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1.
Mario Chiari Mariagrazia Zanoni Silvia Tagliabue Antonio Lavazza Loris G Alborali 《Acta veterinaria Scandinavica》2013,55(1):42
Background
Salmonella species (spp.) are zoonotic enteric bacteria able to infect humans, livestock and wildlife.However, little is known about the prevalence and the presence of the different serovars in wildlife. Considering the wide distribution of wild boars and the feeding behaviour (omnivorous scavengers), wild boars may be a good indicator for environmental presence of Salmonella spp. The aims of this study were to determine the presence of Salmonella spp. in hunted wild boars and to determine the serotype the isolated strains.Findings
Over three hunting seasons, the intestinal contents of 1,313 boars hunted in northern Italy were sampled and cultured. Salmonella spp. were isolated from 326 boars (24.82%). Thirty different serovars belonging to three different S. enterica spp. were found. Twenty-one serovars of S. enterica subsp. Enterica were found including the human pathogens S. Typhimurium and S. Enteritidis. In addition, nine serovars belonging to S.enterica subsp. diarizonae and S. enterica subsp. houtenae were detected.Conclusions
Considering the widespread occurrence of wild boars in Europe, the epidemiological role of this species in relation to salmonellosis might be relevant and should be further investigated. Wild boars may act as healthy carriers of a wide range of Salmonella serotypes. 相似文献2.
de Lira GA de Andrade LM Florêncio KC da Silva MS Barbosa-Filho JM Leitão da-Cunha EV 《Fitoterapia》2002,73(4):356-358
The roots of Cissampelos sympodialis yielded Roraimine (1), a novel bisbenzylisoquinoline alkaloid and liriodenine (2), a known oxoaporphine alkaloid. 相似文献
3.
Melissa de Oliveira Santos-Garcia Rosângela Maria Simeão Resende Lucimara Chiari Maria Imaculada Zucchi Anete Pereira de Souza 《Euphytica》2011,178(2):185-193
Stylosanthes capitata and S. guianensis are important forage legumes for tropical areas. The only available estimates of S. capitata and S. guianensis outcrossing rates were based on morphological markers, and the genus is considered as being mainly self-pollinated. Here
we describe an estimation of the outcrossing rate in S. capitata and S. guianensis using microsatellite markers. The outcrossing rates were estimated in S. capitata and S. guianensis open-pollinated populations of 20 progenies consisting of ten individuals each. The multi locus outcrossing rate for S. capitata was estimated using 10 polymorphic loci, whereas five microsatellites were used for S. guianensis. The multi locus outcrossing rates for S. capitata and S. guianensis were 31 and 26%, respectively, suggesting a mixed mating system with predominance of autogamy. Comparison of single locus
and multi locus estimates of outcrossing rates indicated that little inbreeding other than selfing occurred. The estimated
Wright’s fixation index of the parental generation was lower than expected based on the multi locus outcrossing rate, possibly
resulting from the use of some heterozygous breeding genotypes for the study. The data on the outcrossing rate described here
are potentially useful for breeding programs and for maintenance of germplasm collections of these Stylosanthes species. 相似文献
4.
Guedes PM Veloso VM Gollob KJ Afonso LC Caldas IS Vianna P de Lana M Chiari E Bahia MT Galvão LM 《Veterinary immunology and immunopathology》2008,124(1-2):163-168
A systematic study following infection by various strains of the protozoan parasite, Trypanosoma cruzi, and the simultaneous monitoring of the humoral immune response together with the elicited cellular response, could add greatly to our understanding of differences between strains of this important human pathogen. In that sense, acute and chronic infections with distinct T. cruzi strains (Y, Berenice-78 and ABC) in Beagle dogs were studied through a longitudinal evaluation of immunoglobulin G (IgG), IgG1 and IgG2 isotypes (by ELISA and flow cytometry (FC)), as well as measurements of peripheral blood mononuclear cell (PBMC) proliferation over a 100-week period, and their correlation with cardiomegaly. Our results show that infected animals presenting cardiomegaly showed lower or absent levels of IgG1 during the chronic phase of the infection, when compared to those that did not show an increase in heart weight. In that manner, our results suggest that IgG1 could be used as a marker for cardiac pathogenicity in Chagas disease. 相似文献
5.
Summary Transgenic potato plants of cv. Désirée carrying an antibacterial gene, coding for a cecropin lytic peptide analogue, were
inoculated with a virulent strain ofPseudomonas solanacearum under controlled conditions. The disease index scored during three repeated infection trials indicated an increased variability
in plant response among the transgenic lines which gave either a more susceptible or a more resistant response to the pathogen
when compared with untransformed Désirée. Immunity toP. solanacearum was not observed, but it was possible to select a group of transgenic lines that showed resistance levels and disease development
curves comparable to the field resistant cv. Cruza 148. 相似文献
6.
