伊朗里海森林山坡上生长的欧洲鹅耳根系分布和抗张强度研究

对生长在伊朗北部丘陵地带的8株欧洲鹅耳的根面积比率和根系拉伸强度进行了测定,评估了欧洲鹅耳根系生物力学特性。应用剖面挖掘法测得土层深度为0．1米的样品根面积比率。共用了123份根样品分析根系拉伸强度。结果表明,根面积比率随土层深度增加而减小,变化规律遵从幂函数;上坡面和下坡面根面积比率无统计差别。根系可达到的最大土层深度为0．75米,但是最大根面积比率存在于土层深度0．1米内。下坡面最小和最大根面积比率分别为0．004％和6．431％;而上坡面的根面积比率分别为0．004％和3．995％。上、下坡面根数无显著差异,而且90％以上的根系深入到50-60厘米的土层中。Spearman双变量相关性分析结果表明,根面积比率与根数无显著关系。平均根拉伸强度为31．51±1．05MPa,且根系直径越粗根拉伸强度越小,遵从幂函数变化。图5表1参38。     

Effects of weaning age and diets on ontogeny of digestive activities and structures of pikeperch (<Emphasis Type="Italic">Sander lucioperca</Emphasis>) larvae

Growth and ontogeny of digestive function were studied in pikeperch (Sander lucioperca) larvae weaned on artificial food at different ages. Three weaning treatments initiated respectively on day 9 (W9), day 15 (W15) or day 21 (W21) post-hatching (p.h.) were compared with a control group, fed Artemia nauplii from first feeding until the end of the rearing trial on day 36 p.h. The digestive enzyme activities and the ontogeny of digestive structures were investigated using enzymatic assays and histological methods. Growth of pikeperch larvae was significantly affected by precocious weaning. Pancreatic (trypsin and amylase) and intestinal (leucine-alanine peptidase, leucine aminopeptidase N and alkaline phosphatase) enzyme activities were detected from hatching onwards, increased at the moment of first feeding and then decreased. Pepsin secretion occurred at day 29 p. h. only, concurrently with the stomach development and differentiation of gastric glands. In the early weaning group (W9) the maturation process of intestinal enterocytes seems to be impaired and/or delayed and several signs of malnutrition were recorded. Except for alkaline phosphatase activity, no differences in enzyme activities and development of digestive structures were observed among the control, W21, and W15 groups. Moreover, at the end of the experiment, no differences in proteolytic activities were observed among larvae from the different treatments, indicating that, in surviving individuals, the digestive structures were properly developed and the larvae had acquired an adult mode of digestion. Based on the artificial diet used, our results suggested that pikeperch larvae can be weaned from day 15 p.h. without significant adverse effect on digestive capacities (except for alkaline phosphatase) or development of digestive tract, while earlier weaning impaired the onset of the maturation processes of the digestive system, both in terms of morphological structures and enzymatic activities.     

Hedysarum phylogeny mediated by RFLP analysis of nuclear ribosomal DNA – RFLP and Hedysarum phylogeny

The RFLP technique has been employed to investigate phylogenetic relationships between species of the Hedysarum genus. Homologous DNA probes were used to generate patterns from a set of the Mediterranean group. The degree of band sharing was used to estimate genetic distances between species and to draw phylogenetic trees. These are in good agreement with phylogeny based on morphological and isozyme markers, but contain novel insights. The results are discussed in the context of current work in molecular biosystematics in Hedysarum complex. Attempts made in this approach to access the extent of variability at the DNA level occurring with the domestication process have been investigated. With the availability of the tested probes, it has been assumed that the genetic structure is not affected by the domestication process.     

Ruminal and morphometric parameters of the rumen and intestines of sheep fed with increasing levels of spineless cactus (Nopalea cochenillifera Salm-Dyck)

Tropical Animal Health and Production - The objective of this study was to evaluate the ruminal parameters (pH, N-NH3, and microbial protein) and morphometry of the rumen and intestine of sheep fed...     

