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排序方式: 共有107条查询结果,搜索用时 15 毫秒
1.
Thermal inactivation of Ca2+ ATPase of squid myofibrils was significantly suppressed in the presence of Ca2+. Monomeric myosin content decreased much faster than Ca2+ ATPase inactivation in Ca medium, which was well explained by fast rod denaturation. In contrast, rod denaturation was slower
than S-1 in EDTA medium. The decrease in monomeric myosin content was explained by faster S-1 denaturation. Comparing the
S-1 and rod denaturation rates at a fixed temperature, it was concluded that S-1 denaturation was suppressed by Ca2+ whereas the rod denaturation was not. An unfolding experiment with isolated myosin rod confirmed that there was no stabilizing
effect of Ca2+ on myosin rod. It was concluded that significant stabilization of the S-1 portion by Ca2+ generated the apparently different myosin denaturation patterns in the two media. 相似文献
2.
Itaru Shioya Koji Inoue Akihisa Abe Akira Takeshita Takahiro Yamaguchi 《Fisheries Science》2011,77(5):883-889
The meat quality of farmed yellowtail Seriola quinqueradiata fed on extruded pellets (EP) containing 0.5% (v/v) red pepper (experimental group) was compared with yellowtail of the same
age fed on EP (control group). In 1-year-old yellowtail, the crude lipid content of the dorsal muscle of the experimental
group tended to be lower than that of the control group. In contrast, there was no difference in the lipid content of the
dorsal muscle between the control group and the experimental group in 2-year-old yellowtail. The muscle texture of the experimental
group was significantly firmer than that of the control group, with the effect of red pepper unrelated to fish age and lipid
content. Color change of red muscle of the experimental group was significantly lower than that of the control group, and
the content of thiobarbituric acid-reactive substances in the red muscle was significantly lower in the experimental group
than in the control group. These results are the first to demonstrate that the inclusion of red pepper in the diet is able
to reduce the loss of muscle texture firmness and to slow down color change in red muscle of yellowtail. 相似文献
3.
Decreased serum apolipoprotein A-I concentrations in cows infected with Salmonella typhimurium. 总被引:1,自引:1,他引:0
S Oikawa N Katoh H Itoh T Miyamoto M Konno T Kajita 《Canadian journal of veterinary research》1997,61(3):182-186
Serum apolipoprotein A-I concentrations in cows infected with Salmonella Typhimurium were evaluated to assess its relevance in salmonellosis. Apolipoprotein A-I has been shown in rats to be secreted by the intestine as well as the liver. Clinical symptoms such as diarrhea revealed an outbreak of salmonellosis in 22 cows on a farm, and sera were obtained at 6 (acute phase), 16, 28 (convalescent period) and 42 d (postconvalescent period) after the outbreak. Apolipoprotein A-I concentrations (mean +/- SD, mg/mL), determined by ELISA, were 0.598 +/- 0.497 (day 6), 0.111 +/- 0.060 (day 16), 0.432 +/- 0.311 (day 28) and 0.727 +/- 0.516 (day 42). Compared with the concentration at day 42, those at 16 and 28 d were significantly (P < 0.01, P < 0.05) lower, but that at day 6 was not. The serum concentration of apolipoprotein B-100 (of liver origin in cattle) was unaltered during the course of salmonellosis. The concentration of apolipoprotein A-I was positively correlated with those of serum total cholesterol (r = 0.589, P < 0.01) and phospholipids (r = 0.590, P < 0.01). These results suggest that apolipoprotein A-I in cattle is in part of intestinal origin, and also that its decreased serum concentration in salmonellosis can be attributed to the reduced intestinal synthesis or secretion of this apolipoprotein. Moreover, as a potential carrier for dietary lipids such as cholesterol, determination of serum apolipoprotein A-I concentration is suggested to be useful when assessing the nutritional status of the affected cows. 相似文献
4.
Mariko Mochizuki Masahiro Osada Katsumi Ishioka Takako Matsubara Yutaka Momota Norio Yumoto Toshinori Sako Shinji Kamiya Itaru Yoshimura 《Animal Science Journal》2014,85(3):323-329
The understanding of animal products and dairy farming is important for the promotion of dairy farming. Thus, to examine the effects of farm experience on the understanding of animal products and the management of dairy farming, the interaction between students and dairy cows was investigated in groups of first‐year veterinary nursing students in 2011 and 2012 (n = 201). These students included 181 women and 20 men. Nine items about dairy cows were presented in a questionnaire. The survey was performed before and after praxis on the educational farm attached to the authors' university. After praxis on the farm, increases occurred in the number of positive responses to the items involving the price of milk, dairy farming and the taste of milk. For these items, a significant difference (P < 0.05) was found between the scores obtained before and after training. The results of the study suggested that farm experience is useful for improving the understanding of animal products and dairy farming. 相似文献
5.
Naohiko?TakeshitaEmail author Itaru?Ikeda Norio?Onikura Masato?Nishikawa Singo?Nagata Seiichi?Matsui Seir??Kimura 《Fisheries Science》2005,71(4):784-790
ABSTRACT: The fourspine sculpin Cottus kazika is indigenous to Japan and found in Honshu except for the waters facing the Seto Inland Sea, and was also found in southern Shikoku and eastern Kyushu. This species has a catadromous lifestyle and migrates as juveniles from the sea to the middle reaches of rivers to grow. The growth pattern of this fish was investigated by a mark-and-recapture method from July 1994 to December 1996, in the Nigorikawa River, a tributary of the Gonokawa River system, Shimane Prefecture. 0-year-old fish of 50–70 mm total length ( TL ) occurred in the study area from June to July, grew to 90–140 mm TL by the following April, and attained 160–210 mm TL by December. This fish grew rapidly in September–November and April–July, almost ceasing to grow in July–September. It seems that this stagnant growth phase in summer is a characteristic of the seasonal growth pattern of C. kazika . A rearing experiment indicated that the growth rate of C. kazika was higher at 16–22°C than at 12–14 and 24–26°C. This result supports the field evidence of a stagnant growth phase in summer in the Nigorikawa River. 相似文献
6.
