The x-ray structure of chicken skeletal muscle troponin C (TnC), the Ca2+-binding subunit of the troponin complex, shows that the protein is about 70 angstroms long with an unusual dumbbell shape. The carboxyl and amino domains are separated by a single long alpha helix of about nine turns. Only the two high-affinity Ca2+-Mg2+ sites of the COOH-domain are occupied by metal ions resulting in conformational differences between the COOH- and NH2-domains. These differences are probably important in the triggering of muscle contraction by TnC. Also the structure of TnC is relevant in understanding the function of other calcium-regulated proteins, in particular that of calmodulin because of its strong similarity in amino acid sequence. 相似文献
1. Wheat, triticale 131 or triticale 72‐S could replace up to half the maize in broiler diets without affecting performance. Replacing more of the maize on either an equal nitrogen basis or a weight basis adversely affected performance.
2. Triticale 72‐S appeared to be superior to triticale 131 as a direct replacement for maize, but both grains were inferior to maize as energy sources. 相似文献
1. Dry rabbit excreta were found to contain 188.1 g crude protein, 6.6 g non‐protein nitrogen, 0.3 g ammonia, 89.8 g moisture, 8.0 g ether extractives, 135.2 g crude fibre, 266.9 g ash and 19.18 MJ gross energy/kg. Methionine and lysine contents were 3.95 and 4.29 g/100 g protein, respectively.
2. In one experiment, 0, 50, 100 or 200 g rabbit excreta/kg were substituted for maize in a broiler diet. Chickens receiving the diet containing 200 g rabbit excreta/kg gained significantly less weight to 8 weeks of age than those receiving other diets.
3. In a second experiment, 0, 100, 150 or 200 g rabbit excreta/kg were substituted primarily for maize in a broiler diet to provide equal energy and protein contents. Weight gains to 8 weeks of birds receiving these diets did not differ significantly. The uncorrected metabolisable energy content of the excreta was 9.15 MJ/kg.
4. There were no gross pathological changes in the birds fed on the experimental diets. 相似文献
1. The nitrogen‐corrected metabolisable energy contents of the triticale varieties Multi Graze, Graze Grain 70, 204 and 131 were 12.28, 12.69, 12.37 and 12.03 MJ/kg respectively.
2. Increasing proportions (0, 25, 50 or 75%) of the maize of a diet were replaced by triticale 131 to provide diets of equal energy and nitrogen contents for broilers. These diets resulted in weight gains of 1.775, 1.782, 1.713 and 1.808 kg respectively at 8 weeks and there were no significant differences among efficiencies of food conversions.
3. Dressed carcass weights as percentages of body weight were 74.38, 75.01, 73.98 and 74.50 respectively and did not differ significantly.
4. Triticale in the diet did not seem to influence the relative weights of either liver or gizzard.
5. The flavour of the meat seemed to become less acceptable as the triticale in the diet increased but there were no significant differences in tenderness and juiciness of the meat caused by the treatments. 相似文献
Recent studies have demonstrated a strong relationship between the intestinal microbiota and the host health. As such, consumers are increasingly becoming more concerned about the potential effect of certain foods/feeds, particularly of transgenic origin on the gut microbiota. Although the European Food Safety Authority has recommended in their guidelines, to study the effect of transgenic food/feed on host-microbiota, yet, few studies have focused on the evaluation of such effects mainly due to culturing difficulties. Therefore, this study was intended to evaluate the potential adverse effects of transgenic diet consumption on some specific gut microflora (Lactobacillus group, Bifidobacterium genus, Escherichia coli subgroup and Enterococcus genus) of rabbits. A total of forty-eight rabbits were randomly assigned into four groups and fed a diet containing a variable proportion of transgenic cottonseeds at 0, 20, 30 and 40% inclusion level, respectively. Changes in the specific or total faecal bacterial population were monitored at five different experimental stages (i.e. 0, 45, 90, 135 and 180 days) using both the traditional plate count method (TM) and quantitative real-time PCR (qPCR). No significant differences (p > .05) were observed concerning numbers of specific bacteria or total bacteria between the control and experimental groups, though qPCR showed numerically higher values in terms of 16S rRNA gene copies as compared to the values obtained from TM. However, such numerical differences were biologically insignificant (p > .05). Similarly, no significant variations were noticed in the calculated B/E (log10 copies of Bifidobacterium per g faces/log10 copies of E. coli genome per g faeces) ratios in all the groups. All the ratios were in the range of 1.24 to 1.30 throughout the experiment, indicating a good balance of intestinal microflora and greater resistance to intestinal disorders. It is therefore concluded that feeding transgenic cottonseeds could not adversely affect the gut microflora of rabbits during a long-term study. 相似文献
The problem of real-time irrigation scheduling under limited water supply is considered. The goal is to develop an irrigation operation policy which maximizes crop yields and is responsive to current season changes in weather and other variables. Because irrigation decisions are sequential and dependent on crop and soil water status, and also because crop yields can only be known at the end of the season, the decisions are arrived at by a two-stage process. In the first or the design stage, irrigations are planned for the entire season at weekly intervals using historical data and an optimal irrigation scheduling model. In the second stage, the decisions for the subsequent weeks are revised each week after updating the status of the system with real time data up to that week and solving the irrigation optimization model once again for the new conditions. Thus, each week an irrigation decision is made, the entire planning horizon is kept in view. The procedure is illustrated by application to a case study. 相似文献