JEV、PPV、PRRSV、PRV多联PCR的应用研究

用 JEV、PRRSV二联 PCR,PPV、PRV二联 PCR以及这 4种病毒 4联 PCR对来自内蒙、广州、广西、天津、北京、吉林病料进行检测 ,共检了 1 4 6份病料。其中 PRRSV阳性 7份 ,PPV阳性 1 2份 ,PRV阳性 2 1份 ,PRV和 PPV混合感染 4份。并对 5份人工接种 JEV小鼠病料检测 ,其中 4份为阳性。随后对部分阳性 PCR扩增产物进行点杂交和核苷酸测序鉴定 ,证实了 PCR扩增准确性。对内蒙 PRRSV阳性扩增带测序结果显示 ,我国流行 PRRSV为美洲型 ,在扩增片段的核苷酸序列上有 3个碱基差异     

猪化脓隐秘杆菌的分离鉴定

<正>化脓隐秘杆菌(Trueperella pyogenes)常引起牛、羊、猪等动物的化脓性感染,主要表现为肺炎、关节炎、心内膜炎、乳腺炎、皮下脓肿等〔1〕,给养殖业带来较大的经济损失。化脓隐秘杆菌以前被称为化脓棒状杆菌、化脓放线菌,1997年Pascual Ramos等〔2〕将该菌从放线杆菌属中重新分类为隐秘杆菌属。化脓隐秘杆菌是隐秘杆菌属中致病力最强的病原体,是经济型家畜(牛、羊和猪)的一种     

近33年闽西地区霜的气候变化特征

利用闽西地区7个气象观测站的观测资料建立时间序列,运用趋势分析、相关分析等方法研究分析1981-2013年闽西地区霜的气候变化特征。结果表明:初霜期推迟,终霜期提前,无霜期延长,近33年约增加22天。这为农业生产的调整和布局提供了一定的参考依据。     

高蛋白大豆新品种绥农76的选育与推广

绥农76是黑龙江省农业科学院绥化分院以绥07-1186为母本、绥07-104为父本,经过常规人工杂交选育而成的高蛋白大豆新品种。经农业农村部谷物品质监督检验测试中心(哈尔滨)检测,绥农76粗蛋白质(干基)含量高达46.78%。2019年通过黑龙江省农作物品种审定委员会审定(审定编号:黑审豆2019021)。该品种生育日数115 d,适于黑龙江省第三积温带及吉林、内蒙、新疆等相应区域种植。     

Nutrient uptake requirements with increasing grain yield for rice in China

Improved estimates of nutrient requirements for rice（Oryza sativa L.）in China are essential to optimize fertilization regulation for increasing grain yields and reducing the potential of environmental negative influences,especially under high-yielding intensive systems.A database involving rice grain yields,nutrient concentrations and accumulations collected from on-field station experiments in the literatures published from 2000 to 2013 in China was developed to understand the relationships between grain yields and plant nutrient uptakes,and to quantify nutrient requirements for different yield levels.Considering all data sets,rice grain yield ranged from 1.4 to 15.2t ha^-1with the mean value of 7.84t ha^-1,and ca.10.4%of yield observations were higher than the yield barrier level of 10 t ha^-1.N requirement to produce one ton grain was 21.10 kg for the yield range<4.0t ha^-1with a high variation of 45.8%.Except of the yield range<4.0t ha^-1,the values of N requirement,firstly increased from 18.78 kg for yield range 4.0-5.5 t ha^-1to 20.62 kg for yield range 7.0-8.5 t ha^-1,then decreased slightly to 19.67 and19.17 kg for the yield range 8.5-10 and>10 t ha^-1,respectively.Phosphorus（P）and potassium（K）requirements showed increasing trends,from 3.51 and 19.87 kg per t grain for<4.0 t ha^-1yield range to 4.10 and 21.70 kg for>10.0t ha^-1range.In conclusion,nutrient requirement varied with increasement of grain yield,and N,P and K presented various response trends,increasing,declining or stagnating,which would be of great benefit for improving fertilizer strategies.     

Effects of CpG-B ODN on the protein expression profile of swine PBMC

The CpG motif within bacterial DNA is a potent immuno-stimulatory moiety. Here, using a 2-D electrophoretic approach, we investigated the effect of synthetic oligodeoxynucleotide containing a B type CpG motif (CpG-B ODN) on the protein expression profile of swine peripheral blood mononuclear cells (PBMC). We found that several proteins including spondin 1, N-acetolactate alpha linked acidic dipeptidase; V kappa light chain, T cell receptor variable alpha chain, heat shock protein (Hsp) 60, Hsp70, KIAA0857 protein, and PNAS-146 were up-regulated in PBMC by CpG-B ODN stimulation. Further studies showed that CpG-B ODN-mediated Hsp60, Hsp70 and Hsp90 expressions were closely associated with the TLR9 signalling pathway. Pretreatment with inhibitors of Hsp70, Hsp90 and TLR9 all blocked the CpG-B ODN-mediated anti-apoptotic effect in swine PBMC. These results suggest that CpG-B ODN treatment of swine PBMC may enhance the expression of biologically active proteins, notably spondin 1, V kappa light chain, T cell receptor variable alpha chain and Hsps, which may play an important role in CpG-B ODN-mediated activation of immune responses and enhancement of swine PBMC survival.     

DESIGN OF Ada-SUB COMPILER AND REALIZE METHOD WITH YACC OF MP/M-86

The lexical and syntex of Ada-sub and the compiler of Ada-sub and the method to realize it designed by us with the tool of YACC efficacious exploit on the Aim-l6 micro computer are discussed in this paper.     

Advances in Pharmacological Studies of Coptis Chinesis

A Coptis Chinesis is the dry rhizoma of ranunculaceous Coptis Chinesis Franch or Coptis deltoidea C. 7. Cheng et Hisao, or Coptis teeta Wall. It is the traditional Chinese herb, which is used in the contagious diseases. In recent years, its new pharmacological actions have been reported, which are antitumour, hypoglycemic response, immunoloregulation, improving the functions of cardiovascular. The important pharmacological functions of Coptis Chinesis are hypoglycemic response, improving the functions of cardiovascular, antitumour. With the deeply researching of Coptis Chinesis pharmacology, and the continuous founding of pharmacological mechanism, new preparation will be widely applied in the clinic.