Soybean (Glycine max) Extracts Impact on Plant and Soil Biology

The aim of the present investigation was to determine the phytotoxic effect of aqueous extract from fresh and oven-dried leaves and roots of soybean plants on maize growth and on rhizosphere soil. The extract prepared from fresh leaves of drought-stressed soybean plants significantly increased the accumulation of proline, soluble sugar, and endogenous abscisic acid (ABA) content of maize-treated plants. Increase in antioxidant activity like superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), and endogenous ABA was recorded in treated plants. The soybean leaf extracts prepared from drought-stressed plants significantly decreased the soil pH and electrical conductivity (EC) of maize cultivated soil. The soybean drought-stressed leaf extracts prepared plants were found more effective in modulating the physiology of maize, indicating the higher allelopathic potential of soybean.     

Effects of Root Plant Growth Promoting Rhizobacteria Inoculations on the Growth and Nutrient Content of Grapevine

The objective of this study was to evaluate the effects of seven nitrogen (N₂)-fixing and/or phosphorus (P)-solubilizing and siderophore-producing microorganism based bio-fertilizers in single and triple strain combinations isolated from the acidic rhizospheric soil of native tea, grapevine, and wild red raspberries. As a result of this study, bacterial efficiency was found to be variable and depended on the bacterial strains and evaluated growth parameters. Plant growth-promoting rhizobacteria (PGPR) has improved macro- and micro-nutrient concentrations in grapevine leaves, and stimulated plant growth. Triple inoculation and single inoculation based bio fertilizers were found to stimulate overall plant growth, including shoot and leaf weight, main shoot length, leaf ground index, chlorophyll, nitrogen, zinc and iron content of grapevine cv ‘Italy’. Bio-fertilizers increased the nutrients such as nitrogen, zinc and iron concentrations and consequently increased the chlorophyll content of the leaves.     

Pathogenicity,Morpho-Species and Mating Types of <Emphasis Type="Italic">Alternaria</Emphasis> spp. causing Alternaria blight in <Emphasis Type="Italic">Pistacia</Emphasis> spp. in Turkey

Alternaria genus includes many plant pathogens on numerous hosts, causing leaf spots, rots and blights. Alternaria blight has been observed as one of the important fungal diseases of pistachio (Pistacia vera L.) as well as its wild relatives (P. terebinthus, P. lentiscus, P. khinjuk, P. atlantica, P. mutica) in Turkey. Alternaria species were sampled from Pistacia spp. hosts from different geographic regions in Turkey during field trips in late spring to early fall of 2013. Alternaria blight symptoms were observed mainly on fruits and rarely on leaves. Four hundred and twenty two of the isolates were morphologically defined as A. alternata, A. tenuissima, A. arborescens and also intermediate morpho-species between A. alternata/A. arborescens. Pathogenicity of the isolates was confirmed with host inoculations on detached fruits. Mating types of 270 isolates of Alternaria spp. from the collection were identified using a PCR-based mating type assay that amplifies either a MAT1-1 or a MAT1-2 fragment from the mating locus. Although a strongly clonal population structure was expected due to the putative asexual reproduction of these fungi, both idiomorphs were detected at equal frequencies at several different spatial scales. The distribution of mating types within each geographic region, within host species as well as in overall collection was not significantly different from 1:1. Amplified fragments of partial idiomorph sequences were obtained for representative isolates. Parsimony trees were depicted based on sequence data of mating type genes for these representative isolates as well as some other Alternaria species obtained by Genebank. Several point mutations presented a few clusters which are supported by high bootsrapped values. The Alternaria blight disease agents both from cultivated and wild hosts were pathogenic on pistachio which may cause difficulties to control the disease because of extensity of pathogen sources. Besides, equal mating type distribution of the pathogen at both geographic and host species levels suggests a potential for sexual reproduction of Alternaria spp. in Turkey.     

Forage qualities,forage yields and seed yields of some legume–triticale mixtures under rainfed conditions

Triticale (Triticosecale Wittmack) grown with legume has a better forage quality and greater yield potential than triticale grown alone. The objective of the study was to determine the suitable mixture rate of legume and triticale grown under the rainfed conditions in the northeast of Turkey. Field experiments, designed in a factorial randomized complete block with three replications, were carried out during 1998–1999 and 1999–2000 starting in the first week of November, 1998 and 1999. The highest dry matter yield (10.96 t ha^?1) was obtained from the mixture including 50% Hungarian vetch (Vicia pannonica Crantz.) and 50% triticale (Triticosecale Wittmack). Decreasing the seed rate of triticale in mixtures decreased dry matter yield while it increased the crude protein concentration of the hay mixture. The mixtures of 50% grasspea line 38 (Lathyrus sativus L.) and 50% triticale (Triticosecale Wittmack) and 50% hairy vetch and 50% triticale produced the highest seed and crude protein yield. Similarly, 50% Hungarian vetch (Vicia pannonica Crantz.) and 50% triticale (Triticosecale Wittmack) mixture produced the highest crude fiber and ash yield. Pure hairy vetch (Vicia villosa Roth.) and grasspea line 38 (Lathyrus sativus L.) yielded the maximum amount of NO₃ ^? -N to soil, and the highest plant concentration of crude protein, respectively. The mixtures outyielded the pure sowings with respect to dry matter (RYT=1.58) and grain yield (RYT=1.76).     

