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Zakiyah Nur Meili Handoyo Tri Kim Kyung-Min 《Journal of Crop Science and Biotechnology》2019,22(1):55-63
Journal of Crop Science and Biotechnology - In this research, we investigated genetic diversity of 21 rice genotypes (Oryza sativa L.) assessed using 38 decamer RAPD primers. A total of 405 bands... 相似文献
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Fitri Irza Guari Syah Nurhasanah Handoyo Tri 《Journal of Crop Science and Biotechnology》2021,24(5):567-578
Journal of Crop Science and Biotechnology - There are many local black rice cultivars spread throughout Indonesia and are only a few of these which formally describe their genetic diversity and... 相似文献
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Proteomic analysis of wheat flour allergens 总被引:2,自引:0,他引:2
Akagawa M Handoyo T Ishii T Kumazawa S Morita N Suyama K 《Journal of agricultural and food chemistry》2007,55(17):6863-6870
Wheat can cause severe IgE-mediated systematic reactions, but knowledge on relevant wheat allergens at the molecular level is scanty. The aim of the present study was to achieve a more detailed and comprehensive characterization of the wheat allergens involved in food allergy to wheat using proteomic strategies, referred to as "allergenomics". Whole flour proteins were separated by two-dimensional gel electrophoresis with isoelectric focusing and lithium dodecyl sulfate-polyacrylamide gel electrophoresis. Then, IgE-binding proteins were detected by immunoblotting with sera of patients with a food allergy to wheat. After tryptic digestion, the peptides of IgE-binding proteins were analyzed by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry. In this study, we identified four previously reported wheat allergens or their sequentially homologous proteins [serpin, alpha-amylase inhibitor, gamma-gliadin, and low molecular weight (LMW) glutenin] by a database search. As a result of the high resolution of two-dimensional gel electrophoresis, nine subunits of LMW glutenins were identified as the most predominant IgE-binding antigens. The two-dimensional allergen map can be beneficial in many ways. It could be used, for example, for precise diagnosis of wheat-allergic patients and assessment of wheat allergens in food. Additionally, we compared allergenomics to conventional biochemical methods and evaluated the usefulness of a proteomic strategy for identifying putative allergens to wheat allergy. 相似文献
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Maulidiya Ahdatu Uli Khikamil Sugiharto Bambang Dewanti Parawita Handoyo Tri 《Journal of Crop Science and Biotechnology》2020,23(3):207-214
Journal of Crop Science and Biotechnology - The low frequency of plantlet regenerates from the somatic embryogenesis (SE) callus in sugarcane becomes a problem to produce its seed. Plant growth... 相似文献
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Wulandari Fitria Ekawati Suherman Handoyo Tri Kim Kyung-Min Sugiharto Bambang 《Journal of Crop Science and Biotechnology》2021,24(1):61-69
Journal of Crop Science and Biotechnology - The transgenic sugarcane overexpressing SoSPS1 gene increased sugar content which may alter the root exudation that interact with soil rhizosphere... 相似文献
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Sholehah Innani Mukarromatus Restanto Didik Pudji Kim Kyung-Min Handoyo Tri 《Journal of Crop Science and Biotechnology》2020,23(2):171-180
Journal of Crop Science and Biotechnology - The most important component of rice qualities are its appearance, cooking quality, eating quality, and nutritional quality. Indonesian had been... 相似文献
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Maharani Anisa Fanata Wahyu Indra Duwi Laeli Faida Nur Kim Kyung-Min Handoyo Tri 《Journal of Crop Science and Biotechnology》2020,23(1):21-28
Journal of Crop Science and Biotechnology - The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic... 相似文献
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