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1.
The effects of growth and leaf temperature on photosynthesis were evaluated in sweet orange seedlings ( Citrus sinensis cv. Pera) infected with Xylella fastidiosa (the bacterium that causes citrus variegated chlorosis, CVC). Measurements of leaf gas exchange and chlorophyll a fluorescence were taken at leaf temperatures of 25, 30, 35 and 40°C in healthy and infected (without visible symptoms) seedlings submitted to two temperature regimes (25/20 or 35/20°C, day/night), not simultaneously. The CO2 assimilation rates ( A ) and stomatal conductance ( g s ) were higher in healthy plants in both temperature regimes. Values for A and g s of infected and healthy plants were higher in the 35/20°C regime, decreasing with leaf temperature increase. In addition, differences between healthy and infected plants were higher at 35/20°C, while no differences in chlorophyll a fluorescence parameters were observed except for potential quantum efficiency of photosystem II, which was higher in infected plants. Low A values in infected plants were caused by low g s and probably by biochemical damage to photosynthesis. The high alternative electron sink of infected plants was another effect of reduced A . Both high growth and high leaf temperatures increased differences in A between healthy and infected plants. Therefore this feature may be partially responsible for lower growth and/or productivity of CVC-affected plants in regions with high air temperature. 相似文献
2.
3.
Renato Beozzo Bassanezi Luiz Henrique Montesino Eduardo Sanches Stuchi 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(4):565-572
Citrus huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus and Ca. L. americanus and transmitted by the Asian psyllid Diaphorina citri, is the most serious disease of citrus worldwide because of crop devastation and difficulty to control. Since 2004, approximately 3 million trees were eliminated in attempts to limit its spread in Brazil. Where HLB becomes endemic, the disease progression in the orchard and the increasing symptom severity throughout the tree canopy can be relatively fast, greatly reducing the economic life of affected orchards because of tree decline and yield reduction. Although the majority of the fruit from symptomatic branches drop before harvest, a significant amount of affected fruit remain attached, are available for harvest, and can affect juice quality. To quantify and compare the effects of HLB on fruit quality of the most important sweet orange cultivars grown in São Paulo State, 4–6 year-old sweet orange trees from 26 blocks (two of ‘Valencia Americana’, eight of ‘Hamlin’, four of ‘Westin’, seven of ‘Pera’, and five of ‘Valencia’) were selected prior to harvest. In each block, 14–21 HLB-symptomatic trees were chosen. In each tree, the quality of 20 fruit normal in appearance from asymptomatic branches and 20 symptomatic fruit from symptomatic branches were assessed. In general, compared to normal fruit, the symptomatic fruit were small, light, more acidic, and had lower juice percentage, Brix, total soluble solids per box, total soluble solids per fruit, and Brix/acidity ratio. These effects of fruit quality were less pronounced on early and mid season sweet orange cultivars than on late season cv. Valencia. 相似文献
4.
Alkaloids are a large and structurally diverse group of marine-derived natural products. Most marine-derived alkaloids are biologically active and show promising applications in modern (agro)chemical, pharmaceutical, and fine chemical industries. Different approaches have been established to access these marine-derived alkaloids. Among these employed methods, biotechnological approaches, namely, (chemo)enzymatic synthesis, have significant potential for playing a central role in alkaloid production on an industrial scale. In this review, we discuss research progress on marine-derived alkaloid synthesis via enzymatic reactions and note the advantages and disadvantages of their applications for industrial production, as well as green chemistry for marine natural product research. 相似文献
5.
Oceans are a rich source of structurally unique bioactive compounds from the perspective of potential therapeutic agents. Marine peptides are a particularly interesting group of secondary metabolites because of their chemistry and wide range of biological activities. Among them, cyclic peptides exhibit a broad spectrum of antimicrobial activities, including against bacteria, protozoa, fungi, and viruses. Moreover, there are several examples of marine cyclic peptides revealing interesting antimicrobial activities against numerous drug-resistant bacteria and fungi, making these compounds a very promising resource in the search for novel antimicrobial agents to revert multidrug-resistance. This review summarizes 174 marine cyclic peptides with antibacterial, antifungal, antiparasitic, or antiviral properties. These natural products were categorized according to their sources—sponges, mollusks, crustaceans, crabs, marine bacteria, and fungi—and chemical structure—cyclic peptides and depsipeptides. The antimicrobial activities, including against drug-resistant microorganisms, unusual structural characteristics, and hits more advanced in (pre)clinical studies, are highlighted. Nocathiacins I–III (91–93), unnarmicins A (114) and C (115), sclerotides A (160) and B (161), and plitidepsin (174) can be highlighted considering not only their high antimicrobial potency in vitro, but also for their promising in vivo results. Marine cyclic peptides are also interesting models for molecular modifications and/or total synthesis to obtain more potent compounds, with improved properties and in higher quantity. Solid-phase Fmoc- and Boc-protection chemistry is the major synthetic strategy to obtain marine cyclic peptides with antimicrobial properties, and key examples are presented guiding microbiologist and medicinal chemists to the discovery of new antimicrobial drug candidates from marine sources. 相似文献
6.