Bacterial leaf and peduncle soft rot caused byPectobacterium carotovorum on tulips in Konya, Turkey

The etiology of a new soft rot disease of tulips, causing leaf spots, leaf blight, neck and bulb rot in Konya, Turkey, was investigated. This disease occurred in tulip fields grown for seed bulbs of various varieties in 2002. Bacteria were isolated from the lesions on leaf, bud neck and bulbs and the causal bacterium was identified asPectobacterium carotovorum on the basis of biochemical and physiological tests. Cells were Gram-negative, rod-shaped, fermentative, potato-rot positive. Colonies were capable of growth at 37°C. On nutrient agar the colonies were creamy-white. The isolates were non-fluorescent on King’s B medium, positive for acetoin production, gelatin liquefaction, acid-production-from-lactose, and catalase; and negative for gas from glucose, reducing substances from sucrose, and phosphatase activity. Additionally, tests for egg yolk (lecithin), sensitivity to erythromycin, and pigmentation on yeast dextrose carbonate agar were negative; growth on 5% sodium chloride was positive. All the bacterial isolates obtained from the leaf, bud neck and bulbs produced the original symptoms following inoculation to the susceptible tulip variety ‘Gander’. The rate of damage caused by this bacterium was evaluated on several tulip varieties under field and storage conditions. Gander was the most susceptible variety in the field whereas ‘Salmon Parrot’ exhibited the highest rate of bulb rot in storage. Disease severity was lower in 2003 than 2002. http://www.phytoparasitica.org posting May 14, 2006.     

Thermal treatment of electrical poles

Thermal treatment is used to preserve the wood without any addition of any toxic chemicals. This process increases the dimensional stability and darkens the color of the wood. The improvement of the resistance to decay of wood by thermal treatment is also often suggested in the literature. However, some latest works contested if the durability of heat-treated wood is improved when it is used in contact with ground. The objective of this study was to investigate the possibility of thermally treating electrical poles which are larger compared to the standard wood lumber. One of the applications for thermally treated wood poles could be their use in environmental sensitive areas (along rivers, for example) as a replacement for untreated western red cedar (WRC) poles which are more expensive. Green and pre-dried red pine (Pinus resinosa) and jack pine (Pinus banksiana) poles, both with circular and square cross-sections, were heated to high temperatures under humid and inert atmosphere. Operating parameters such as maximum treatment temperature, maintenance time at this temperature, heating rate and gas humidity were varied in order to find most suitable treatment conditions for the poles. The tests showed that most of the cracks are formed during the drying process while thermal treatment only widened already existing cracks. The circular shape seems to promote crack formation during the drying period since the directional dependence of shrinking creates more stresses in circular poles compared to the square poles. A slight decrease in flexibility of the wood with increasing temperatures was observed. The protecting effect of gas humidity against oxidation of wood and the importance of the application of a moderate heating rate for poles with large cross-sections are also demonstrated in this article. The impact of the heat treatment on the resistance to decay of electrical wood pole will be presented in a future publication.     

Efficient Photocatalytic Reduction of CO<Subscript>2</Subscript> Present in Seawater into Methanol over Cu/C-Co-Doped TiO<Subscript>2</Subscript> Nanocatalyst Under UV and Natural Sunlight

Photocatalytic reduction of CO₂ in seawater into chemical fuel, methanol (CH₃OH), was achieved over Cu/C-co-doped TiO₂ nanoparticles under UV and natural sunlight. Photocatalysts with different Cu loadings (0, 0.5, 1, 3, 5, and 7 wt%) were synthesized by the sol–gel method and were characterized by XRD, SEM, UV–Vis, FTIR, and XPS. Co-doping with C and Cu into TiO₂ remarkably promoted the photocatalytic production of CH₃OH. This improvement was attributed to lowering of bandgap energy, specific catalytic effect of Cu for CH₃OH formation, and the minimization of photo-generated carrier recombination. Co-doped TiO₂ with 3.0 wt% Cu was found to be the most active catalyst, giving a maximum methanol yield rate of 577 μmol g-cat^?1 h^?1 under illumination of UV light, which is 5.3-fold higher than the production rate over C-TiO₂ and 7.4 times the amount produced using Degussa P25 TiO₂. Under natural sunlight, the maximum rate of the photocatalytic production of CH₃OH using 3.0 wt% Cu/C-TiO₂ was found to be 188 μmol g-cat^?1 h^?1, which is 2.24 times higher than that of C-TiO₂, whereas, no CH₃OH was observed for P25.     

