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排序方式: 共有227条查询结果,搜索用时 15 毫秒
1.
Kamonpan Awaiwanont Wisnu Gunarso Munefumi Sameshima Seiichi Hayashi Gunzo Kawamura 《Fisheries Science》2001,67(5):804-810
ABSTRACT: Morphology of the photoreceptor cells and tapetum of the Japanese anchovy Engraulis japonicus was studied by histologically and by chemical analysis. The Japanese anchovy has a duplex retina. The cones form parallel rows consisting of alternately placed long cones and bifid cones. Both types of cones are intimately associated and form triple units that are regularly spaced along the row of cones. The rods are grouped and stacked. This fish has a retinal tapetum lucidum composed of guanine and hypoxanthine. Three structures of the tapetum lucidum were recognized: platelet, diamond and rod types. Photomechanical changes include movements of the photoreceptor cells and the retinal tapetum. The retina of the Japanese anchovy is thought to be highly sensitive and well adapted to a dim light environment. 相似文献
2.
Competitive enzyme-linked immunosorbent assay for the detection of the antibodies specific to akabane virus. 总被引:5,自引:0,他引:5
Tomoyuki Tsuda Kazuo Yoshida Tohru Yanase Seiichi Ohashi Makoto Yamakawa 《Journal of veterinary diagnostic investigation》2004,16(6):571-576
A competitive enzyme-linked immunosorbent assay (C-ELISA) using neutralizing monoclonal antibodies (MAbs) against Akabane virus (AKAV) was developed to detect antibodies to AKAV in cattle sera. The performance of the test using 7 different competitor MAbs was evaluated in sequential serum samples and sera from cattle infected with various bovine arboviruses. The dynamics of the antibody response expressed by percentage of inhibition (PI) in C-ELISA coincided with those of neutralizing antibody titers in sequential serum samples from 2 cattle experimentally infected with AKAV. The value of PI in C-ELISA for convalescent sera from cattle infected with arboviruses correlated with the neutralizing antibody titer to AKAV but was unaffected by the antibodies to other arboviruses. In the validation experiment of C-ELISA using 286 bovine sera previously examined for the AKAV antibody by serum neutralization (SN) test, the relative specificity of C-ELISA was more than 98%, whereas the relative sensitivities of individual MAbs ranged from 49% to 82.2%. Overall agreement between C-ELISA and the SN test varied from 72% to 90% depending on the MAb. These results suggest that the C-ELISA is acceptable as a rapid and specific method for detecting antibodies to AKAV and is a potential alternative to the SN test. 相似文献
3.
C. Ye S. Fukai I. D. Godwin H. Koh R. Reinke Y. Zhou C. Lambrides W. Jiang P. Snell E. Redoña 《Euphytica》2010,176(3):291-301
Low temperature is a major abiotic stress for rice cultivation, causing serious yield loss in many countries. To identify
QTL controlling low temperature induced spikelet sterility in rice, the progeny of F2, BC1F1 and BC2F1 populations derived
from a Reiziq × Lijiangheigu cross were exposed to 21/15°C for 15 days at the booting stage, and spikelet sterility was assessed.
For genotyping, 92 polymorphic markers from 373 SSR and 325 STS primer pairs were used. A major QTL was initially indentified
on the short arm of chromosome 10 by selective genotyping using highly tolerant and susceptible progeny from F2 and BC1F1
populations. The QTL (qLTSPKST10.1) was validated and mapped by genotyping the entire F2 (282 progeny) and BC1F1 (84 progeny)
populations. The results from the F2 population showed that qLTSPKST10.1 could explain 20.5% of the variation in spikelet
sterility caused by low temperature treatment with additive (a = 14.4) and dominant effect (d = −7.5). From the analysis of
98 selected BC2F1 progeny, the QTL located in the 3.5 cM interval between S10010.9 and S10014.4 was further confirmed. Based
on the studies of 3 generations in 2 years, it was clear that the QTL on chromosome 10 is a major determinant of the control
of low temperature induced spikelet sterility at booting stage. 相似文献
4.
