群井开采对毛乌素沙地地下水位的影响——以榆林市榆阳区昌汗敖包村土地开发项目为例

为探究毛乌素沙地大规模现代化高标准农田地下水开采对地下水位的影响，以榆林市榆阳区昌汗敖包村土地开发项目地下水监测网覆盖区作为研究区，选取分布于研究区的8眼机井进行了为期1年的地下水位埋深动态特征监测。结果表明:在灌溉期间，地下水位埋深呈先减小后增大的趋势；灌溉结束至进入下个灌溉周期之前，地下水位埋深逐渐增大至正常值。因此群井开采并未对项目区地下水位造成实质影响。      

氮吸附法分析生物质焦孔隙结构

采用氮吸附法对4种生物质焦(稻壳、树叶、玉米秆、棉花秆)的孔隙结构进行测量,结果表明,不同种类焦样的比表面积和孔径分布有明显差别,树叶的比表面积最大,为242.21 m2·g-1,玉米秆的比表面积最小,为0.81 m2·g-1.850℃时,稻壳、树叶、玉米秆焦样的孔径分布曲线在微孔和中孔范围各有一个分布峰,而棉花秆焦样的孔径分布曲线只在中孔范围内出现一个分布峰.热解温度是影响孔隙结构的一个重要因素,在高温条件下,同步热解得到的焦样的比表面积较大,微孔较多.在本研究中,600℃、850℃的稻壳焦样和850℃的树叶焦样具有较大的比表面积,比较适合做吸附剂.     

鸭大肠杆菌病流行病学与病原特性研究

对浙江等地鸭大肠杆菌病流行病学进行调查，并研究其发病特点，分离鉴定出致病性大肠杆菌26株；已对其中20株菌株进行血清型鉴定，主要为078，占70%（14/20），其次是O1，占15%（3/20）；人工感染试验表明，80%（8/10）菌株毒力强，皮下、肌肉、足蹼3种途径均易复制该病；病理组织学检查肝、肺、脾等，表现急性炎症，出血等病变；药敏试验表明，不同菌株对药物敏感性差异较大，除对鸭菌消、卡那霉素等高敏外，对多数常用抗菌药已耐药，最后就该病的防治提出了对策。     

烟草黑胫病菌(Phytophthora parasitica var. nicotianae)生理小种鉴定技术研究

在1980-1986年,对烟草黑胫病菌生理小种鉴定技术和方法进行了研究和探讨。结果表明,Nicotiana ncsophil(?),Burley21;Burley37;N.stocktonii是理想的鉴别寄主:整株接种方法优于其他的接种方法;接种物浓度以控制在相同的水平(10~3 ml)为宜。为减小生物测定的差异,所有菌系应一次完成测定。     

知识管理在医院图书馆人力资源管理中的应用

 

根据知识管理的概念与内涵，从以人为本的角度，探讨了医院图书馆人力资源的特点以及实

施知识管理的具体做法。

 

 

     

草莓成熟过程中NAD激酶、NADP磷酸酶活性

 研究了‘春星’草莓果实成熟时NAD 激酶、NADP 磷酸酶活性及NAD 激酶部分特性。结果表明, NAD 激酶和NADP 磷酸酶活性在果实绿色期到乳白期升高, 粉红期下降, 全红期又再次升高。NAD 激酶可被Ca²⁺ 强烈抑制, 被EGTA (Ca²⁺专一性螯合剂) 激活。随着成熟进程, NAD 激酶对ATP 的依赖性增加。     

Expression of alpha-smooth muscle actin in scar tissue and its relationship with apoptosis

AIM: To examine the expression of alpha-smooth muscle actin in scar tissue, and observe the phenomenon of apoptosis and its involvement in the process of pathological scarring and the presence of myofibroblasts or absence of cell in the dermis. To investigate the potential role of reparative cell apoptosis in hyperplastic scar formation. METHODS: The samples of scar were obtained from post-burn patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. TUNEL assays were performed to evaluate the number of apoptotic cells in scar versus normal skin. In situ hybridization and immunohistochemistry staining technique were employed to determine the expression of different dermis cells markers in scar tissue and normal skin. RESULTS: There existed evident difference in apoptotic cells in the dermis between scars tissue and normal human skin. The expression positive cells were much more in hyperplastic scars than that in normal human skin; the apoptotic cells of proliferative stage were slight more than that of mature stage. However, in proliferative stage, the number of apoptotic cells was higher for the combination of hyperplastic scar than normally healed flat scars. But in mature stage, no obviously difference was detected between hyperplastic scar and normally healed flat scar. The monoclonal anti-α smooth muscle actin (ASMA) expression was significantly stronger in proliferative stage than that of mature stage. CONCLUSIONS: With reconstitution of dermal tissue, myofibroblasts containing alpha-SM actin disappear under normal wound healing, probably as a result of apoptosis. The myofibroblast play a critical role in wound closure and in the pathologic sequelae of healing.     

外源乙烯对草莓果实微粒体Ca2+- ATPase活性和膜脂过氧化的调节

 以乳白期‘春星’草莓(Fragaria ananassa Duch．‘Chunxing’)果实为试材，研究了经钙调素(CaM)拮抗剂三氟拉嗪(TFP)、氯丙嗪(CPZ)各100 µmol·L^-1 和钙通道阻塞剂异博定(Verapamil)100 µmol·L^-1 预处理后再用乙烯(50 µL·L^-1 )处理的草莓果实中微粒体“Ca²⁺-ATP酶活性、O₂ 产生速率和MDA含量的变化。结果表明，外源乙烯对微粒体膜O₂ 产生速率无显著影响，处理早期提高微粒体膜Ca²⁺- ATPase总活性，对MDA含量影响不大；后期加速微粒体膜Ca²⁺- ATPase总活性下降，但仍保持较高的MDA含量和线粒体膜Ca²⁺-ATPase活性。CPZ、TFP和Verapamil预处理降低了上述乙烯处理下Ca²⁺- ATPase活性和MDA含量，但对微粒体膜O 产生速率亦无显著影响，这说明细胞内Ca²⁺-和CaM 可能参与了乙烯诱导的膜Ca²⁺-ATP酶活性与膜脂过氧化水平的调节。     

Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.     

鸭疫里氏杆菌形态特征的电镜观察

自鸭传染性浆膜炎的病死鸭中分离鉴定的鸭疫里氏杆菌(RA)Ⅰ、Ⅱ型两个菌株，用负染法和超薄切片法制备样品，于透射电镜下进行观察。结果观察到约占1／3的RA具有特殊的形态结构：在细菌的顶端或侧面有1～2个与菌体相连的芽状赘生物，有的也能从菌体上脱落下来。它在超薄切片中的大小约为菌体的1／3～1／5，能观察到其内部也具有类似菌体内部的结构。且具有类似菌体的“细胞壁”，类似于核体部分的结构位于“细胞壁”的一侧。而用相同的方法制作的鸭大肠杆菌(E．cozi)对照样品，却见不到这种特殊的形态结构。说明这种形态特征可能是RA特有的。