Makiguchi Y Konno Y Konishi K Miyoshi K Sakashita T Nii H Nakao K Ueda H 《Fish physiology and biochemistry》2011,37(2):273-284
The movements of 28 adult chum salmon, Oncorhynchus keta (Walbaum) tagged with electromyogram (EMG) transmitters were tracked along the Toyohira river, Hokkaido, Japan, in October
of 2007 and 2008 to investigate and evaluate the upstream migratory behavior through the protection bed and fishway of ground
sills. The approach time of fish that ascended successfully through the protection bed and fishway was shorter than that of
unsuccessful fish. The unsuccessful fish were observed to swim in currents with high water velocity and shallow water depth
at swimming speeds that exceeded their critical swimming speed (U
crit) during the approach to these structures. In consequence, unsuccessful fish frequently alternated between burst and maximum
sustained speeds without ever ascending the fishway, and eventually became exhausted. It is important that fishway are constructed
to enable chum salmon to find a passage way easily, so that they can migrate upstream rapidly without wasting excessive energy. 相似文献
7.
Digestion of carp myofibrils at 30 degrees C in 0.5 M KCl medium with calcium ion generated unique 135 kDa heavy meromyosin (HMM). The HMM was not produced when digested at 10 degrees C. A further digestion of the 135 kD HMM isolated in the absence of calcium ion generated uniquely short subfragment-2 (S-2) with a size of 40 kDa (40 kDa S-2) together with subfragment-1 (S-1). The 40 kDa S-2 was identified by N- and C-end sequencing, and demonstrated to locate the amino end of the rod portion. The unfolding temperature for the 40 kDa S-2 was around 52 degrees C as studied by circular dichroism measurement. The same unfolding peak was also detected with the intact rod together with a large unfolding peak at around 36 degrees C coming from the rest of the rod portion, light meromyosin. The unfolding peak for the 40 kDa S-2 in myosin was a little lower (48 degrees C) than that in free form, suggesting the involvement of the head portion in the stability of the 40 kDa S-2 in the structure. 相似文献
8.
牛体外受精卵5~10细胞期单一卵裂球染色体诊断 总被引:2,自引:0,他引:2
利用单一卵裂球的染色体诊断牛体外受精5-~10-细胞期胚胎的正常性。牛体外受精后5-~10-细胞期的胚胎用0.5%链霉蛋白酶处理分离单一卵裂球,然后用100ng/ml长春花碱处理10h,制作染色体标本。检查33枚不同发育期胚胎及卵裂球185个,有43.8%的可进行染色体分析(81/185)。结果表明,正常2倍体的发生率为46.9%,染色体异常的卵裂球中,单倍体的发生率为50.6%,显著高于多倍体(2.5%)的发生率(P<0.001)。单倍体中含有X-性染色体和含有Y-性染色体的性比基本相同。引起单倍体发生的原因可能是由于卵母细胞的孤雌发育或受精过程中精子穿入未成熟卵后由雄性原核产生。 相似文献
9.
Noguchi T Nakamura T Kimura M Zolensky ME Tanaka M Hashimoto T Konno M Nakato A Ogami T Fujimura A Abe M Yada T Mukai T Ueno M Okada T Shirai K Ishibashi Y Okazaki R 《Science (New York, N.Y.)》2011,333(6046):1121-1125
The reflectance spectra of the most abundant meteorites, ordinary chondrites, are different from those of the abundant S-type (mnemonic for siliceous) asteroids. This discrepancy has been thought to be due to space weathering, which is an alteration of the surfaces of airless bodies exposed to the space environment. Here we report evidence of space weathering on particles returned from the S-type asteroid 25143 Itokawa by the Hayabusa spacecraft. Surface modification was found in 5 out of 10 particles, which varies depending on mineral species. Sulfur-bearing Fe-rich nanoparticles exist in a thin (5 to 15 nanometers) surface layer on olivine, low-Ca pyroxene, and plagioclase, which is suggestive of vapor deposition. Sulfur-free Fe-rich nanoparticles exist deeper inside (<60 nanometers) ferromagnesian silicates. Their texture suggests formation by metamictization and in situ reduction of Fe(2+). 相似文献
10.
Chick embryos infected with Akabane virus by the yolk sac route at 6 days of incubation developed polymyositis and encephalitis. At 3 to 7 days after inoculation, skeletal muscles had myotubule degeneration, clumping of muscle cell nuclei, and infiltration of heterophils; dysplasia and aplasia were evident at 9 to 15 days after inoculation. Changes in the cerebral neostriatum and optic lobes at 2 to 11 days after inoculation included necrosis of primordial nervous tissue, hemorrhages, and hyperplasia of the vascular endothelial cells. Cavities were in nervous tissue subsequent to encephalitis. Hydranencephaly and vascular wall thickening were found 13 and 15 days after inoculation. Embryos infected intravenously at 15 days incubation had foci of encephalitis 3 to 6 days after inoculation, including neuronal degeneration, neuroglial hyperplasia, vascular endothelial proliferation, and heterophil infiltration. 相似文献