光箨篌竹笋期生长规律初步研究

本文对光箨篌竹笋期的出笋规律、成竹规律以及竹笋的生长发育规律进行了研究。初步认为,光箨篌竹林分的母竹立竹度一般应保持在2500株/亩左右、Ⅰ～Ⅱ级母竹株数保持在40％以上时,才能保证林分的优质高产与稳产;抽枝成竹历期长短受竹笋笋级大小、出土早晚或立地环境条件的影响可相应延长或推迟2～4d左右,一般历时25d,竹笋开始抽枝成竹时的高度一般为5m左右;竹笋在生长发育高峰期明显受到气候因子的影响,尤其是对气温更为敏感。     

熊蜂为温室茄子授粉试验

通过对温室茄子应用熊蜂授粉、人工蘸花授粉和空白对照的比较研究,结果表明熊蜂组的座果数比人工组和对照组分别增加了17.84%和33.32%,产量比人工组和对照组分别提高了27.93%和41.98%,果实含糖量比人工组和对照组分别增加了18.33%和21.16%,而且,熊蜂组的果实大而充实,商品性较好.说明利用熊蜂为温室茄子授粉,不仅能够促进座果,提高产量,而且可以改善果实品质,提升产品的附加值.     

Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuang long-Jie gu Pill (SLJGP) containing serum osteoblast proliferation

AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A.     

利用录音软件Nuendo演示拍音现象

目的 更方便快捷地演示拍音现象.方法 基于录音软件Nuendo,在两个虚拟的Input BUS(输入总线)上加载插件Test Generator,产生两列相干波,对应录入音轨一、二;同时两者叠加产生拍音信号,录入音轨三,并从音箱放出.观察干涉波形,验证相应关系.结果 验证并动态演示了拍音出现的条件以及拍频与两组相干波频率差的关系.结论 波形图像直观,操作简单,适合教师在多媒体教室演示.     

Isolation and Characterization of Efficient Active Compounds Using High-Performance Centrifugal Partition Chromatography (CPC) from Anti-Inflammatory Activity Fraction of Ecklonia maxima in South Africa

Ecklonia maxima is a brown seaweed, which is abundantly distributed in South Africa. This study investigated an efficient approach using high-performance centrifugal partition chromatography (HPCPC), which has been successfully developed for the isolation and purification of phlorotannins, eckmaxol, and dieckol from the ethyl acetate fraction of E. maxima (EEM). We evaluated EEM for its inhibitory effect against lipopolysaccharide (LPS)-induced inflammatory responses in zebrafish embryos. The separation of eckmaxol and dieckol from samples of EEM using HPCPC was found to be of high purity and yield under an optimal solvent system composed of n-hexane:ethyl acetate:methanol:water (2:7:3:7, v/v/v/v). To evaluate the anti-inflammatory efficacy of EEM containing active compounds, zebrafish embryos exposed to LPS were compared with and without EEM treatment for nitric oxide (NO) production, reactive oxygen species (ROS) generation, and cell death two days after fertilization. These evaluations indicate that EEM alleviated inflammation by inhibiting cell death, ROS, and NO generation induced by LPS treatment. According to these results, eckmaxol and dieckol isolated from brown seaweed E. maxima could be considered effective anti-inflammatory agents as pharmaceutical and functional food ingredients.     

拮抗茄子黄萎病菌土壤放线菌的分离筛选和鉴定

为了研究土壤拮抗性放线菌对茄子黄萎病的生防价值,从陕西省延安市宝塔区与汉中地区土壤中分离、纯化获得154株具有纤维素降解活性的放线菌。采用平板对峙培养法进行初筛,用生长速率法对抑菌效果较好的菌株ZX-10-4进行抑菌谱的测定,并进行盆栽试验验证其对茄子黄萎病的防治效果,通过形态、培养特征及16S rDNA序列分析研究其分类地位。结果显示,有17株放线菌对茄子黄萎病菌抑菌效果较好且抑菌活性稳定,其中菌株ZX-10-4的抑菌活性最高,抑菌率达90.15%;活性菌株ZX-10-4发酵原液对茄子黄萎病的保护和治疗作用分别为70.00%和60.33%,稀释5倍液的防效也在50%以上。根据形态、培养特征、生理生化指标和16S rDNA序列分析,初步确定菌株ZX-10-4为白网链霉菌Streptomyces albireticuli的近似种。     

小麦类菌原体兰矮病研究简报

 小麦类菌原体兰矮病(Wheat Mycoplasna like-oaganism Blue Dwarf-WMBD)是北方麦麦区的一种主要病害,特别是在间套麦田和麦草覆盖保墒的旱农栽培麦区发生严重,其是我国在小麦上新近发现的第一个类菌原体病害。     

法国野燕麦单粒种子DNA 快速提取方法的比较

本文以法国野燕麦作为材料,比较了CTAB、SDS、NaOH裂解3种方法提取单粒种子DNA的效果,根据核糖体DNA的PCR扩增结果,结合准确、快速和简便的原则,挑选出快速提取单粒种子DNA的方法,为杂草种子快速分子鉴定打下基础。     

苹果上的瘿蚊科害虫

苹果的果实和植株具有较高的经济价值,因而苹果上的害虫需要得到重视。本文为国内首次详细介绍苹果上的瘿蚊科害虫的形态特征、地理分布、生物学特性和为害特点,并编写世界已知4种苹果瘿蚊的雄性成虫形态特征检索表,以期使读者较全面地了解这类害虫的经济重要性,为进一步鉴定、检疫和防治这类瘿蚊科害虫提供参考。     

进境荷兰郁金香种苗中南芥菜花叶病毒的鉴定

为了防止南芥菜花叶病毒(Arabis mosaic virus,ArMV)传入我国,采用血清学、分子生物学、电镜观察及生物学接种等方法对从荷兰进口的郁金香种苗进行了ArMV检测。结果表明:ArMV血清学反应为强阳性;RT-PCR反应扩增出370bp的特异性目标条带;RT-PCR产物与已报道的3个ArMV部分外壳蛋白基因的核苷酸序列同源性为91.00%～93.24%,氨基酸序列同源性为99%～100%;免疫电镜观察到叶片病汁液中含有直径约30nm的球状病毒粒体;该病毒在黄花烟、白肋烟、矮牵牛和昆诺藜等鉴别寄主上引起坏死和褪绿等典型症状,经DAS-ELISA检测,这些接种寄主均呈ArMV血清学阳性反应。故将该病毒鉴定为南芥菜花叶病毒。