全文获取类型
收费全文 | 57篇 |
免费 | 3篇 |
专业分类
林业 | 2篇 |
农学 | 1篇 |
8篇 | |
综合类 | 14篇 |
农作物 | 10篇 |
畜牧兽医 | 17篇 |
园艺 | 4篇 |
植物保护 | 4篇 |
出版年
2021年 | 1篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2014年 | 1篇 |
2013年 | 2篇 |
2012年 | 1篇 |
2011年 | 2篇 |
2010年 | 1篇 |
2008年 | 2篇 |
2007年 | 2篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 1篇 |
2002年 | 3篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1968年 | 1篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1963年 | 1篇 |
1962年 | 3篇 |
1960年 | 1篇 |
1907年 | 1篇 |
1903年 | 1篇 |
1901年 | 2篇 |
1897年 | 1篇 |
排序方式: 共有60条查询结果,搜索用时 31 毫秒
1.
2.
3.
Winston I 《Science (New York, N.Y.)》1901,13(317):129-133
4.
5.
6.
Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread gene-silencing information between tissues but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi. 相似文献
7.
Naturally occurring antibodies reactive with sperm proteins: apparent deficiency in AIDS sera 总被引:3,自引:0,他引:3
T C Rodman J Laurence F H Pruslin N Chiorazzi R Winston 《Science (New York, N.Y.)》1985,228(4704):1211-1215
A set of naturally occurring immunoglobulin M (IgM) antibodies that are reactive with a defined subset of proteins in the acrosomal cap region of human sperm has been identified. These antibodies are present in a broad spectrum of human sera from males and females, 1 day to 40 years of age, and are absent or markedly deficient in a large proportion of sera from individuals with the acquired immune deficiency syndrome (AIDS) or at risk for AIDS. The subset of proteins with which the IgM antibodies are reactive includes a factor (or factors) capable of inhibiting lectin-induced T-lymphocyte proliferation. The prevalence of the sperm-reactive IgM antibodies indicates that they are not elicited by sperm. Further, immunoreactivity of the sperm proteins resulting in depletion of specific circulating IgM antibodies, or other interactions between the sperm proteins and elements of the immune system, may be a factor in the suppressed state of the immune system in AIDS. 相似文献
8.
Anderson WA Amasino RM Ares M Banerjee U Bartel B Corces VG Drennan CL Elgin SC Epstein IR Fanning E Guillette LJ Handelsman J Hatfull GF Hoy RR Kelley D Leinwand LA Losick R Lu Y Lynn DG Neuhauser C O'Dowd DK Olivera T Pevzner P Richards-Kortum RR Rine J Sah RL Strobel SA Walker GC Walt DR Warner IM Wessler S Willard HF Zare RN 《Science (New York, N.Y.)》2011,334(6057):760-761
9.
Thibodeau MS Poore MH Hagler WM Rogers GM 《Journal of agricultural and food chemistry》2004,52(2):380-384
Unfortunate bovine fatalities occurring after ingestion of mold-damaged sweetpotatoes preclude the use of the culled tubers in livestock feed. In cattle, mold-damaged sweetpotatoes induce an acute respiratory distress syndrome resulting in asphyxiation. Because of this potential toxicity and the general abundance of culled sweetpotatoes, the detoxification efficacy of ensiling was explored since it is an easy and economically viable technique often applied to preserve livestock feed. Sweetpotato slices with or without mold damage were stored either frozen (to represent unfermented samples) or fermented for 6 weeks at room temperature. Following fermentation, organic extracts were generated for administration to mice. Thirty hours following administration of the extracts, mice were evaluated for gross and microscopic lesions affecting the lungs, liver, and kidneys. Fermentation of 6 weeks duration was observed to inadequately eliminate the lung, liver, and kidney toxicity caused by mold-damaged sweetpotatoes. In fact, fermentation exacerbated the hepatotoxicity of mold-damaged sweetpotatoes. This is also the first demonstration that sweetpotato regions lacking visible mold damage can induce lung and kidney injury, which, however, is preventable by fermentation. 相似文献
10.
Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA) 总被引:2,自引:0,他引:2
S Winston S Fiscus L Hesterberg T Matsushita M Mildbrand J Porter Y Teramoto 《Veterinary immunology and immunopathology》1987,17(1-4):453-464
The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized. 相似文献