Effect of Different Nitrogen Doses on Dry Matter Ratio,Chlorophyll and Macro/Micro Nutrient Content in Sweet Herb (Stevia rebaudiana Bertoni)

With this study, the effect of different nitrogen (N) doses applied to sweet herb (0, 50, 100, 150 and 200 N kg ha^?1) on the macro/micro nutrient element content, dry matter ratio and on the chlorophyll amount in the plant leaves is examined. The research was carried out in the research and trial field of Faculty of Agriculture Agronomy department, Akdeniz University, during the 2012–2013 growing season under field conditions for 2 years with four replications according to the randomized block trial design. Chlorophyll amounts were determined and the average was obtained with three readings from each leaf by a chlorophyll meter when the plants were in the beginning of flowering. Post-harvest macro and micro nutrient elements of the sweet herb were also investigated. According to the obtained data, while the highest chlorophyll and N amounts in the leaf for both the first and second years were determined as 46.97 SPAD (Minolta Corp, NJ, USA) and 1.34 N% 44.9 SPAD, 1.42 N%, respectively, under 200 kg ha^?1 N application, the lowest chlorophyll and N amounts were determined in the control group. In addition, N application in different doses for both years increased the phosphorus (P) and potassium (K) contents and increased the microelement contents, especially in the second year. At the same time, a correlation between the chlorophyll content in the plant leaves and the N amount was determined and a linear increase was observed in the chlorophyll amount with the increased green part.     

Performance and gastro-intestinal response of broiler chickens fed on cereal grain-based foods soaked in water.

1. Two experiments were carried out to investigate the addition of 1 3 kg water per kg air-dry mash diets containing high proportions (600 to 700 g/kg) of ground cereal grains (wheat, barley or oats) on broiler performance and the structure and function of the gastro-intestinal tract. 2. Chicks at the age of 7 d were fed on the wheat-, barley- or oats-based diets in the dry or wet forms for 35 d. Food and water intakes were recorded daily while body weight was measured weekly. Two birds from each treatment were killed each week to measure gut size and the viscosity of gut contents. Tissue samples from various digestive segments were histo-morphologically examined to determine the thickness of tissue layers, size of tissue glands, villa heights, crypt depths and thickness of tunica muscularis. Crypt cell proliferation rate (CCPR) for each segment was also determined using a metaphase arrest technique. 3. The results from both experiments showed that wetting food significantly (P<0.05) increased food intake, total water intake and body weight gain of broiler chickens. The body weight gains of birds were proportional to their food intakes so that the efficiency of food utilisation was similar for all treatments. Dry matter retention of food tended to increase in birds given wet food from 7 to 21 d but not thereafter, compared to the dry-fed birds. Although water intake from the water bottle was significantly (P<0.05) reduced in birds given wet food, total water intakes from the water bottle plus that from food were significantly (P<0.05) higher in the wet-fed birds than in the dry-fed birds. The ratio of total water to dry food intake was, however, similar in both feeding regimens. 4. The fresh empty weight of the gut was increased by wet-feeding while its relative weight to body weight and the length of gut was not affected by dietary treatments. Significantly greater development of the tissue glands in the proventriculus and gizzard was observed in the birds given wet food; this was associated with the reduced thickness of the muscular layer of these segments. An increase in villus height was also observed in duodenum, small intestine, caeca and colon of birds given wet food, compared to those given dry food. CCPR was significantly (P<0.05) reduced by wet-feeding throughout the digestive tract. This was associated with a significant decrease in the mean viscosity of the gut contents and the concentration of volatile fatty acids (VFAs) in the caeca. 5. Wetting diets based on cereal grains caused a significant improvement in the performance of broiler chickens. The mechanism of the beneficial effects of wet feeding could be attributed to the decreased viscosity of gut contents; the greater development of the layer of villi in the digestive segments and the reduced CCPR in the crypts of the epithelium.     

Evaluation of a crop water stress index for irrigation scheduling of bermudagrass

This study was conducted to assess crop water stress index (CWSI) of bermudagrass used widely on the recreational sites of the Mediterranean Region and to study the possibilities of utilization of infrared thermometry to schedule irrigation of bermudagrass. Four different irrigation treatments were examined: 100% (I₁), 75% (I₂), 50% (I₃), and 25% (I₄) of the evaporation measured in a Class A pan. In addition, a non-irrigated treatment was set up to determine CWSI values. The status of soil water content and pressure was monitored using a neutron probe and tensiometers. Meanwhile the canopy temperature of bermudagrass was measured with the infrared thermometry. The empirical method was used to compute the CWSI values. In this study, the visual quality of bermudagrass was monitored seasonally using a color scale. The best visual quality was obtained from I₁ and I₂ treatments. Average seasonal CWSI values were determined as 0.086, 0.102, 0.165, and 0.394 for I₁, I₂, I₃, and I₄ irrigation treatments, respectively, and 0.899 for non-irrigated plot. An empirical non-linear equation, Qave=1+⌊6[1+(4.853 CWSIave)^2.27]^−0.559⌋$Q_{\text{ave}}=1+⌊6{[1+{(4.853\text{CWS}{\text{I}}_{\text{ave}})}^{2.27}]}^{-0.559}⌋$, was deduced by fitting to measured data to find a relation between quality and average seasonal CWSI values. It was concluded that the CWSI could be used as a criterion for irrigation timing of bermudagrass. An acceptable color quality could be sustained seasonally if the CWSI value can be kept about 0.10.