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1.
Chang‐Hoon Shin Hien Thi Dieu Bui Samad Rahimnejad Ji‐Hoon Cha Byung‐Woo Yoo Bo‐Kyeun Lee Hyung‐Jin Ahn Soo‐Il Choi Yun‐Jeong Choi Yong‐Ho Park Jeong‐Dae Kim Kyeong‐Jun Lee 《Journal of the World Aquaculture Society》2014,45(3):258-268
This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets. 相似文献
2.
Pantu Kumar Roy Ahmad Yar Qamar Xun Fang Ghangyong Kim Seonggyu Bang Mahanama De Zoysa Sang Tae Shin Jongki Cho 《Reproduction in domestic animals》2021,56(2):342-350
Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress. 相似文献
3.
Hyunseok Kim Min Jang Chi Won Shin Hyungjoo Han Jeesoo Cha Inhyung Lee Won-gyun Son 《Veterinary anaesthesia and analgesia》2021,48(1):92-100
ObjectiveTo describe an approach for ethmoidal nerve block (EBLOCK) and to compare the effects of a maxillary nerve block (MBLOCK), EBLOCK and their combination (M-EBLOCK) on heart rate (HR), systolic (SAP), mean (MAP), diastolic (DAP) arterial pressures and respiratory rate (fR) during nasal stimulation in dogs.Study designProspective, blinded, randomized, crossover placebo-controlled study.AnimalsBeagle dogs (five cadavers, nine live dogs), with a median (interquartile range) weight of 10.5 (10.3–11.0) kg.MethodsThe accuracy of iohexol injections (each 1 mL) at the maxillary and ethmoidal foramina in cadavers was evaluated using computed tomography. Then, anesthetized dogs were administered four bilateral treatments separated by 1 week, saline or 2% lidocaine 1 mL per injection: injections of saline at the maxillary and ethmoidal foramina (Control), injections of lidocaine at the maxillary foramina and saline at the ethmoidal foramina (MBLOCK), injections of saline at the maxillary foramina and lidocaine at the ethmoidal foramina (EBLOCK) and injections of lidocaine at all foramina (M-EBLOCK). The ventral nasal meatus was bilaterally stimulated using cotton swabs, and HR, SAP, MAP, DAP and fR were continuously recorded. Values for each variable were compared before and after stimulation using Wilcoxon signed-rank test. Changes in variables among treatments were analyzed using Mann–Whitney U and Kruskal–Wallis tests (p ≤ 0.05).ResultsComputed tomography revealed iohexol distribution around the openings of the target foramina in all cadavers. In living dogs, HR, SAP, MAP, DAP and fR significantly increased after stimulation within each treatment (p < 0.03). Physiologic responses were significantly attenuated, but not absent, in the M-EBLOCK [HR (p = 0.019), SAP, MAP, DAP and fR (all p ≤ 0.001)] compared with those in the Control.Conclusions and clinical relevanceConcurrent injections of lidocaine at the maxillary and ethmoidal foramina attenuated HR, arterial pressure and fR responses to nasal stimulation in Beagle dogs. 相似文献
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利用太阳能空气集热器、干燥仓、传感系统、控制系统、鼓风机等自行设计了太阳能干燥平台,在此平台上进行热研2号柱花草低温干燥试验,研究实时太阳辐射下的集热器接收辐射强度、干燥仓进出口温度、相对湿度、温度差、湿度差等对柱花草干燥的影响。试验结果表明:牧草干燥大致分为3个阶段,牧草含水率在3个阶段的干燥速率约为0.243、0.140 5、0.039 2%/min,牧草干燥效率较自然日晒显著提高。通过干燥参数和牧草质量变化的散点图,建立多元线性线性回归方程,相关系数为0.986 5,与试验结果符合良好,有助于进一步对干燥工艺进行优化。 相似文献
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辣椒倍半萜植保素代谢的分子生态学研究 总被引:1,自引:0,他引:1
为建立克隆辣椒倍半萜环化酶基因的cDNA文库,我们尝试了用紫外线(UV)处理离体辣椒叶片,考察UV对辣椒倍半萜环化酶活性的影响,结果表明UV能诱导辣椒叶片表达倍半萜环化酶活性;不同抗性水平的辣椒品种倍半萜环化酶活性对紫外线的反应有差异,紫外线处理后,抗病品种cm334在24
h 出现酶活性高峰,而感病品种subiche在36~42 h出现酶活性高峰;
在紫外线处理后0~24 h cm334的酶活性高于subiche。UV对辣椒倍半萜环化酶活性的诱导作用与UV的作用方式、剂量及辣椒植株的生育期有关,
紫外线交联处理45 s诱导10叶期的倍半萜环化酶活性最高;5支15 W紫外灯相距20
cm照射15 min低于1支相同的紫外灯处理;在相同的UV作用下,开花前辣椒叶片倍半萜环化酶活性显著高于开花以后的辣椒叶片。另一方面,紫外线处理是辣椒叶片中的鲨烯合成酶活性受到一定程度的拟制。 相似文献
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9.
Kihoon Kim Jong Yoon Lee Hyun Kim Jaiho Shin Yongwon Shin Young Tai Yoo Hwi-Yool Kim 《Fibers and Polymers》2017,18(2):231-239
In recent decades, tremendous research has focused on the production of nanoscale fibers using synthetic polymers, with the goal of fabricating nanofibrous scaffolds for wound healing. However, the hydrophobicity of such polymers typically hinders attachment and proliferation of the cells. In this study, we combined poly-d,l-lactide-co-glycolide (PLGA) and small intestine submucosa (SIS) to fabricate blended nanofibers for wound healing by electrospinning. PLGA and SIS were dissolved in 1,1,1,3,3,3-hexafluoro isopropanol to produce different weight ratios of PLGA/SIS-blended nanofibrous membranes (NFM). Physicochemical characterization of the electrospun NFM was performed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, water contact angle analysis, degradation test and tensile testing. The PLGA/SIS-blended NFM showed improved hydrophilicity and tensile strength. Better infiltration, attachment and proliferation of rat granulation fibroblasts of PLGA/SIS-blended NFMs compared to PLGA NFMs were identified by morphological differences determined by SEM and a water-soluble tetrazolium salt assay kit. Based on our results, the PLGA/SIS blended NFMs were found to be suitable for use as a potential material for wound dressing. 相似文献
10.
Yang DK Shin EK Oh YI Kang HK Lee KW Cho SD Song JY 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1077-1082
We analyzed the nucleotide sequences of the G-L (glycoprotein-large protein) intergenic non-coding region of 33 strains of the rabies virus (RABV) isolated in South Korea in 1998-2010 and compared the sequences with those of previously reported non-Korean strains. The similarities of the nucleotide sequences of the G-L region among all Korean RABV isolates ranged from 97.1 to 100%. Based on the phylogenetic analysis of the G-L region, the Korean RABV isolates were classified into three distinct subgroups with high similarity and were most closely related to the non-Korean NeiMeng1025C isolate, which was isolated from a rabid raccoon dog in eastern China, suggesting that the Korean RABV isolates originate from a rabid raccoon dog in northeastern Asia. Our results indicated that G-L region, as a useful phylogenetic indicator, is equivalent to the nucleoprotein (N) or glycoprotein (G) gene for study of RABV molecular epidemiology and that the Korean RABV isolates showing a few substitutions in the G-L region are continuously circulating in South Korea. 相似文献