Fernando González-Andrés Pedro A. Casquero Cristina San-Pedro Elías Hernández-Sánchez 《Genetic Resources and Crop Evolution》2007,54(1):27-44
All the accessions (35) of white lupin (Lupinus albus L.) landraces collected from northwest Iberian plateau and maintained at the Spanish germplasm collection (CRF of INIA),
were studied with the general objective of assessing the biodiversity of these landraces and to ascertain their value as genetic
resources for the development of germplasm adapted to the areas where they were collected with long and chilly winters. The
characterization study comprised 51 qualitative and 50 quantitative characters. Quantitative parameters were analyzed by Principal
Components Analysis (PCA). The 2-dimensional plot (49.3% of cumulative variability) formed a main group of accessions and
4 outliers (#1, #9, #27 and #28) separated in the first PC. The characters with a higher contribution to the first PC were
inflorescence length, leaf petiole length and leaf central foliole area. The presence of alkaloids, the percentage of plants
killed by soil borne fungal diseases, the phenology, the yield per plant and yield components were also assessed. Twenty six
accessions (the main group in the PCA plot) showed high homogeneity, with the following highlights: bitterness, indeterminate
growth habit, early ripeness and spring sowing with the exception of #18, in which seeding date is unknown and it had a later
phenology. By contrast accessions #9 and #27 were bitter of autumn sowing with determinate growing habit and they had the
longest values of primary inflorescence length and a later phenology when they were sown in spring. Average values for yield
and yield components showed a broad range of variation between accessions. The main yield component was the number of pods
per plant. Accession #22 had the highest mean seed mass, although this had not significant influence in yield. Accession #17
showed the highest yield and #1 the lowest. This last also had the lowest values of inflorescence length, leaf petiole length
and leaf central foliole area. No significant differences of resistance to soil-borne fungi were found between accessions.
The year had a significant effect in the phenology, yield and yield components except for number of seeds per pod. The studied
material might be of interest for the development of spring sowing germplasm adapted to north Iberian peninsula. 相似文献
7.
Since the late 1980s, a large number of depsipeptides that contain a new topography, referred to as “head-to-side-chain” cyclodepsipeptides, have been isolated and characterized. These peptides present a unique structural arrangement that comprises a macrocyclic region closed through an ester bond between the C-terminus and a β-hydroxyl group, and terminated with a polyketide moiety or a more simple branched aliphatic acid. This structural pattern, the presence of unique and complex residues, and relevant bioactivity are the main features shared by all the members of this new class of depsipeptides, which are reviewed herein. 相似文献
8.
Fernando Snchez‐Dvila Rodolfo Ungerfeld Alejandro Sergio del Bosque‐Gonzlez Hugo Bernal‐Barragn 《Reproduction in domestic animals》2019,54(2):391-400
The aim of this study was to determine the influence of the season of birth on the body weight, testicular size, testosterone concentrations, semen output and sexual behaviour in Saint Croix male lambs. Thirty‐three lambs were born in spring (n = 10), summer (Sum; n = 12) or autumn (n = 11). Body weight and the scrotal circumference were weekly recorded; semen was also weekly collected by electroejaculation; and sexual behaviour towards oestrous ewes was weekly evaluated. Data were recorded from 15 to 33 weeks of age. The reproductive development of Saint Croix male lambs differed according to the period in which they were born. More lambs born in spring than in summer and autumn achieved puberty during this study and were also more precocious in semen production and displayed sexual behaviour earlier. Lambs born in summer delayed their semen production; however, season‐related negative effects were less pronounced at the end of the study than in autumn born lambs born. In conclusion, despite the apparently low seasonality of the breed, Saint Croix male lambs showed in this study a seasonal pattern in their reproductive development at least until they achieve the puberty. 相似文献
9.
Conceição FR Dellagostin OA Paolichi F Leturia AC Gil Turnes C 《Veterinary journal (London, England : 1997)》2004,167(1):53-58
The molecular profile of 30 Moraxella bovis strains, recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001, was determined through randomly applied polymorphic DNA (RAPD) analysis. Molecular profiles of nine strains recovered after 1990 varied from those recovered before 1990. The profiles of 13 strains (48%) differed from those of three vaccinal strains extensively used since 1984 in Argentina and Uruguay. Eight Argentinean strains, one from Brazil and two from Uruguay had identical RAPD profiles. Strains belonging to different serogroups had identical RAPD profiles, demonstrating that this technique was not able to discriminate among strains with low cross-reactivity indices. RAPD may be helpful in the primary characterization of M. bovis strains, but it does not replace serological characterization. 相似文献
10.
María Fuentes-Rubio José J. Cerón Carlos de Torre Damián Escribano Ana M. Gutiérrez Fernando Tecles 《Canadian journal of veterinary research》2014,78(2):127-132
The purpose of this research was to study changes in the salivary proteome of healthy pigs in stressful situations to identify any potential new salivary biomarker of stress. Three groups of animals were subjected to 3 stress models: snaring restraint followed by simulated sampling of vena cava blood; brief transport by road; and restriction of movement in a digestibility cage. Saliva was obtained from each animal before and 15 and 30 min after the induction of stress. The samples from the animals that showed the greatest increase in salivary cortisol concentration were pooled and run on 2-dimensional gels. Coomassie Brilliant Blue R-250 was used for spot detection and mass spectrometry for spot identification. Statistical analyses showed that 2 proteins had significant differences in expression before and after the induction of stress. These proteins were identified as odorant-binding protein and fragments of albumin. Further studies will be necessary to confirm the value of using these proteins as salivary biomarkers of stress in pigs. 相似文献