Genetic basis of grain filling rate in wheat (Triticum aestivum L. emend. Thell.)

Summary Grain filling rate in wheat (Triticum aestivum L. emend. Thell.) positively influences grain yield under a wide range of conditions. The effective utilization of this trait in breeding depends on an understanding of its genetic control. A study was, therefore, conducted to determine the genetic basis of grain filling rate in six crosses of wheat. Higher order genic interactions and/or linkage were important in the genetic regulation of grain filling rate (GFR) in the majority of crosses. Additive ([d]) and dominance ([h]) gene effects were important in the control of GFR in main ears (ME) and whole plant ears (WPE). Additive and additive × additive epistatic effects were the most important in the genetic control of GFR in last ears (LE). Location effects on genetic effects for GFR were significant (P < 0.05) in all ear types of some crosses except in ME. Genotype × environment interaction effects were important (P < 0.001) in LE and WPE.It was concluded that the inheritance of GFR is complex and is dependent on ear type. Breeding procedures that facilitate the exploitation of non-additive and additive gene effects were recommended for the genetic improvement of grain filling rate of wheat.     

Weed community responses to soil type during transition to no-till practice on smallholder farms in Zimbabwe

Understanding drivers of weed density and diversity is essential for the development of weed management strategies. Here, we compared temporal changes in weed density and diversity under no-till (NT) and conventional (CONV) tillage systems in cotton–maize rotations on loam, clay loam and sandy loam soils immediately after transition to NT in Kadoma, Zimbabwe. The effect of tillage system on weed density varied through the growth season and was dependent upon soil type and species composition of the weed community. Although weed responses to tillage system varied amongst species, we identified general trend effects on weed density on specific soils. At 3 weeks after crop emergence (WACE), weed density on loam soils was 76% and 96% higher in NT than in CONV during the 2009/2010 and 2010/2011 seasons, respectively, and on clay loam soils it was 37% and 33% higher in NT than CONV, respectively. Weed densities in NT and CONV were similar across all soil types at 6 WACE during the 2009/2010 and 2010/2011 seasons and at 9 WACE in 2009/2010. Tillage system did not affect weed density during the growth season on sandy loam soils. Weed diversity (Shannon index) was at least 75% higher in NT than CONV on loam and clay loam soils at 3 WACE during both seasons. It is likely these increases in weed densities following conversion to NT will exacerbate already prevalent weed management problems in the smallholder sector. Earlier weeding is recommended to suppress weed emergence and reduce likely associated crop yield losses.     

Weed growth and crop yield responses to tillage and mulching under different crop rotation sequences in semi‐arid conditions

Conservation agriculture (CA) is thought to reduce weed pressure from the third year of adoption, when recommended practices are followed. Weed growth and crop yield were assessed during the third and fourth year of maize–cowpea–sorghum rotation, second and third year of maize–cowpea rotation and first and second year of maize monocropping on a clay loam soil at Matopos Research Station (annual rainfall, 573 mm) following recommended CA management practices. Each experiment had a split‐plot randomized complete block design with mouldboard plough (CONV), minimum tillage (MT) with ripper tine and planting basins as main‐plot factor and maize residue mulch rate (0, 2 and 4 t/ha) as a subplot factor, with threefold replication. All subplots were surface mulched and weeded by hoe at the same time. We hypothesized that under MT weed growth would be considerable with maize monocropping but from year 3 of CA, weed growth would decrease and crop yield increase relative to values from unmulched CONV. Minimum tillage increased weed growth in 2nd year of maize monocropping. Under the maize–cowpea rotation, the considerable weed growth in planting basins was likely due to the large intrarow spacing and poor light competiveness of the cowpea variety. Mulch contributed to weed growth being suppressed by up to 36% under CA in the maize–cowpea–sorghum rotation relative to unmulched CONV. When planted on the same date, crop yield did not differ between CA and unmulched CONV. Maize–cowpea–sorghum rotation grain yield (3143 kg/ha) was double that under monocropping , probably due to improvements in soil physical and chemical conditions.     