To elucidate the formation mechanism of acidsoluble lignin (ASL) formed in the Klason lignin determination, beech wood meals were treated with sulfuric acid (SA) under various conditions, and the ASL solution was extracted with CHC13. The results indicated the following: (1) wood components yielding ASL are dissolved in 72% SA during the initial stage; (2) the quantity of ASL is highest during the initial stage, then decreases with prolonged time of 72% SA treatment and finally reaches a constant value; (3) soluble lignin prepared by 72% SA treatment and subsequent standing in 3% SA again yield insoluble Klason lignin and ASL after boiling in 3% SA; and (4) about half the amount of ASL is dissolved in CHC13. The foregoing suggest that wood components yielding ASL are dissolved in 72% SA at the beginning and finally change to ASL after being subjected to depolymerization, hydrolysis, and other reactions. ASL may thus be composed of low-molecular-weight degradation products and hydrophilic derivatives of lignin. 相似文献
5.
Application of cationic polymer prepared from sulfuric acid lignin as a retention aid for usual rosin sizes to neutral papermaking 总被引:3,自引:0,他引:3
Yasuyuki?MatsushitaEmail author Ayuko?Iwatsuki Seiichi?Yasuda 《Journal of Wood Science》2004,50(6):540-544
Cationic polymers that acted as the retention aids for usual rosin sizes in neutral papermaking were prepared from sulfuric acid lignin (SAL), one type of acid lignin. To convert SAL to the cationic polymer (MP-SAL), SAL was phenolated and then treated by the Mannich reaction to introduce the amino groups. In the MP-SAL single system, MP-SAL exhibited high sizing effectiveness in neutral papermaking with the rosin emulsion size. However, MP-SAL showed no sizing effectiveness when soap rosin size was used. MP-SAL showed increased sizing effectiveness as the pK
a of the introduced amino group increased. From this and comparison of the sizing degrees of MP-SAL and polyethylenimine, which possesses a linear structure, it was suggested that the sizing effectiveness was not only affected by the charge density and molecular weight, but also by the basicity of the introduced amino groups and the molecular structure of the retention aid. In the alum–MP-SAL dual system, alum and MP-SAL synergistically enhanced the sizing effectiveness in the rosin emulsion sizing at neutral pH. In turn, this allowed a decrease in the MP-SAL dosage and resulted in a small decrease in brightness.Part of this report was presented at the 48th Lignin Symposium, October 2003, Fukui 相似文献
6.
Shun HIRASAWA Miki SHIMIZU Yuumi MARUI Miori KISHIMOTO Seiichi OKUNO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1603-1607
We designed a new method of
measuring the length of the ulnar nerve and determining standard values for F-wave
parameters of the ulnar nerve in clinically normal beagles. Nerve length must be precisely
measured to determine F-wave latency and conduction velocity. The length of the forelimb
has served as the length of the ulnar nerve for F-wave assessments, but report indicates
that F-wave latency is proportional to the length of the pathway traveled by nerve
impulses. Therefore, we measured the surface distance from a stimulus point to the spinous
process of the first thoracic vertebra (nerve length 1) and the anterior horn of the
scapula (nerve length 2) as landmarks through the olecranon and the shoulder blade
acromion. The correlation coefficients between the shortest F-wave latency and the length
of nerves 1, 2 or the forelimb were 0.61, 0.7 and 0.58. Nerve length 2 generated the
highest value. Furthermore, the anterior horn of the scapula was easily palpated in any
dog regardless of well-fed body. We concluded that nerve length 2 was optimal for
measuring the length of the ulnar nerve. 相似文献
7.
8.
9.
Hitoshi Kubota Takahide Doi Shoichiro Yamamoto Seiichi Watanabe 《Fisheries Science》2007,73(2):270-284
ABSTRACT: Stocking of exogenous, hatchery-reared white-spotted charr Salvelinus leucomaenis has been conducted throughout much of their range in Honshu Island, Japan, to increase angling opportunities. Although the native charr populations are thought to have declined because of hybridization with introduced fish, their distribution and genetic status have been uncertain. Fine population structures of charr in the upper Tone River drainage were examined using mitochondrial DNA and microsatellite analyses so as to clarify the presence of native populations. One common mtDNA haplotype was detected in all populations in the Ohashi River and Watarase River, and four and one tributary populations were monomorphic for such haplotypes, respectively. However, several haplotypes, considered to have originated from stocked hatchery fish, were observed in the stocked and the remaining populations. Judging from the genetic integrity over a fine geographic scale, the former were considered as indicative of native populations and the latter as admixtures with hatchery fish. Comparisons of genetic diversity, deviations from the Hardy–Weinberg equilibrium, principal component analysis, and relatedness estimations based on microsatellite DNA can also provide evidence for distinguishing native populations from those influenced by hatchery fish. 相似文献
10.