Physicochemical and Pharmaceutical Properties of Carboxymethyl Rice Starches Modified from Native Starches with Different Amylose Content

Carboxymethyl rice starches (CMRS) were prepared from nine strains of native rice starches with amylose contents of 14.7–29.1%. The reaction was conducted at 50°C for 120 min using monochloroacetic acid as a reagent under alkaline conditions and 1-propanol as a solvent. After determining the degree of substitution (DS), the physicochemical properties including water solubility, pH, and viscosity of 1% (w/v) solution, scanning electron microscopy (SEM), and X-ray diffraction (XRD) analyses of the granules, as well as some pharmaceutical properties of CMRS powders and pastes were investigated. The DS range was 0.25–0.40. All CMRS dissolved in unheated water and formed viscous gel. A good positive correlation was observed between amylose content and DS (r = 0.9278) but not viscosity. SEM and XRD concurrently revealed significant physical alteration of CMRS granules compared with those of native starches, which reflected the changes in the properties of CMRS. At 3% (w/w), CMRS can function as tablet binder in the wet granulation of both water-soluble and water-insoluble diluents. The tablets compressed from these granules showed good hardness with fewer capping problems compared with those prepared using the pregelatinized native rice starch as a binder. In addition, most CMRS pastes formed clear films with varying film characteristics, depending upon the amylose content of the native starches. This type of modified rice starch can potentially be employed as a tablet binder and film-former for pharmaceutical dosage formulations.     

Environmental conditions during root development: Drought constraint on cassava starch quality

Cassava has the reputation of being a drought tolerant crop, however, when deprived of water, plant and root development are affected. This ultimately will translate to an altered starch synthesis expressed by variation of starch quality. The magnitude of which, is influenced by the severity of stress conditions and stage of plant maturity. During early plant development,water stress retards growth, which will only be resumed after the immature plant has received sufficient water. Despite of an increased starch yield, the effect of initial water stress on starch quality is still sustained. In mature plants, starch quality is affected by environmental conditions prior to root harvest, especially the onset of rain after a stress period as indicated by a reduced starch paste. This paper presents the argument for extending the scope of traditional breeding programs beyond selecting for plant growth under drought conditions, to strategies that place greater emphasis on stabilizing starch quality. This revised version was published online in July 2006 with corrections to the Cover Date.     

Border crossing of Muslim women in southern‐border provinces of Thailand

In general, the main roles of married Thai Muslim women were as home makers good wives and good mothers. Nevertheless, both married and single women from rural areas have been increasingly obliged to work outside the household, locally and in other countries. People in rural areas are now faced with the difficulty of maintaining their livelihoods if they depended on agricultural production alone. In some instances, female migration might be a response to families not being able to survive on the incomes earned by the male household heads. In response, women in southern Thailand provinces use long‐standing social networks that facilitate their migration for work, because they benefit from the close proximity, language, and religion that they share with the destination area. Commonly, they travel to work in Malaysia by using a border pass, while some travel and work without any documents. The effects of crossing national borders on migrants themselves and on their communities are mixed, generally positive from an economic perspective, but negative from a social viewpoint. Socially negative responses reflect a system of social control in the region based on patriarchy.     

Prevalence of autoantibodies that bind to kidney tissues in cats and association risk with antibodies to feline viral rhinotracheitis,calicivirus, and panleukopenia

BackgroundThe feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP) vaccine, prepared from viruses grown in the Crandell-Rees feline kidney cell line, can induce antibodies to cross-react with feline kidney tissues.ObjectivesThis study surveyed the prevalence of autoantibodies to feline kidney tissues and their association with the frequency of FVRCP vaccination.MethodsSerum samples and kidneys were collected from 156 live and 26 cadaveric cats. Antibodies that bind to kidney tissues and antibodies to the FVRCP antigen were determined by enzyme-linked immunosorbent assay (ELISA), and kidney-bound antibody patterns were investigated by examining immunofluorescence. Proteins recognized by antibodies were identified by Western blot analysis.ResultsThe prevalences of autoantibodies that bind to kidney tissues in cats were 41% and 13% by ELISA and immunofluorescence, respectively. Kidney-bound antibodies were observed at interstitial cells, apical border, and cytoplasm of proximal and distal tubules; the antibodies were bound to proteins with molecular weights of 40, 47, 38, and 20 kDa. There was no direct link between vaccination and anti-kidney antibodies, but positive antibodies to kidney tissues were significantly associated with the anti-FVRCP antibody. The odds ratio or association in finding the autoantibody in cats with the antibody to FVRCP was 2.8 times higher than that in cats without the antibody to FVRCP.ConclusionsThese preliminary results demonstrate an association between anti-FVRCP and anti-cat kidney tissues. However, an increase in the risk of inducing kidney-bound antibodies by repeat vaccinations could not be shown directly. It will be interesting to expand the sample size and follow-up on whether these autoantibodies can lead to kidney function impairment.