Selection for Seed Yield in Lolium perenne L.*

Components of seed yield were estimated for each set of 100 plants derived from six varieties of Lolium perenne. Significant differences between the varieties were found for all components of seed yield, but the range was broader within each variety. An indirect selection of seed yield using the character seeds per spike produced a selection response similar to that obtained by direct selection on high seed yield per plant. A direct and indirect selection of characters estimated in small, drilled plots of topcross off-springs (F₁) was less successful than the selection based on the single plants. Spearman rank correlation coefficients between the single plants and their offsprings did not show significant agreement for seed yield. But altogether the results indicated that the increase of seed yield/area was about 6 % when 10 % of the best plants were selected.     

A circadian rhythm orchestrated by histone deacetylase 3 controls hepatic lipid metabolism

Disruption of the circadian clock exacerbates metabolic diseases, including obesity and diabetes. We show that histone deacetylase 3 (HDAC3) recruitment to the genome displays a circadian rhythm in mouse liver. Histone acetylation is inversely related to HDAC3 binding, and this rhythm is lost when HDAC3 is absent. Although amounts of HDAC3 are constant, its genomic recruitment in liver corresponds to the expression pattern of the circadian nuclear receptor Rev-erbα. Rev-erbα colocalizes with HDAC3 near genes regulating lipid metabolism, and deletion of HDAC3 or Rev-erbα in mouse liver causes hepatic steatosis. Thus, genomic recruitment of HDAC3 by Rev-erbα directs a circadian rhythm of histone acetylation and gene expression required for normal hepatic lipid homeostasis.     

The effect of autumn ridging and inter-row subsoiling on potato tuber yield and quality on a sandy soil in Denmark

Autumn ridging is a modified version of the ridge tillage system. Instead of setting up ridges during the growing season, they are established in autumn and left for the winter. Previous studies have documented positive effects of autumn ridging on potato yield and we hypothesized that subsoiling could enhance these effects. To determine the effect of autumn ridging and inter-row subsoiling on potato yield and quality a field experiment was conducted on sandy soil from 2001 to 2003. Autumn ridging resulted in an average total and marketable tuber yield of 25.6 and 9.2 t ha⁻¹, which was not significantly different from the average total and marketable yield of 25.6 and 8.9 t ha⁻¹ with ploughing. However, autumn ridging significantly reduced the incidence of black scurf from 2.5% to 2.2%. Inter-row subsoiling in the growing season significantly increased marketable potato tuber yield from 8.4 to 9.6 t ha⁻¹ and reduced the occurrence of malformed potatoes from 9.3% to 7.5%, irrespective of tillage treatment and irrigation level. There was no significant interaction between autumn ridging and subsoiling. The beneficial effect of subsoiling on marketable yield was driven by a 48.5% increase in the dry year of 2001. Subsoiling reduced the incidence of common scab from 7.8% to 6.9% when irrigation was reduced. It is concluded that at least three factors may modify the effects of subsoiling: Soil water status in the growing season, precipitation immediately before and after the subsoiling operation, and crop growth stage at the time of subsoiling.     

Stickstoffausnutzungsvermögen von Lolium perenne und Lolium multiflorum-Sorten

Selection for improved nitrogen-utilization in Lolium
In a 3-year study the responses of 14 Lolium perenne-varieties and 14 of Lolium multiflorum to different nitrogen fertilization (75, 150, 300 kg N/ha) were investigated. The varieties were classificated into four types of nitrogen utilization. "Low input varieties" can be found in type III. The varieties of this type produced always the highest yield at all levels of nitrogen fertilization. The yield of type I was always smaller than the average of all varieties. The varieties of type II gave significant better responses than the trial average up to 150 kg N/ha. Under low and high N-fertilization the type III can be separate of type I and II because it is always the outstanding variety. The dry matter production of the roots did not show varieties differences under the increasing N-fertilization. The soil nitrate contents were with 20 kg nitrate nitrogen per ha very small.     

Ridging in autumn as an alternative to mouldboard ploughing in a humid-temperate region

In the original ridge tillage system as practiced in the US Corn Belt, ridges are formed during the growing season. Several studies have documented that this can reduce leaching of nutrients and improve fertilizer efficiency. This study was conducted to determine whether ridges formed in autumn can be used as an alternative to ploughing to reduce N leaching during autumn and winter, and thereby increase growth and N uptake of a subsequent unfertilized crop. A factorial field experiment with tillage and residues as factors was conducted during 1998–2000. Tillage treatments were autumn ridging and ploughing. Residue treatments were stubble, stubble + straw and stubble + liquid manure in order to create a gradient of C/N ratios. From the time of harvest until planting of a subsequent barley crop (Hordeum vulgare L.), inorganic N was determined 11 times in 1998–1999 and 10 times in 1999–2000 in the 0–10, 10–30, 30–60 and 60–90 cm soil layers. Growth and N uptake of barley was quantified nine times in both 1999 and 2000. Barley grain and straw yields were determined. Ridging resulted in lower levels of inorganic N in the 30–90 cm soil layer in November and a significantly higher level of inorganic N in the 0–30 cm soil layer in late April, indicating reduced leaching and increased N availability for the subsequent crop. Ridging significantly increased growth, yield and N uptake of barley whereas incorporation of straw generally reduced growth, yield and N uptake. It is concluded that ridging in autumn has a N conserving effect, and it is suggested that the potential of ridging in autumn as an alternative to ploughing is further investigated in detailed studies of solute movement, N immobilization/mineralization and crop performance.     

