Root system architecture (RSA) determines unevenly distributed water and nutrient availability in soil. Genetic improvement of RSA, therefore, is related to crop production. However, RSA phenotyping has been carried out less frequently than above-ground phenotyping because measuring roots in the soil is difficult and labor intensive. Recent advancements have led to the digitalization of plant measurements; this digital phenotyping has been widely used for measurements of both above-ground and RSA traits. Digital phenotyping for RSA is slower and more difficult than for above-ground traits because the roots are hidden underground. In this review, we summarized recent trends in digital phenotyping for RSA traits. We classified the sample types into three categories: soil block containing roots, section of soil block, and root sample. Examples of the use of digital phenotyping are presented for each category. We also discussed room for improvement in digital phenotyping in each category. 相似文献
Invasive ecosystem engineers can facilitate their invasions by modifying the physical environment to improve their own performance, but this positive feedback process has rarely been tested empirically except in sessile organisms. The invasive crayfish Procambarus clarkii is an ecosystem engineer that destroys aquatic macrophytes, which provide a physical refuge for animal prey, and this destruction is likely to enhance vulnerability to predators. Using two series of mesocosm experiments, we tested the hypothesis that the invasive crayfish increases its feeding efficiency on animal prey by reducing submerged macrophytes, thus increasing its individual growth rate in a positive density-dependent manner.
Results
In the first experiment, increasing crayfish density reduced both macrophytes and animal prey (dragonfly and chironomid larvae) and, importantly, increased the growth rate of individual crayfish, in accordance with our expectation. In the second experiment, we used artificial macrophytes to clarify whether the physical architecture of macrophytes itself protects animal prey and limits crayfish growth rate. Increasing the artificial macrophyte quantity not only increased the survival of animal prey, but also retarded the crayfish growth rate.
Conclusions
We conclude that macrophytes strengthen bottom-up control of crayfish, but this effect can be relaxed by increasing the density of crayfish via reduction in macrophytes. This positive feedback process may explain the crayfish outbreaks and regime shifts occasionally observed in invaded freshwater ecosystems.
We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR. 相似文献
The spread of coronavirus disease 2019 (COVID-19) and subsequent lockdown measures have impacted economies and industries worldwide. The fisheries industry witnessed a sharp decline in demand and a slump in fish prices due to its dependence on the food service industry. It is important to quantitatively assess those fish species affected most and the extent of the pandemic’s impact on them, to take specific countermeasures. We propose a time-series analysis as an alternative to the current practice of using ad hoc year-on-year comparisons. Although the pandemic makes it difficult to construct a counterfactual approach due to the lack of an appropriate control group, we use time-series forecasting to simulate normal conditions using pre-pandemic data. In Tokyo, the unit price of fish species that were negatively impacted by the food services industry dropped by 12.65% to 14.64%, and by 26.08% to 28.22% after the declaration of a state of emergency. Seasonality, short weekly cycles, and short-term market trends are factors that affect the price of fish. Species-specific impact estimates related to the COVID-19 pandemic can allow policymakers to implement recovery measures in a more targeted and effective manner. The results of our analysis can increase fishers’ and policymakers’ awareness of the usefulness of economic analyses and incentivize them to release data to establish a system to accumulate and analyze data strategically for urgent and appropriate interventions in the fisheries industry.
The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized. 相似文献
Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay for the detection of canine parvovirus (CPV) antigen in fecal samples. The assay was specific for the hemagglutinating protein of CPV and detected as little as 1.5 ng of virus within a 15-minute incubation period. The use of monoclonal antibodies against 2 epitopes on the CPV antigen permitted the simultaneous addition of test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation. Clinical studies revealed greater than 95% correlation between enzyme-linked immunosorbent assay results and hemagglutination titers. 相似文献
Mother–infant bonding is a universal relationship of all mammalian species. Here, we describe the role of reciprocal communication between mother and infant in the formation of bonding for several mammalian species. Mother–infant bond formation is reinforced by various social cues or stimuli, including communicative signals, such as odor and vocalizations, or tactile stimuli. The mother also develops cross‐modal sensory recognition of the infant, during bond formation. Many studies have indicated that the oxytocin neural system plays a pivotal role in bond formation by the mother; however, the underlying neural mechanisms for infants have not yet been clarified. The comparative understanding of cognitive functions of mother and infants may help us understand the biological significance of mother–infant communication in mammalian species. 相似文献
To investigate the reproductive traits and diel activity ofMonochamus saltuarius, 14 pairs of the adults were reared and observed at each of 20°C and 25°C. Both sexes were more active at 25°C than at 20°C
irrespective of the presence or absence of light. A diel change in behavior during 24 h was found on 33% of a total of 48
observations of three age classes of both sexes. No significant difference was found in the mean longevity between sexes or
between the two temperature conditions. The time required for reproductive maturation showed no significant difference within
the same sex when reared at 20°C and 25°C. The mean oviposition period was significantly longer at 25°C (28.8 days) than at
20°C (12.2 days). The mean lifetime fecundity was significantly greater at 25°C than at 20°C. There was no significant difference
in the oviposition ratio (number of eggs deposited/number of oviposition wounds excavated), oviposition rate or hatchability
for females between the two temperature conditions. 相似文献