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Novel male-specific molecular markers (MADC5, MADC6) in hemp 总被引:8,自引:0,他引:8
Ottó Törjék Nándor Bucherna Erzsébet Kiss Hajnalka Homoki Zsuzsanna Finta-Korpelová Iván Bócsa István Nagy László E. Heszky 《Euphytica》2002,127(2):209-218
Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two
primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced.
Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa). The markers were converted into sequence-characterized amplified region (SCAR) markers. The SCAR markers correlated with
the sex of the segregating F2 population and proved the tight linkage to the male phenotype. Results of F2 plant population analysis suggest these markers are to be linked to the Y chromosome.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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