笃斯越桔硬枝扦插繁殖技术研究

采用笃斯越桔硬枝扦插,用生根剂处理插穗,并在不同的基质上进行扦插试验,结果表明:笃斯越桔硬枝扦插用河沙作为基质最好,生根剂ABT处理的插穗生根率最高。     

13～18周龄笼养蛋鸭适宜蛋白质和蛋氨酸水平的研究

用 32 4羽满 1 2周龄的金定鸭 ,通过饲养试验、代谢试验、屠宰试验和对试鸭产蛋期 (1 9～ 2 8周龄 )产蛋性能的观察 ,研究了在等能条件下(ME :1 1 51MJ/kg) ,不同蛋白质水平 (CP :1 3 %、 1 5 %、 1 7% )和蛋氨酸水平 (Met/CP :0 0 1 7、 0 0 2 2、 0 0 2 7)组成的 9种饲粮对 1 3～ 1 8周龄笼养蛋鸭的生长性能、养分利用、体成分沉积和产蛋期产蛋性能的影响。结果表明 :该阶段饲粮粗蛋白、蛋氨酸水平对体增重影响不显著 (P >0 0 5) ;体增重与蛋氨酸 (% )呈一定的正相关 (r=0 4574) ;CP为 1 3%、Met/CP为 0 0 2 7组采食量一直较稳定、较高 ,且饲料效率又好。代谢试验 :不同粗蛋白、蛋氨酸水平饲粮对试鸭氮存留率和能量的利用率影响显著(P <0 0 5或P<0 0 1 ) ,且它们随饲粮CP水平的升高而降低 ,随蛋氨酸水平升高而升高 ;磷的利用率也分别以CP为 1 3%的水平和Met/CP为 0 0 2 7的水平极显著地高于其他水平对磷利用率 (P <0 0 1 )。屠宰试验 :当Met/CP为 0 0 2 7时 ,体氮沉积量和体磷沉积量最佳。产蛋性能 :开产日龄和高峰日龄与 1 3～ 1 8周龄CP食入量呈弱的负相关 (r=- 0 1 83 3和r=- 0 0 984) ,与蛋氨酸食入量呈正相关 (r =0 5780和r =0 435 0 )。饲粮粗蛋白、蛋氨酸水平对 1 9～ 2 8周龄     

离心式压缩机喘振问题分析

当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行.     

我国物流行业上市公司资本结构影响因素实证研究

采用实证分析和规范分析相结合的方法来研究影响物流行业上市公司资本结构的内外因素，并提出相应的对策与建议，以期能为物流行业上市公司制定合理的资本结构提供实证数据和参考。     

陕甘宁老区脆弱生态环境定量评价——以榆林、延安两市为例

位于黄土高原中部的陕甘宁老区生态环境极为脆弱 ,近年来由于气候、人类开发资源等自然和人为原因 ,使生态环境的脆弱程度升高。选定年降水量、年均温、蒸发量等 8个指标 ,定量评价各县 1 970 - 2 0 0 0年的脆弱度状况 ,结果表明榆林、延安两市生态环境整体脆弱 ,脆弱度存在空间差异但差异不明显 ,时间段上的波动幅度不大。陕甘宁老区脆弱生态环境具有不稳定性 ,对外界干扰较敏感。     

Relationship between expression of somatostatin and DNA ploidy in breast cancer

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer.     

The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.     

The research development of endothelial progenitor cells

Endothelial progenitor cell (EPC) is a kind of directional cell that is able to differentiate to endothelial cell. The role of EPC is associated not only with vasculogenesis during embryonic development but also with physiological organ maintenance and angiogenesis during postnatal and adult period. There is a good clinical therapeutic prospective use for EPC in the treatment of ischemia diseases and inhibition of tumor angiogenesis.     

鸡抗球虫药的体外筛选综述

抗鸡球虫药的筛选分为体内筛选和体外筛选。体外筛选具有简易、迅速、准确和经济的优点。本文在前人大量工作的基础上，总结了体外筛选的全过程，并分述了细胞培养筛选法和鸡胚培养筛选法。     

铅胁迫对金丝草生长及生理生化的影响

采用土培方法,探究不同梯度铅胁迫(0、1000、2000和3000 mg·kg^-1)对金丝草生长形态、体内抗氧化系统和渗透调节物质的影响。结果表明:低浓度(1000 mg·kg^-1)处理会诱导金丝草叶片过氧化物酶(POD)活性、可溶性蛋白(SP)含量和根系抗超氧阴离子自由基活力(ASAFR)、可溶性糖(SS)及脯氨酸(Pro)含量增加,使得金丝草植株总抗氧化能力(T-AOC)处于较高水平,促进了金丝草株高、叶长和生物量的增加。随胁迫浓度增加,高浓度(2000~3000 mg·kg^-1)处理下,金丝草叶片和根系丙二醛(MDA)含量迅速增加,株高、叶长、叶面积和生物量下降,生长受到抑制。但金丝草通过增强叶片和根系POD、过氧化氢酶(CAT)活性来抵御过氧化作用,提高可溶性蛋白和可溶性糖含量维持细胞正常运作,增加根系生物量占比来加强根系发育,一定程度适应了高浓度铅胁迫。综上表明,金丝草主要通过叶片和根系不同抗氧化酶差异化响应、提高渗透调节物质含量,提升金丝草植株总抗氧化能力等途径来提高Pb耐性,对Pb污染矿区植物修复有较大潜力。