Scanning electron microscopic studies of the kidney glomerulus of Pudu pudu (Molina, 1782), and a comparison with that of cattle, goats, sheep and angora rabbits

The kidney's microvascularization of the Pudu pudu is mostly similar to that of domestic animals. The renal parenchymatous arteries do not give off capsular branches. The majority of the Pudu pudu's glomerula shows spherical shape. Glomerula next to the medulla have a diameter which is an average of 25 microns larger than the diameter of those situated more peripherally. Their volume is comparable to that of the angora rabbit. The capillaries form anastomosis in the inner and outer part of the glomerulus.     

Jojoba seed meal proteins associated with proteolytic and protease inhibitory activities

The jojoba, Simmondsia chinensis, is a characteristic desert plant native to the Sonoran desert. The jojoba meal after oil extraction is rich in protein. The major jojoba proteins were albumins (79%) and globulins (21%), which have similar amino acid compositions and also showed a labile thrombin-inhibitory activity. SDS-PAGE showed two major proteins at 50 kDa and 25 kDa both in the albumins and in the globulins. The 25 kDa protein has trypsin- and chymotrypsin-inhibitory activities. In vitro digestibility of the globulins and albumins resembled that of casein and soybean protein concentrates and was increased after heat treatment. The increased digestibility achieved by boiling may be attributed to inactivation of the protease inhibitors and denaturation of proteins.     

Genetic variability in Turkmen populations of Pistacia vera L.

Seedlings of Pistacia vera L. developed from seeds of two separate populations in Turkmenistan, Kepele and Agachli, were evaluated for their growth potential and genetic polymorphism. Plant growth rate as well as random amplified polymorphic DNA (RAPD) analysis showed distinct differences between the two populations. In plant height growth rate, 17 Agachli accessions were 1.3 times higher on average than that of 10 accessions of Kepele (significant at p = 0.046) and 1.2 times higher for trunk diameter growth rate (p = 0.062). Cluster analysis divided most accessions into two main genetic groups according to their geographic origin. The Agachli group was further divided into two subgroups. One Kepele accession (K4), was genetically different from the rest and clustered on a separate outgroup. Two Agachli accessions (A12 and A17) were outside the two main populations clusters. Accessions K9 and K10 from Kepele were exceptional and were clustered in each of the two Agachli's subgroups, indicating a close genetic relationship between the two populations. In addition, high similarity values (0.58–1.00) and small genetic distances reflect plausible gene flow between Kepele and Agachli, which are 100 Km apart. Mantel test revealed significant relationship between the RAPD and the morphological traits matrices, pointing to the genetic basis for the measured differences in the growth rate. Growing the accessions on the same plot, under similar conditions enabled the evaluation of genotypic differences. The combination of morphological traits and molecular markers will further assist in preservation of genetic variability and cultivation of useful genotypes of P. vera L.     

Forest structure, composition, and tree diversity response to a gradient of regeneration harvests in the mid-Cumberland Plateau escarpment region, USA

Upland hardwood stands on mesic, escarpment-oriented sites on the Cumberland Plateau region of northeastern Alabama provide a myriad management opportunities. Stands are primarily managed for Quercus, but the high species diversity allows for management that targets multiple species. Stand composition is unique in that dominant species include shade tolerant species such as Acer saccharum, intermediate tolerant Quercus spp. and Carya spp., and intolerant Liriodendron tulipifera. Three replications of five levels of disturbance were created to assess species compositional changes; disturbances included three levels of harvest intensity, a mid-story herbicide treatment, and a control. After eight growing seasons, there were no discernable differences in species richness, diversity or evenness. Importance values based on relative basal area and relative density also changed little, except for clearcuts where L. tulipifera greatly increased. An initial gradient in basal area, canopy cover, and light created by harvesting or thinning dissipated following five growing seasons. Options exist for future stand management, including promoting two-aged or uneven-aged systems. Maintenance of Quercus will require additional tending.     

Molecular phylogenetics of mastodon and Tyrannosaurus rex

We report a molecular phylogeny for a nonavian dinosaur, extending our knowledge of trait evolution within nonavian dinosaurs into the macromolecular level of biological organization. Fragments of collagen alpha1(I) and alpha2(I) proteins extracted from fossil bones of Tyrannosaurus rex and Mammut americanum (mastodon) were analyzed with a variety of phylogenetic methods. Despite missing sequence data, the mastodon groups with elephant and the T. rex groups with birds, consistent with predictions based on genetic and morphological data for mastodon and on morphological data for T. rex. Our findings suggest that molecular data from long-extinct organisms may have the potential for resolving relationships at critical areas in the vertebrate evolutionary tree that have, so far, been phylogenetically intractable.     

Soft-tissue vessels and cellular preservation in Tyrannosaurus rex

Soft tissues are preserved within hindlimb elements of Tyrannosaurus rex (Museum of the Rockies specimen 1125). Removal of the mineral phase reveals transparent, flexible, hollow blood vessels containing small round microstructures that can be expressed from the vessels into solution. Some regions of the demineralized bone matrix are highly fibrous, and the matrix possesses elasticity and resilience. Three populations of microstructures have cell-like morphology. Thus, some dinosaurian soft tissues may retain some of their original flexibility, elasticity, and resilience.     

Modelling dynamics of ecosystem services basket in Mediterranean landscapes: a tool for rational management

Natural ecosystems are life-supporting systems providing diverse ecosystem services (ESs) and benefits to human societies: e.g., food and clean water, recreation opportunities or climate regulation. The contribution of natural and semi-natural ecosystems to the provision of such services depends to a large extent on vegetation structure and composition, which, in turn, change as a result of interactions between human decisions about land management, and spontaneous biological and environmental processes. Rational management of these dynamic ecosystems requires an ability to predict short- and long-term effects of management decisions on the desired ESs. The vegetation then contributes to, and modifies, the products and services obtained from the land. We applied mathematical modeling to study these complex relationships. We developed a model for a Mediterranean ecosystem which predicts the dynamics of multiple services in response to management scenarios, mediated by vegetation changes. Six representative ESs representing different groups were selected, based on available scientific information, for a detailed study: (1) density of geophytes, (2) potential contribution to honey production, (3) energy density of fleshy fruits foraged by birds, (4) forage for goats, (5) forage for cattle, and (6) carbon retention in woody plants. Mean contributions to each service by different vegetation cover types were estimated, and the overall service provided by the site was calculated as a weighted mean of these contributions. Services were measured in their appropriate units and subsequently standardized to a percentage of the maximum value observed in the study area. We attempted to combine all studied ESs, despite their different nature, into one “ESs basket”. This paper presents the dynamics of simulated vegetation composition and values of services in response to management scenarios involving grazing, fire and their combinations. Our approach can help land managers to evaluate alternative management scenarios by presenting the “services basket” obtained from the entire managed area.     

Underplanting to sustain future stocking of oak (Quercus) in temperate deciduous forests

New Forests - Oaks (Quercus spp.) are one of the most important tree taxa in the northern hemisphere. Although they are dominant in mixed species forests and widely distributed, there are frequent...