Early growth of pigeonpea under open-pit planting and restricted weeding in semiarid Zimbabwe

Simple and efficient tree-establishment methods are a key to the success of agroforestry technologies under limited-resource conditions. An experiment was conducted to test the effect of open pit planting and reduced weeding on soil moisture content and growth of pigeonpea (Cajanus cajan (L.) Millsp.). The results indicated that open-pit planting might reduce early growth of the plant. Clean weeding gave the most rapid growth and should be practised in the first year of establishment. However, because circle weeding substantially increased basal diameter and branching when compared to no weeding, in situations with labour constraints, it can be used to lessen the deleterious effects of weeds. This revised version was published online in June 2006 with corrections to the Cover Date.     

Molecular cloning and biochemical characterization of medaka (Oryzias latipes) lysosomal neu4 sialidase

Glycoconjugates are known to be involved in many physiological events in vertebrates. Sialidase is one of the glycosidases, which removes sialic acid from glycoconjugates. In mammals, the properties and physiological functions of sialidases have been investigated, while there is little understanding of fish sialidase. Here, to investigate the significance of fish neu4 sialidase, neu4 gene was cloned from medaka brain mRNA and identified. Sialidase-specific motifs (GPG, YRVP and Asp-Box) were well conserved in the medaka neu4 polypeptide. Optimal pH of medaka neu4 sialidase was 4.6, but its activity was sustained even at neutral and weak alkaline pH. The neu4 considerably cleaved sialic acid from 4-methylumbelliferyl-N-acetyl-α-d-neuraminic acid and sialyllactose, but not from ganglioside and fetuin, which are good substrates for human NEU4. neu4 activity was mostly detected in mitochondria/lysosome fraction after biochemical fractionation, and indirect immunofluorescence assays revealed neu4 localization in lysosome in neu4 overexpressed cells. Next, developmental change in medaka neu4 and other sialidase mRNA levels were estimated by real-time PCR. Each sialidases showed different expression patterns in embryonic development: neu4 was up-regulated at late developmental stage in embryo, and neu3a mRNA level was quite high in 0.5 dpf. On the other hand, neu3b expression was drastically increased after hatching, suggesting that each sialidase may play a different role in embryonic development.     

Advanced cycle pedigree breeding in sunflower. I: Genetic variability and testcross hybrid performance for seed yield and other agronomic traits

As a plant breeding programme matures there is a general tendency to recycle elite inbred parents, a strategy known as advanced cycle pedigree breeding. The challenge with this approach is not only to maintain genetic variability in the base breeding populations but also their usefulness for further genetic advancement in developing experimental hybrids with better performance than the commercial hybrids on the market. To assess the genetic variability and usefulness of breeding populations in sunflower (Helianthus annuus L.), 109 new inbred lines across four breeding populations Pop1, Pop2, Pop3 and Pop4 in advanced cycle pedigree breeding scheme were testcrossed to two testers to form testcross hybrid (TCH) groups: Pop1_TC, Pop2_TC, Pop3_TC and Pop4_TC. Moderate to high genetic variability along with high heritability were obtained for seed yield and oil yield within and across all TCH groups. Heritability for oil content was low to high ranging from 0.36 (Pop2_TC) to 0.81 (Pop1_TC). Genetic advance (GA%) with a 10 % selection intensity ranged from 36 % (Pop2_TC) to 42 % (Pop1_TC) for seed yield; 38 % (Pop3_TC) to 43 % (Pop1_TC) for oil yield; and 1.3 % (Pop2_TC) to 5.1 % (Pop1_TC) for oil content. To quantify the commercial potential of experimental TCHs, founder parent heterosis (FPH), mid-standard heterosis (MSH) and high standard heterosis (HSH) were calculated. Out of a total of 218 experimental TCHs evaluated, 28 had positive FPH, MPH and HSH values for oil yield representing a 13 % selection intensity as is usually applied in early generation testing.     

Fall armyworm [Spodoptera frugiperda (J.E. Smith)] damage in maize: management options for flood-recession cropping smallholder farmers

Abstract

We determined fall armyworm abundance, infestation and damage on maize in smallholder farmers’ fields in the Zambezi Valley in northern Zimbabwe. Data were collected through field measurements at four sites and interviews with 101 farmers. Fall armyworm abundance ranged from 13.7 to 33.3 larvae per 30 plants, with infestation exceeding 94% and leaf, silk and tassel damage levels ranging between 25 and 50%. Most larvae on maize plants (P?<?0.05) were at instar stages 2 to 3. Estimated grain yield decrease was 58%. Echinochloa colona and Amaranthus spp. were also damaged by fall armyworm. Farmers experimented with 28 combinations of pesticides, ash, and washing powders. Sixty five percent of the farmers used restricted to highly restricted pesticides. High fall armyworm abundance, infestation and maize damage levels suggest that the Zambezi Valley could become a fall armyworm hotspot. Sequential damage of plant parts signified the persistence of this pest in the study area. We recommend large-scale interventions that include chemical and biological control methods as well as destroying ratoon hosts, publishing information about locally available pesticides for the control of fall armyworm, and farmer training on fall armyworm biology and correct use of pesticides.     