Harvesting European knowledge on soil functions and land management using multi‐criteria decision analysis

Soil and its ecosystem functions play a societal role in securing sustainable food production while safeguarding natural resources. A functional land management framework has been proposed to optimize the agro‐environmental outputs from the land and specifically the supply and demand of soil functions such as (a) primary productivity, (b) carbon sequestration, (c) water purification and regulation, (d) biodiversity and (e) nutrient cycling, for which soil knowledge is essential. From the outset, the LANDMARK multi‐actor research project integrates harvested knowledge from local, national and European stakeholders to develop such guidelines, creating a sense of ownership, trust and reciprocity of the outcomes. About 470 stakeholders from five European countries participated in 32 structured workshops covering multiple land uses in six climatic zones. The harmonized results include stakeholders’ priorities and concerns, perceptions on soil quality and functions, implementation of tools, management techniques, indicators and monitoring, activities and policies, knowledge gaps and ideas. Multi‐criteria decision analysis was used for data analysis. Two qualitative models were developed using Decision EXpert methodology to evaluate “knowledge” and “needs”. Soil quality perceptions differed across workshops, depending on the stakeholder level and regionally established terminologies. Stakeholders had good inherent knowledge about soil functioning, but several gaps were identified. In terms of critical requirements, stakeholders defined high technical, activity and policy needs in (a) financial incentives, (b) credible information on improving more sustainable management practices, (c) locally relevant advice, (d) farmers’ discussion groups, (e) training programmes, (f) funding for applied research and monitoring, and (g) strengthening soil science in education.     

Clinical trial of three therapeutic regimens for bovine mastitis

An open, block randomised multi-centre clinical trial was performed in Norway during 1985 to 1987 to compare the therapeutic efficacy of three antibiotic regimens against clinical bovine mastitis caused by penicillin-sensitive bacteria. Two regimens consisted of procaine penicillin injected intramuscularly for either three or five days, and the third, the traditional Norwegian regimen, consisted of one intramuscular injection of a combination of procaine penicillin and dihydrostreptomycin followed by one intramammary treatment daily per infected quarter for four days. The study included 621 quarters with infectious mastitis from 439 cows. The most efficient regimen for all bacteria was five days systemic treatment (53.1 per cent cured), and the traditional regimen was second best (46.7 per cent cured). The least efficient regimen consisted of systemic therapy with procaine penicillin for three days (36.9 per cent cured). The difference between the therapeutic efficacies of the three regimens was reduced when the clinical mastitis was severe, and in severe mastitis caused by Staphylococcus aureus the difference was very small.     

Kemink subsoiling before and after planting

The Kemink exact soil management system is a non-inversion soil management system based on subsoiling, ridges and controlled traffic. Previous studies have documented benefits of the Kemink system used in its entirety, but the isolated effect of Kemink subsoiling has not been investigated before. To determine the isolated effect of Kemink subsoiling before and after planting two field experiments in sugar beet and barley were conducted in 1999 and 2000 under low nutrient input conditions in a conventional soil management system without recognized compaction problems. Kemink subsoiling after planting generally showed a negative effect on the growth and yield of both crops, whereas subsoiling before planting increased sugar beet yield from 8.4 to 9.5 t ha⁻¹ and sugar beet nitrogen uptake from 48.5 to 57.4 kg ha⁻¹. There was no effect of subsoiling before planting on the grain yield of barley. The negative effect of subsoiling after planting was more pronounced in 1999 than in 2000, and more pronounced in spring barley. The study shows that Kemink subsoiling after planting involves a significant risk of crop damage and cannot be expected to improve crop performance in conventional soil management systems in its current form, whereas Kemink subsoiling before planting may have potential as a measure to increase yield of sugar beet and possibly other row crops too, under low nutrient input conditions.     

Ermittlung geeigneter Selektionskriterien zur Verbesserung der Persistenz beim Deutschen Weidelgras

Screening for persistence in Lolium perenne L.
The assessment of persistence, as well as yield potential, is fundamentally important for cultivar evaluation of perennial grasses. But it is difficult to estimate the persistence because of limited time available for testing new varieties. The aim of this study was to find out conditions for effective and quick selection for persistence in Lolium perenne. Four strains of Lolium perenne were investigated for three years in the following split pot design with 3 replications: 180–220 kg N/ha or 360–440 kg N/ha and year, 3 or 6 cuts/year and 3–4 cm or 7–8 cm cutting height.
Yield, regrowth (score 0–9, 7 days after each cut) and persistence (number of non-surviving ramets) were determined for each ramet of all clones and for each plot of the strains. The dry matter yield was reduced to about 50 % between the first and the third year of the investigation. The differences in the dry matter yield between the four strains have been more evident in the six than in the three cutting treatment.
Already at the end of the first year of the investigation the strains differed significantly in the regeneration in the six cut treatment. The regrowth of strain III showed a significant reduction compared with the other three strains.
A significant reduction of the surviving ramets was first observed at the end of the second year of the investigation in the six cut treatment only, but between the strains were no significant differences.
These results demonstrate, that an investigation on single plants over a period of three years cannot give an exactly prediction about the persistence of Lolium perenne strains.