Mario Chiari Nicola Ferrari Daniele Giardiello Paolo Lanfranchi Mariagrazia Zanoni Antonio Lavazza Loris G Alborali 《Acta veterinaria Scandinavica》2014,56(1)
Background
Salmonella spp. have been isolated from a wide range of wild animals. Opportunistic wild carnivores such as red foxes (Vulpes vulpes) and badgers (Meles meles) may act as environmental indicators or as potential sources of salmonellosis in humans. The present study characterizes Salmonella spp. isolated from the intestinal contents of hunted or dead red foxes (n = 509) and badgers (n = 17) in northern Italy.Findings
Thirty-one strains of Salmonella belonging to 3 Salmonella enterica subspecies were isolated. Fourteen different serovars of S. enterica subsp. enterica were identified, among which were serovars often associated with human illness.Conclusions
Wild opportunistic predators can influence the probability of infection of both domestic animals and humans through active shedding of the pathogen to the environment. The epidemiological role of wild carnivores in the spread of salmonellosis needs to be further studied. 相似文献7.
8.
de Queiroga MA de Andrade LM Florêncio KC de Fátima Agra M da Silva MS Barbosa-Filho JM da-Cunha EV 《Fitoterapia》2004,75(3-4):423-425
The CHCl3 extract of Tillandsia recurvata yielded 5,3'-dihydroxy-6,7,8,4'-tetramethoxyflavanone (1), 1,3-di-O-cinnamoyl-glycerol (2) and ethyl ester of caffeic acid. Their structures were elucidated by means of spectroscopic methods such as mass spectroscopy and 1 and 2D-NMR. 相似文献
9.
Margaret M. Lamont Nickolas Moreno Fatima Yedith Camacho-Sánchez H. Hugo Acosta-Sánchez Scott Glaberman Miguel Angel Reyes-Lopez Ylenia Chiari 《水产资源保护:海洋与淡水生态系统》2021,31(10):3003-3010
- The Kemp’s ridley (Lepidochelys kempii) is the world’s most endangered sea turtle species. Predominately nesting at only one beach in Mexico, this species declined to an estimated 300 females in the mid-1980s. Conservation efforts in the United States and Mexico, including a head start programme in southern Texas in which hatchlings were reared in captivity for several months before being released into the wild, resulted in the recovery of this species.
- Although genetic data have previously been used to assess the success of the head start programme and dispersal of individual adults, data on immature turtles sampled at foraging areas and adult females sampled at the main nesting beach in Mexico are lacking. Genetic characterization of immature individuals is important for understanding recruitment, survival, and population demography, while genetic data on individuals from Mexico are essential for understanding dispersal and overall genetic diversity in this species.
- To address these gaps, mitochondrial DNA data were collected from 106 immature individuals sampled at four different foraging sites in the northern Gulf of Mexico and from 18 nesting females at the primary nesting beach in Mexico.
- Two previously unknown mitochondrial DNA haplotypes were discovered among the immature individuals.
- Except for these two new haplotypes, the genetic diversity of immature individuals in the northern Gulf of Mexico closely corresponds to that of adults sampled in Mexico, which suggests that much of the diversity within the nesting population can be found among immature animals dispersing to foraging grounds, including locations in the northern Gulf of Mexico.
- Continued monitoring of the genetic variation of different life stages of this species across its distribution range will help assess the success of conservation programmes by ensuring the maintenance of genetic diversity and representation of this diversity across the species’ distribution range.
10.
Carla Montanelli Alessandro Chiari Tiberio Chiari Federico Stefanini Gennarino Nascari 《Euphytica》1995,81(1):35-43
Summary Potato plantlets derived from in vitro propagation of three cultivars known for their field resistance (Cruza 148 and BR-63.65) or susceptibility (Désirée) to Pseudomonas solanacearum E.F. Smith were artificially inoculated under controlled conditions. The aim of this work was to determine the optimal inoculum concentration and the best observation period in which the cultivars would show different reactions to bacterial infection as expected on the basis of their field performance.A suitable statistical analysis of disease indices is proposed to distinguish between resistant and susceptible responses, with a particular care for the applicative needs and a univocal interpretation of the results. In order to evaluate the significance of sources of variation related to the observed mean differences, the analysis of variance and a convenient clustering procedure of disease index means were applied.The statistical analysis revealed that, under our conditions, an inoculum concentration of 5×106 cfu/plant was suitable for separating resistant from susceptible responses, in accordance with the reactions already observed in field experiments by other authors. Also, differences among the three cultivars were best observed nine to twelve days after inoculation with the pathogen. 相似文献