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Praveen C. Verma Debasis Chakrabarty Satya Narayan Jena Devesh K. Mishra Pradhyumna K. Singh Samir V. Sawant Rakesh Tuli 《Industrial Crops and Products》2009,29(2-3):581-589
Vanilla is a large genus of about 110 species in the orchid family (Orchidaceae), including the species Vanilla planifolia from which commercial vanilla flavoring is derived. Since most species of vanilla are considered rare and endangered there is an urgent need to conserve them through genetic analysis and propagation/conservation studies on this crop.The present study investigated the genetic diversity among nine leafy- and leaf-less Vanilla species employing 30 decamer RAPD primers and 10 ISSR primers. The species under study were diverse and displayed a range of variability (0–66% and 0–81% for RAPD and ISSR, respectively). A total of 154 RAPD polymorphic markers (83.24%, h = 0.378) and 93 ISSR polymorphic markers (86.11%, h = 0.363) were used to generate a genetic similarity matrix followed by the cluster analysis. Specific groupings were revealed by each cluster analysis with slight variation between two different markers. Among the nine species studied, V. planifolia, Vanilla aphylla and Vanilla tahitensis revealed very low level of variation within their collections, thus indicating a narrow genetic base. The large genetic distance of Vanilla andamanica from other species suggests its different origin. A close genetic affinity was observed between the pairs V. planifolia, V. tahitensis and Vanilla albida, V. aphylla. These are the first comparative results for RAPD and ISSR reporting inter-relationship among nine cultivated, wild and hybrid Vanilla species. 相似文献
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M. T. Shilta Narinder Kumar Chadha P. K. Pandey Paramita Banerjee Sawant 《Aquaculture International》2016,24(2):661-674
An experiment was conducted to compare the efficiency of biofilm production in natural and artificial substrates and to study their effect on water quality and growth of Etroplus suratensis. Four different substrates were used for biofilm formation: paddy straw (T1), sugarcane bagasse (T2), polyvinylchloride (PVC) pipe (T3) and plastic sheet (T4). The experiment was carried out in mud-bottomed fibre-reinforced plastic tanks (300 L) in triplicates. About 3000 cm2 surface area (600 g) of each substrate was suspended in water supplemented with fertilizers. Only cow dung and urea were applied in control tanks. The tanks were stocked with 25 fishes with average weight of 9.1 ± 0.22 g. The overall mean value of heterotrophic bacteria in substrate was found higher in straw followed by bagasse, plastic and PVC. The dominant genera of bacteria in the substrate were Bacillus, Pseudomonas and Micrococcus in that order of preponderance. The mean phytoplankton and zooplankton density on the substrates were higher in bagasse followed by straw, plastic and PVC. The biofilm developed on the substrate significantly reduced the ammonia nitrogen and nitrite-nitrogen content of water. The growth of fishes was significantly (P < 0.05) higher in substrate-based treatments than that in the control with better results in bagasse followed by straw, plastic and PVC. The conclusions of the present study are that biofilm produced on natural substrates, especially on bagasse, enhanced growth of E. suratensis and reduced the necessity of water exchange during the culture, which certainly decreases the cost of Etroplus production. 相似文献
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R T Sane S V Sawant V J Doshi J G Mhalas A K Paarikh A S Gijare D S Deshpande 《Journal of the Association of Official Analytical Chemists》1984,67(1):137-138
A simple colorimetric method is described for the determination of metaproterenol sulfate (orciprenaline sulfate). The method is based on measurement of a colored species formed when metaproterenol sulfate is treated with diazotized dapsone, p-nitroaniline, or benzocaine at room temperature, followed by treatment with an aqueous solution of trimethylamine in the case of benzocaine. Compounds such as starch, talc, and common excipients do not interfere in the reaction. Statistical validation showed that the method was precise and accurate. The results agree well with those obtained by other methods reported in the literature. 相似文献
