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1.
Huseyin Yilmaz Eda Altan Nuri Turan Aydin Gurel Damla Haktanir Kivilcim Sonmez Sezgin Deniz Ahmet Gulcubuk Emre Gur Gunes Sonmez Juergen A. Richt 《Journal of Equine Veterinary Science》2012
There has been an increase in outbreaks of neuropathogenic equine herpesvirus-1 (EHV-1) in the United States and Europe. However, the presence and frequency of neuropathogenic EHV-1 in Turkish horses are not known at present. This study aimed to investigate the frequency of EHV-1 and neuropathogenic strains of EHV-1 in the Marmara Region of Turkey. Samples were analyzed for the presence of EHV-1 and neuropathogenic EHV-1 by real-time PCR TaqMan probe assays. Overall detection rate of EHV-1 was 45.5% (51 of 112). The detection rates were 70.5% (24 of 34) in aborted fetuses, 53.3% (8 of 15) in neonatal deads, 66.6% (4 of 6) in foals, 40% (2 of 5) in dead mares, and 25% (13 of 52) in living mares. Overall detection rate of neuropathogenic EHV-1 was 7.8% (4 of 51), and the real-time PCR results were confirmed by sequencing. Neuropathogenic strains of EHV-1 were detected in the brain and lung of two mares with neurological disease but without a history of abortion, in the brain of a foal that died of respiratory disorder, and in the nasal swab from a mare with a history of abortion. On histopathology, nonpurulent meningoencephalitis, hemorrhages, and vasculitis were seen in the brain. In conclusion, results of this study indicated, for the first time, that the neuropathogenic EHV-1 is circulating in the Marmara Region of Turkey. The results of this study also show that the current risk for non-neuropathogenic strains is high, whereas risk for the neuropathogenic EHV-1-G2254 strain seems to be low. As outbreaks of EHV-1 continue in the Marmara region of Turkey, surveillance for neuropathogenic EHV-1 genotype should be maintained. 相似文献
2.
Sebahattin Albayrak Özgür Töngel İlknur Ayan Zeki Acar 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(2):154-161
Abstract This three-year study (2003–2005) aimed to improve the yield and quality of pastures growing naturally that are colonized by naturally occurring vegetation without agricultural input under hazelnut (Corylus sp.) orchards in the middle and eastern Black Sea regions of Turkey. There were eight treatments: 1) control; 2) fertilizer only (triple superphosphate 44% and calcium ammonium nitrate 26%) (80kg ha?1 P and 60 kg ha?1 N in Samsun; 100 kg ha?1 P and 80 kg ha?1 N in Ordu; 100 kg ha?1 P and 40 kg ha?1 N in Giresun); 3) lime only (calcium carbonate 94%) (3.0 t ha?1 lime in Samsun; 4.5 t ha?1 lime in Ordu and Giresun); 4) early cut only; 5) soil aeration only; 6) fertilizer+lime; 7) fertilizer+lime+early cut; 8) fertilizer+lime+soil aeration, laid out in a randomized complete block design with four replicates at each location. The highest dry matter (DM) yield of kg ha?1 and crude protein content (%) was obtained from the treatments that included fertilizer. There was no difference in DM production between any of the combination treatments that involved fertilizer and the fertilizer alone treatment. Only lime and aeration applications also increased yield compared to control, but not as much as did any treatment including fertilizer. Crude protein content of the pasture ranged from 13.3 to 18.1% across locations. Nitrogen and phosphorus fertilizer are recommended to improve DM yields and herbage quality for pastures under hazelnut orchards. 相似文献
3.
Emel Alkan Fatih Kapukıran Elif Öztürk Er Dotse Selali Chormey Seyfullah Keyf Nizamettin Özdoğan Sezgin Bakırdere 《Water, air, and soil pollution》2018,229(9):298
The potential risk of pesticides to cause harm to humans and other organisms even at trace levels calls for sensitive and accurate analytical techniques for their simultaneous qualitative and quantitative determinations. In this study, a sensitive binary dispersive liquid-liquid microextraction (B-DLLME) strategy was developed for the simultaneous determination of phorate and oxyfluorfen by gas chromatography mass spectrometry after extraction/preconcentration from aqueous solution. An experimental design was used to optimize parameters of the B-DLLME method to obtain maximum outcome. Under the optimum conditions of B-DLLME, the limit of detection (LOD) for phorate and oxyfluorfen were found to be 0.41 μg L?1 and 0.54 μg L?1, respectively. The detection limits correlate to about 37 and 73 folds enhancement in detection powers when compared to direct GC-MS determination of phorate and oxyfluorfen, respectively. In order to find out the applicability of developed method to real samples, recovery tests were performed for 20 μg L?1 of phorate and oxyfluorfen spiked in well water samples. Percent recovery values were found to be 94.5% for phorate and 101.9% for oxyfluorfen. 相似文献
4.