Recreational sandboxes for children and dogs can be a source of epidemic ribotypes of Clostridium difficile

Different studies have suggested that the sand of public playgrounds could have a role in the transmission of infections, particularly in children. Furthermore, free access of pets and other animals to the playgrounds might increase such a risk. We studied the presence of Clostridium difficile in 20 pairs of sandboxes for children and dogs located in different playgrounds within the Madrid region (Spain). Clostridium difficile isolation was performed by enrichment and selective culture procedures. The genetic (ribotype and amplified fragment length polymorphism [AFLP]) diversity and antibiotic susceptibility of isolates was also studied. Overall, 52.5% (21/40) of samples were positive for the presence of C. difficile. Eight of the 20 available isolates belonged to the toxigenic ribotypes 014 (n = 5) and 106 (n = 2), both regarded as epidemic, and CD047 (n = 1). The other 12 isolates were non‐toxigenic, and belonged to ribotypes 009 (n = 5), 039 (n = 4), and 067, 151 and CD048 (one isolate each). Nevertheless, all isolates (even those of a same ribotype) were classified into different AFLP genotypes indicating non‐relatedness. In conclusion, our results revealed the presence of epidemic ribotypes of C. difficile in children's and dog's sandboxes located nearby, which constitutes a major health risk.     

Molecular Genetic Analysis of the Variable Number of Tandem-Repeat Alleles at the Phenylalanine Hydroxylase Gene in Iranian Azeri Turkish Population

Background:

The variable numbers of tandem-repeat (VNTR) alleles at the phenylalanine hydroxylase (PAH) gene have been used in carrier detection and prenatal diagnosis in phenylketonuria families. This study was carried out to analyze VNTR alleles at the PAH gene in Iranian Azeri Turkish population.

Methods:

In this study, 200 alleles from general population were studied by PCR.

Results:

The frequencies of VNTR alleles were 45%, 46%, 2%, 3%, 1%, and 3% in studied group regarding 3, 8, 9, 11, 12, and 13 repeat copies, respectively. Statistically significant differences were not found between expected and observed frequencies of VNTR genotypes (P > 0.05).

Conclusions:

VNTR alleles with three and eight repeats were frequent, and the VNTR alleles with 13 repeats showed 3% frequency in the tested group. This study is the first report on tested population genetic structure using VNTR alleles at the PAH gene. Key Words: Phenylalanine hydroxylase, Population genetics, Variable numbers of tandem-repeat     

How does organic matter affect the physical and mechanical properties of forest soil?

Determining the physical and mechanical properties of soil and its behavior for engineering projects is essential for road construction operations. One of the most important principles in forest road construction, which is usually neglected, is to avoid mixing organic matter with road materials during excavation and embankment construction. The current study aimed to assess the influence of organic matter on the physical properties and mechanical behaviors of forest soil and to analyze the relation between the amount of organic matter and the behavior of forest soil as road material. A typical soil sample from the study area was collected beside a newly constructed roadbed. The soil was mixed with different percentages of organic matter(control treatment, 5, 10, and 15% by mass) and different tests including Atterberg limits, standard compaction, and California bearing ratio(CBR) tests were conducted on these different soil mixtures. The results showed that soil plasticity increased linearly with increasing organic matter.Increasing the organic matter from 0%(control) to 15%resulted in an increase of 11.64% of the plastic limit and 15.22% of the liquid limit after drying at 110 ℃. Also,increasing the organic matter content reduced the soil maximum dry density and increased the optimum moisture content. Increasing the organic matter from 0 to 15% resulted in an increase of 11.0% of the optimum moisture content and a decrease of 0.29 g/cm~3 of the maximum dry density. Organic matter decreased the CBR, which is used as the index of road strength. Adding 15% organic matter to the soil resulted in a decrease of the CBR from 15.72 to 4.75%. There was a significant difference between the two drying temperatures(60 and 110 ℃) for the same organic matter mixtures with lower water content values after drying at 60 ℃. The results revealed the adverse influence of organic matter on soil engineering properties and showed the importance of organic matter removal before excavation and fill construction.     

Agricultural zinc fluxes into soils and crops of central Iran at regional scale

Mass flux assessment can provide information that is essential for a sustainable management of elements in agricultural soils. In this article, we present an assessment of regional-scale averages of zinc (Zn) fluxes into agricultural soils and crops of central Iran for the period 1997–2011, using available databases such as regional agricultural statistics. The basic units of the balances were 15 townships of the provinces Qom, Isfahan and Fars. Averaged over the entire study region, the net Zn input into arable soil resulting from all fertilizer inputs – Zn removal with harvested crops was 1515 g ha^?1 yr^?1 across the entire region, with a range of 438–3009 g ha^?1 yr^?1 among townships. Estimated average Zn inputs with manure, mineral fertilizers, sewage sludge and compost were 1254, 531, 19 and 7 g ha^?1 yr^?1, respectively. The input-to-output ratio of these fluxes ranged from 1.8 to 12.9 among townships and averaged 6.1 for the entire study area. Considering that outputs other than with crop harvests are minor, Zn stocks are rapidly building up in the soils of the study region. Uncertainties in the manure and crop removal data were the main sources of estimation uncertainty in this study.