Advanced cycle pedigree breeding in sunflower. II: combining ability for oil yield and its components

Combining ability is one of the most important information breeders use to identify superior inbred lines on the basis of their performance in hybrid combinations. The objectives of our study were (i) to quantify the importance of general combining ability (GCA) and specific combining ability (SCA) variances for seed yield, oil content and oil yield; and (ii) estimate GCA and SCA effects of seed yield, oil content and oil yield of inbred lines developed from advanced cycle pedigree breeding populations in sunflower. A total of 109 female S₃ cytoplasmic male sterile (CMS) lines from four bi-parental populations in advanced cycle pedigree breeding were crossed with two testers to form 218 testcross hybrids (TCHs). The TCHs were then evaluated in three environments. Variance component analysis results showed predominance of σ²gca over σ²sca for seed yield and oil yield indicating that superior TCHs can be identified based on positive and significant GCA effects of the female lines. For oil content σ²sca was predominant over σ²gca indicating that selecting for TCHs with high oil content would be best among line × tester combinations and not among female S₃CMS lines per se. The proportion of GCA and SCA effects in the best five TCHs in each breeding population also confirmed the predominance of GCA effects over SCA effects for seed yield and oil yield while for oil content both GCA and SCA effects appear to be important, with SCA effects having more influence than GCA. The best selection strategy would therefore be to capture the GCA in the early stages of inbreeding and then SCA for the few unique combinations when lines are almost fixed.     

Influence of duration of storage at room temperature,pre-sowing seed treatment and fruit colour harvest index on germination and seedling growth of <Emphasis Type="Italic">Jatropha curcas</Emphasis> L.

Jatropha curcas L. is being promoted by governments for greening marginal areas for rural development and biodiesel production in sub-Saharan Africa, however large gaps in knowledge on its agronomy and yield potential exist. The effect of duration of storage at room temperature, pre-sowing seed treatment of Jatropha seed and fruit colour harvest index on germination and seedling growth parameters was investigated. There was a significant negative correlation between the duration of storage of Jatropha seed and seed oil content (P < 0.001, r = ?0.99), viability (P < 0.01, r = ?0.93), percent germination (P < 0.05, r = ?0.90) and germination index (P < 0.05, r = ?0.89). The relationship between duration of seed storage and seed oil content and seed germination parameters was best described by quadratic and exponential functions which showed rapid declines in seed oil content and germination parameters in the first 3 years of storage and a slower decline between 3 and 8 years of storage. There was a significant negative correlation between duration of storage and seedling height (P < 0.01, r = ?0.97), stem diameter (P < 0.01, r = ?0.93) and dry weight (P < 0.05, r = 0.91) that was best described by quadratic functions. Seed oil content was significantly and positively correlated to seed viability (P < 0.01, r = 0.96), germination per cent (P < 0.01, r = 0.94) and germination index (P < 0.01, r = 0.93). The relationship between seed oil content and seed germination parameters was best described by exponential functions which showed an exponential increase in seed germination parameters as oil content increased above 30% and smaller increases below 30% oil content. Progressive peroxidation of lipids as seed aged explains the loss of oil from the seed. Peroxidation of lipids produced highly reactive radicals that damaged membranes and proteins reducing seed germination and seedling growth parameters as seed aged. Pre-sowing seed treatments (seed soaking and scarification) produced marginal gains in germination and seedling growth parameters in Jatropha seed stored for 3 months to 8 years. Seeds harvested at the yellow fruit maturity index, 60 days after anthesis (DAA), showed the highest seed germination percent while seed harvested at the black fruit maturity index, 75 DAA, had the highest oil content. Harvesting of Jatropha seed for oil expression is therefore recommended at the black maturity index while harvesting for seed is recommended at the yellow maturity stage. The results of this study indicate that Jatropha seed should be stored for as short a period of time as possible to maximize seed germination, seedling growth and oil yield.