OBJECTIVE: To determine the clinical efficacy of subconjunctival injection of clindamycin in the treatment of naturally occurring infectious bovine keratoconjunctivitis (IBK). ANIMALS STUDIED: Clinically, out of 81 animals examined, 46 were found to be suffering from IBK of variable severity. The ocular secretions were collected and cultured for Moraxella bovis. The study included 36 Holstein cattle from which M. Bovis was isolated. These animals ranged between 4 and 28 months of age. PROCEDURES: The severity of the clinical findings were scored as normal, mild, moderate, and severe. Clindamycin was injected subconjunctivally at a total dose of 150 mg (1 mL), once daily for 3 days to the test group (n = 18); isotonic saline solution (1 mL) was administered to the control group. After treatment, all cattle were re-examined and clinical response was evaluated on days 3, 7 and 15 post-treatment. RESULTS: Compared with the control group and prior to treatment, all active lesions such as blepharospasm, epiphora, photophobia, chemosis, corneal edema, and corneal ulceration were generally resolved by day 15 after subconjunctival injection of clindamycin. Severity of IBK lesions increased on days 3 and 7, compared to baseline in the control group administered isotonic saline solution. CONCLUSIONS: The results of this study suggest that subconjunctival injection of clindamycin is effective in the treatment of naturally occurring infectious bovine keratoconjunctivitis. 相似文献
5.
This study presents the development of a sensitive and accurate dispersive liquid-liquid microextraction method using binary solvents for the simultaneous determination of endocrine disruptive compounds by GC-MS. Optimum binary solvent and dispersive solvent amounts were determined using an experimental design. The main effects of these parameters and their interaction effects were assessed using analysis of variance. The detection limits of the analytes under optimal experimental conditions ranged between 0.16–1.5 ng/mL. All analytes exhibited good linearity over broad calibration ranges, and high precision (%RSD?<?8.0%) was obtained for six repeated readings of the lowest concentrations of the calibration plots. The method’s applicability and accuracy were tested on two municipal wastewater samples spiked at 10, 50, and 100 ng/mL. The recovery results obtained ranged between 82 and 108%, indicating that the method can be used to quantify the analytes in wastewater matrix with substantial accuracy. In addition, matrix matching calibration method was used to improve the percent recovery (≈?100%) for a waste sludge sample spiked at 50 ng/mL. 相似文献
6.
Maral Selin Fındıkoğlu Merve Fırat Dotse Selali Chormey Fatma Turak Çiğdem Şahin Sezgin Bakırdere 《Water, air, and soil pollution》2018,229(2):37
This study presents a combination of dispersive liquid-liquid-solidified floating organic drop microextraction (DLLSFODM) and slotted quartz tube (SQT) with conventional flame atomic absorption spectrometry (FAAS) to improve the sensitivity for cadmium determination. A ligand namely 2-(4-methylphenyl)-1H-imidazo-[4,5-f]-[1,10]-phenanthroline which has not been used in trace analyte determination was used to form a cadmium complex. Stepwise optimization of parameters affecting complex formation (pH, ligand, and buffer solution) and extraction (extraction and dispersive solvents, salt effect and mixing) was done to maximize cadmium absorbance. The slotted quartz tube was fitted onto the flame burner and optimized to increase residence time of atoms in the flame. Instrumental parameters such as sample and fuel flow rate were also optimized to further enhance the absorbance signal for cadmium. Using optimal parameters and values, the limits of detection and quantification were determined to be 0.81 and 2.69 μg L?1, respectively. Low percent relative standard deviations (<?6.0%) indicated good precision for both extraction and instrumental measurements. Recovery tests were used to determine the accuracy of the method and the recovery results obtained were between 88 and 113%. 相似文献
7.
Polley Shamik De Sachinandan Brahma Biswajit Mukherjee Ayan P.V. Vinesh Batabyal Subhasis Arora Jaspreet Singh Pan Subhransu Samanta Ashis Kumar Datta Tirtha Kumar Goswami Surender Lal 《Tropical animal health and production》2010,42(5):985-993
Tropical Animal Health and Production - Mutation studies in different prolific sheep breeds have shown that the transforming growth factor beta super family ligands viz. the growth differentiation... 相似文献
8.
Abhijit Majumdar Anindya Ghosh Shib Sankar Saha Ayan Roy Subir Barman Dhrubajyoti Panigrahi Anjan Biswas 《Fibers and Polymers》2008,9(2):240-245
Aesthetic properties of fabrics have been considered as the most important fabric attribute for years. However, recently there
has been a paradigm shift in the domain of textile material applications and consequently more emphasis is now being given
on the mechanical and functional properties of fabrics rather than its aesthetic appeal. Moreover, in certain woven fabrics
used for technical applications, strength is a decisive quality parameter. In this work, tensile strength of plain woven fabrics
has been predicted by using two empirical modelling methods namely artificial neural network (ANN) and linear regression.
Warp yarn strength, warp yarn elongation, ends per inch (EPI), picks per inch (PPI) and weft count (Ne) were used as input
parameters. Both the models were able to predict the fabric strength with reasonably good precision although ANN model demonstrated
higher prediction accuracy and generalization ability than the regression model. The warp yarn strength and EPI were found
to be the two most significant factors influencing fabric strength in warp direction. 相似文献
9.
Observations were made on six grafts for each of 25 clones in three Scots pine (Pinus sylvestris) seed orchards in Turkey. The characters studied were number of female and male strobili, height below and above the longest
branch, total height; diameter at base and breast height, crown diameter, and number of branches. Variation, broad-sense heritability
(H
2) and correlations between characters were estimated.
Variation among clones was lower than among grafts within clone for all characters. The genetic variation for number of strobili
varied between 0 and 17% of total variation, while that for growth characters values varied between 2 and 13%. The number
of female strobili appeared more variable among trees than the number of male strobili. H
2 was not consistently high for any character or seed orchard. The number of strobili increased with the size of the tree,
but not dramatically. Correlations between measures of tree size (both on clone level and individual graft level) and the
number of strobili were in the magnitude r ≈ 0.3. Diameter at breast height seems a reasonable predictor for number of strobili. 相似文献
10.