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Anerao Jayesh Jha Vikas Shaikh Nishaat Shivalkar Apurva Nityanand Aishwarya Sawant Dishanand Rao G. Ramachandra Mangaonkar Kiran Deodhar Manjushri Desai Nitin 《Genetic Resources and Crop Evolution》2021,68(8):3161-3177
Genetic Resources and Crop Evolution - Garcinia L. is a pantropically distributed genus with high species richness in South East Asia. It is a tropical evergreen plant with distinct morphological... 相似文献
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R T Sane V J Doshi S Jukar S K Joshi S V Sawant U R Pandit 《Journal of the Association of Official Analytical Chemists》1985,68(1):83-85
A simple colorimetric method is described for the determination of thiamine hydrochloride (vitamin B1) in dosage forms. The method is based on measurement of a yellow complex formed when thiamine HCl is treated with p-methylaminophenol sulfate (Metol) under alkaline conditions. Compounds such as vitamins A, B2, B6, B12, C, D, and E, and niacinamide, citric acid, liquid glucose, calcium pantothenate, biotin, liver extract, and folic acid do not interfere in the reaction. Extracting the complex into chloroform before quantitation enhances the stability of the reaction product and removes interference of water-soluble colored constituents in syrup samples. Statistical validation shows that the method is precise and accurate. Results agree well with those obtained by other methods in the literature. 相似文献
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Quail were fed monensin to determine liver damage, as measured by changes in activities of serum enzymes and liver microsomal enzymes. Monensin fed at a therapeutic level of 110 ppm for 2 weeks produced an increase in cytochrome P-450 and cytochrome b5 and induction of the activities of benzphetamine N-demethylase, aminopyrine N-demethylase, and aniline hydroxylase, with no changes in the activities of serum sorbitol dehydrogenase (SDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). On the other hand, quail fed 110 ppm, 220 ppm, and 330 ppm monensin in feed for 6 weeks showed a significant rise in SDH and AST activities at 330 ppm but not at 110 ppm and 220 ppm. The manifestations of liver toxicity observed at 330 ppm were accompanied by a significant decrease in all the aforementioned hepatic microsomal mixed-function oxidases. In contrast, quail fed monensin at 110 ppm and 220 ppm for 6 weeks produced no change in these parameters except for benzphetamine N-demethylase, aminopyrine N-demethylase, and aniline hydroxylase, which were significantly increased in birds fed 220 ppm of monensin. 相似文献
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Aritra Bera Paramita Banerjee Sawant Subrata Dasgupta N. K. Chadha Bhawesh T. Sawant Asim Kumar Pal 《Fish physiology and biochemistry》2017,43(6):1677-1688
Diel cyclic hypoxia occurs with varying frequency and duration in freshwater habitats, yet little is known about its effects on reproduction of freshwater fishes. The present study shows that long-term exposure of goldfish (Carassius auratus) to cyclic hypoxia (0.8 ± 0.2 mg/l dissolved oxygen) for 9 h or more, per day, altered plasma lipid and sex steroid profiles, which in turn directly or indirectly suppressed ovarian growth and viable spermatozoa production. Hypoxia decreased total cholesterol and high density lipoprotein (HDL p < 0.05) and elevated triglycerides (TG; p < 0.05) in both sexes. Plasma steroid concentrations particularly of 17α-hydroxyprogesterone (17-HP), estradiol (E2), testosterone (T) in females, and T and 11-ketotestosterone (11-KT) in males were attenuated under diel hypoxic conditions. Intriguingly, both diel and continuous hypoxia elevated plasma E2 and vitellogenin levels in males. However, neither lipid nor steroid profiles recorded any variation in a dose-dependent manner in response to diel hypoxia. The reduced GSI, decreased number of tertiary oocytes, and motile spermatozoa in hypoxic fish clearly indicate suppression of gametogenesis. Thereby, prolonged diel cyclic hypoxia may affect valuable fishery resources and fish population structure by impairing reproductive performances and inducing estrogenic effects in males. 相似文献