forests

Nothofagus pumilio and N. antarctica forests of Southern Patagonia, Argentina are currently being managed for production of saw logs with fast growing conditions. The result of these management strategies is faster growing forests, but also an increase in the percentage of low-density wood. The motivation for this study was to find a way to valorise this low-density wood. Surface and bulk densification treatments were applied these wood species and were then applied as face layers in three-layer composites. The mechanical properties of three-layer composites were studied. The modulus of elasticity, modulus of rupture, and modulus of hardness were improved compared to many types of structural composite lumber. This increase in mechanical properties of N. pumilio and N. antarctica wood opens the possibility for its use in structural composites and added value to these otherwise underutilised and undervalued species.